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Studies on genome relationship and species-specific PCR marker for Dasypyrum breviaristatum in Triticeae.
Hereditas. 2006 Dec; 143(2006):47-54.H

Abstract

Dasypyrum breviaristatum and nine related species in Triticeae were analyzed using the random amplified polymorphic DNA (RAPD) technique, in order to understand the genetic relationship and to develop species specific markers. The genome relationship dendrogram shows that D. breviaristatum and D. villosum could not be grouped together, indicating that D. breviaristatum was unlikely to be directly derived from D. villosum, while D. breviaristatum was closest to Thinopyrum intermedium, which implied that they might have similar breeding behaviors when introducing their chromatins into wheat. A D. breviaristatum genome specific RAPD product of 1182bp, was cloned and designated as pDb12H. Sequence analysis revealed that pDb12H was strongly homologuos to a long terminal repeat (LTR) Sabrina retrotransposon newly reported in Hordeum. The pDb12H was converted into a PCR based marker, which allows effectively monitoring the D. breviaristatum chromatin introgression into wheat. Fluorescence in situ hybridization (FISH) suggested that pDb12H was specifically hybridized throughout all D. breviaristatum chromosomes arms except for the terminal and centromeric regions, which can be used to characterize wheat -D. breviaristatum chromosome translocation. The genomes repetitive element will also be useful to study gene interactions between the wheat and alien genomes after the polyploidization.

Authors+Show Affiliations

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, PR China. yangzujun@uestc.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17362333

Citation

Yang, Zu-Jun, et al. "Studies On Genome Relationship and Species-specific PCR Marker for Dasypyrum Breviaristatum in Triticeae." Hereditas, vol. 143, no. 2006, 2006, pp. 47-54.
Yang ZJ, Liu C, Feng J, et al. Studies on genome relationship and species-specific PCR marker for Dasypyrum breviaristatum in Triticeae. Hereditas. 2006;143(2006):47-54.
Yang, Z. J., Liu, C., Feng, J., Li, G. R., Zhou, J. P., Deng, K. J., & Ren, Z. L. (2006). Studies on genome relationship and species-specific PCR marker for Dasypyrum breviaristatum in Triticeae. Hereditas, 143(2006), 47-54.
Yang ZJ, et al. Studies On Genome Relationship and Species-specific PCR Marker for Dasypyrum Breviaristatum in Triticeae. Hereditas. 2006;143(2006):47-54. PubMed PMID: 17362333.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Studies on genome relationship and species-specific PCR marker for Dasypyrum breviaristatum in Triticeae. AU - Yang,Zu-Jun, AU - Liu,Cheng, AU - Feng,Juan, AU - Li,Guang-Rong, AU - Zhou,Jian-Ping, AU - Deng,Ke-Jun, AU - Ren,Zheng-Long, PY - 2007/3/17/pubmed PY - 2007/5/15/medline PY - 2007/3/17/entrez SP - 47 EP - 54 JF - Hereditas JO - Hereditas VL - 143 IS - 2006 N2 - Dasypyrum breviaristatum and nine related species in Triticeae were analyzed using the random amplified polymorphic DNA (RAPD) technique, in order to understand the genetic relationship and to develop species specific markers. The genome relationship dendrogram shows that D. breviaristatum and D. villosum could not be grouped together, indicating that D. breviaristatum was unlikely to be directly derived from D. villosum, while D. breviaristatum was closest to Thinopyrum intermedium, which implied that they might have similar breeding behaviors when introducing their chromatins into wheat. A D. breviaristatum genome specific RAPD product of 1182bp, was cloned and designated as pDb12H. Sequence analysis revealed that pDb12H was strongly homologuos to a long terminal repeat (LTR) Sabrina retrotransposon newly reported in Hordeum. The pDb12H was converted into a PCR based marker, which allows effectively monitoring the D. breviaristatum chromatin introgression into wheat. Fluorescence in situ hybridization (FISH) suggested that pDb12H was specifically hybridized throughout all D. breviaristatum chromosomes arms except for the terminal and centromeric regions, which can be used to characterize wheat -D. breviaristatum chromosome translocation. The genomes repetitive element will also be useful to study gene interactions between the wheat and alien genomes after the polyploidization. SN - 1601-5223 UR - https://www.unboundmedicine.com/medline/citation/17362333/Studies_on_genome_relationship_and_species_specific_PCR_marker_for_Dasypyrum_breviaristatum_in_Triticeae_ L2 - https://doi.org/10.1111/j.2006.0018-0661.01930.x DB - PRIME DP - Unbound Medicine ER -