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Acetylcholinesterase modulates neurite outgrowth on fibronectin.
Biochem Biophys Res Commun. 2007 May 04; 356(2):398-404.BB

Abstract

Acetylcholinesterase (AChE) has been reported to be involved in the modulation of neurite outgrowth. To understand the role played by different domains, we transfected neuroblastoma cells with three constructs containing the invariant region of AChE, differing in the exon encoding the C-terminus and therefore in AChE cellular fate and localization. All isoforms increased neurite extension, suggesting the involvement of the invariant domain [A. De Jaco, G. Augusti-Tocco, S. Biagioni, Alternative AChE molecular forms exhibit similar ability to induce neurite outgrowth, J. Neurosci. Res. 70 (2002) 756-765]. The peripheral anionic site (PAS) is encoded by invariant exons and represents the domain involved in non-cholinergic functions of AChE. Masking of PAS with fasciculin results in a significant decrease of neurite outgrowth in all clones overexpressing AChE. A strong reduction was also observed when clones were cultured on fibronectin. Treatment of clones with fasciculin, therefore masking PAS, abolished the fibronectin-induced reduction. The inhibition of the catalytic site cannot revert the fibronectin effect. Finally, when clones were cultured on fibronectin in the presence of heparin, a ligand of fibronectin, the inhibitory effect was completely reversed. Our results indicate that PAS could directly or indirectly mediate AChE/fibronectin interactions.

Authors+Show Affiliations

Dipartimento Biologia Cellulare e Sviluppo, Università La Sapienza, Piazzale A. Moro 5, 00185 Roma, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17359933

Citation

Giordano, C, et al. "Acetylcholinesterase Modulates Neurite Outgrowth On Fibronectin." Biochemical and Biophysical Research Communications, vol. 356, no. 2, 2007, pp. 398-404.
Giordano C, Poiana G, Augusti-Tocco G, et al. Acetylcholinesterase modulates neurite outgrowth on fibronectin. Biochem Biophys Res Commun. 2007;356(2):398-404.
Giordano, C., Poiana, G., Augusti-Tocco, G., & Biagioni, S. (2007). Acetylcholinesterase modulates neurite outgrowth on fibronectin. Biochemical and Biophysical Research Communications, 356(2), 398-404.
Giordano C, et al. Acetylcholinesterase Modulates Neurite Outgrowth On Fibronectin. Biochem Biophys Res Commun. 2007 May 4;356(2):398-404. PubMed PMID: 17359933.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acetylcholinesterase modulates neurite outgrowth on fibronectin. AU - Giordano,C, AU - Poiana,G, AU - Augusti-Tocco,G, AU - Biagioni,S, Y1 - 2007/03/06/ PY - 2007/02/16/received PY - 2007/02/27/accepted PY - 2007/3/16/pubmed PY - 2007/6/6/medline PY - 2007/3/16/entrez SP - 398 EP - 404 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 356 IS - 2 N2 - Acetylcholinesterase (AChE) has been reported to be involved in the modulation of neurite outgrowth. To understand the role played by different domains, we transfected neuroblastoma cells with three constructs containing the invariant region of AChE, differing in the exon encoding the C-terminus and therefore in AChE cellular fate and localization. All isoforms increased neurite extension, suggesting the involvement of the invariant domain [A. De Jaco, G. Augusti-Tocco, S. Biagioni, Alternative AChE molecular forms exhibit similar ability to induce neurite outgrowth, J. Neurosci. Res. 70 (2002) 756-765]. The peripheral anionic site (PAS) is encoded by invariant exons and represents the domain involved in non-cholinergic functions of AChE. Masking of PAS with fasciculin results in a significant decrease of neurite outgrowth in all clones overexpressing AChE. A strong reduction was also observed when clones were cultured on fibronectin. Treatment of clones with fasciculin, therefore masking PAS, abolished the fibronectin-induced reduction. The inhibition of the catalytic site cannot revert the fibronectin effect. Finally, when clones were cultured on fibronectin in the presence of heparin, a ligand of fibronectin, the inhibitory effect was completely reversed. Our results indicate that PAS could directly or indirectly mediate AChE/fibronectin interactions. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/17359933/Acetylcholinesterase_modulates_neurite_outgrowth_on_fibronectin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(07)00436-6 DB - PRIME DP - Unbound Medicine ER -