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Molecular characterization of high molecular weight glutenin subunit allele 1Bx23 in common wheat introduced from hexaploid triticale.
Hereditas. 2006 Dec; 143(2006):159-66.H

Abstract

High molecular weight glutenin subunit (HMW-GS) 1Bx23, an x-type subset encoded by Glu-B1p, which is only distributed in Triticum turgidum, was successfully transferred from hexaploid triticale to common wheat line SY95-71. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) shows that subunit 1Bx23 has a faster mobility than subunit 1Bx7 and 1Bx20, but slower than 1Bx17. Primers designed from the conserved regions in wheat HMW-GS gene promoter and coding sequences were used to amplify the genomic DNA of SY95-71. Total nucleotide sequences of 3426 bp including an open reading frame of 2385 bp and upstream sequence of 1038 bp were obtained. Compared with the reported gene sequences of Glu-B1-1 alleles, including 1Bx7, 1Bx14, 1Bx20 and 1Bx17, the promoter region of the 1Bx23 was displayed close to 1Bx7 and 1Bx17. The deduced amino acid sequence of coding region of 1Bx23 exhibited 34, 30, 20 and 22 amino acid substitutions from that of 1Bx14, 1Bx20, 1Bx7 and 1Bx17, respectively. A phylogenetic tree based on the nucleotide sequence alignment of the Glu-1Bx alleles shows that the 1Bx23 are apparently clustered with 1Bx7 and 1Bx17, and more ancient than 1Bx14 and 1Bx20, suggesting that the evolution speeds are different among Glu-1Bx genes. Additionally, the potential use of wheat line SY95-71 to further screen the quality contribution of unique subunit 1Bx23 is also discussed.

Authors+Show Affiliations

Yang ZJ
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, China. yangzujun@uestc.edu.cn
Li GR
No affiliation info available
Shu HL
No affiliation info available
Liu C
No affiliation info available
Feng J
No affiliation info available
Chang ZJ
No affiliation info available
Ren ZL
No affiliation info available

MeSH

AllelesAmino Acid SequenceBase SequenceCloning, MolecularEdible GrainEvolution, MolecularGenes, PlantGlutensMolecular Sequence DataMolecular WeightPhylogenyPolymerase Chain ReactionProtein SubunitsSequence AlignmentTriticum

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17362350

Citation

Yang, Zu-Jun, et al. "Molecular Characterization of High Molecular Weight Glutenin Subunit Allele 1Bx23 in Common Wheat Introduced From Hexaploid Triticale." Hereditas, vol. 143, no. 2006, 2006, pp. 159-66.
Yang ZJ, Li GR, Shu HL, et al. Molecular characterization of high molecular weight glutenin subunit allele 1Bx23 in common wheat introduced from hexaploid triticale. Hereditas. 2006;143(2006):159-66.
Yang, Z. J., Li, G. R., Shu, H. L., Liu, C., Feng, J., Chang, Z. J., & Ren, Z. L. (2006). Molecular characterization of high molecular weight glutenin subunit allele 1Bx23 in common wheat introduced from hexaploid triticale. Hereditas, 143(2006), 159-66.
Yang ZJ, et al. Molecular Characterization of High Molecular Weight Glutenin Subunit Allele 1Bx23 in Common Wheat Introduced From Hexaploid Triticale. Hereditas. 2006;143(2006):159-66. PubMed PMID: 17362350.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of high molecular weight glutenin subunit allele 1Bx23 in common wheat introduced from hexaploid triticale. AU - Yang,Zu-Jun, AU - Li,Guang-Rong, AU - Shu,Huan-Lin, AU - Liu,Chang, AU - Feng,Juan, AU - Chang,Zhi-Jian, AU - Ren,Zheng-Long, PY - 2007/3/17/pubmed PY - 2007/5/15/medline PY - 2007/3/17/entrez SP - 159 EP - 66 JF - Hereditas JO - Hereditas VL - 143 IS - 2006 N2 - High molecular weight glutenin subunit (HMW-GS) 1Bx23, an x-type subset encoded by Glu-B1p, which is only distributed in Triticum turgidum, was successfully transferred from hexaploid triticale to common wheat line SY95-71. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) shows that subunit 1Bx23 has a faster mobility than subunit 1Bx7 and 1Bx20, but slower than 1Bx17. Primers designed from the conserved regions in wheat HMW-GS gene promoter and coding sequences were used to amplify the genomic DNA of SY95-71. Total nucleotide sequences of 3426 bp including an open reading frame of 2385 bp and upstream sequence of 1038 bp were obtained. Compared with the reported gene sequences of Glu-B1-1 alleles, including 1Bx7, 1Bx14, 1Bx20 and 1Bx17, the promoter region of the 1Bx23 was displayed close to 1Bx7 and 1Bx17. The deduced amino acid sequence of coding region of 1Bx23 exhibited 34, 30, 20 and 22 amino acid substitutions from that of 1Bx14, 1Bx20, 1Bx7 and 1Bx17, respectively. A phylogenetic tree based on the nucleotide sequence alignment of the Glu-1Bx alleles shows that the 1Bx23 are apparently clustered with 1Bx7 and 1Bx17, and more ancient than 1Bx14 and 1Bx20, suggesting that the evolution speeds are different among Glu-1Bx genes. Additionally, the potential use of wheat line SY95-71 to further screen the quality contribution of unique subunit 1Bx23 is also discussed. SN - 1601-5223 UR - https://www.unboundmedicine.com/medline/citation/17362350/Molecular_characterization_of_high_molecular_weight_glutenin_subunit_allele_1Bx23_in_common_wheat_introduced_from_hexaploid_triticale_ L2 - https://doi.org/10.1111/j.2006.0018-0661.01948.x DB - PRIME DP - Unbound Medicine ER -