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Burkholderia Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis.
BMC Microbiol. 2007 Mar 15; 7:19.BM

Abstract

BACKGROUND

The bacterial biothreat agents Burkholderia mallei and Burkholderia pseudomallei are the cause of glanders and melioidosis, respectively. Genomic and epidemiological studies have shown that B. mallei is a recently emerged, host restricted clone of B. pseudomallei.

RESULTS

Using bacteriophage-mediated immunoscreening we identified genes expressed in vivo during experimental equine glanders infection. A family of immunodominant antigens were identified that share protein domain architectures with hemagglutinins and invasins. These have been designated Burkholderia Hep_Hag autotransporter (BuHA) proteins. A total of 110/207 positive clones (53%) of a B. mallei expression library screened with sera from two infected horses belonged to this family. This contrasted with 6/189 positive clones (3%) of a B. pseudomallei expression library screened with serum from 21 patients with culture-proven melioidosis.

CONCLUSION

Members of the BuHA proteins are found in other Gram-negative bacteria and have been shown to have important roles related to virulence. Compared with other bacterial species, the genomes of both B. mallei and B. pseudomallei contain a relative abundance of this family of proteins. The domain structures of these proteins suggest that they function as multimeric surface proteins that modulate interactions of the cell with the host and environment. Their effect on the cellular immune response to B. mallei and their potential as diagnostics for glanders requires further study.

Authors+Show Affiliations

Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. rachaneeporn_t@hotmail.com <rachaneeporn_t@hotmail.com>No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17362501

Citation

Tiyawisutsri, Rachaneeporn, et al. "Burkholderia Hep_Hag Autotransporter (BuHA) Proteins Elicit a Strong Antibody Response During Experimental Glanders but Not Human Melioidosis." BMC Microbiology, vol. 7, 2007, p. 19.
Tiyawisutsri R, Holden MT, Tumapa S, et al. Burkholderia Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis. BMC Microbiol. 2007;7:19.
Tiyawisutsri, R., Holden, M. T., Tumapa, S., Rengpipat, S., Clarke, S. R., Foster, S. J., Nierman, W. C., Day, N. P., & Peacock, S. J. (2007). Burkholderia Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis. BMC Microbiology, 7, 19.
Tiyawisutsri R, et al. Burkholderia Hep_Hag Autotransporter (BuHA) Proteins Elicit a Strong Antibody Response During Experimental Glanders but Not Human Melioidosis. BMC Microbiol. 2007 Mar 15;7:19. PubMed PMID: 17362501.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Burkholderia Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis. AU - Tiyawisutsri,Rachaneeporn, AU - Holden,Matthew T G, AU - Tumapa,Sarinna, AU - Rengpipat,Sirirat, AU - Clarke,Simon R, AU - Foster,Simon J, AU - Nierman,William C, AU - Day,Nicholas P J, AU - Peacock,Sharon J, Y1 - 2007/03/15/ PY - 2006/11/08/received PY - 2007/03/15/accepted PY - 2007/3/17/pubmed PY - 2007/4/27/medline PY - 2007/3/17/entrez SP - 19 EP - 19 JF - BMC microbiology JO - BMC Microbiol VL - 7 N2 - BACKGROUND: The bacterial biothreat agents Burkholderia mallei and Burkholderia pseudomallei are the cause of glanders and melioidosis, respectively. Genomic and epidemiological studies have shown that B. mallei is a recently emerged, host restricted clone of B. pseudomallei. RESULTS: Using bacteriophage-mediated immunoscreening we identified genes expressed in vivo during experimental equine glanders infection. A family of immunodominant antigens were identified that share protein domain architectures with hemagglutinins and invasins. These have been designated Burkholderia Hep_Hag autotransporter (BuHA) proteins. A total of 110/207 positive clones (53%) of a B. mallei expression library screened with sera from two infected horses belonged to this family. This contrasted with 6/189 positive clones (3%) of a B. pseudomallei expression library screened with serum from 21 patients with culture-proven melioidosis. CONCLUSION: Members of the BuHA proteins are found in other Gram-negative bacteria and have been shown to have important roles related to virulence. Compared with other bacterial species, the genomes of both B. mallei and B. pseudomallei contain a relative abundance of this family of proteins. The domain structures of these proteins suggest that they function as multimeric surface proteins that modulate interactions of the cell with the host and environment. Their effect on the cellular immune response to B. mallei and their potential as diagnostics for glanders requires further study. SN - 1471-2180 UR - https://www.unboundmedicine.com/medline/citation/17362501/Burkholderia_Hep_Hag_autotransporter__BuHA__proteins_elicit_a_strong_antibody_response_during_experimental_glanders_but_not_human_melioidosis_ L2 - https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-7-19 DB - PRIME DP - Unbound Medicine ER -