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Expression of lipogenic factors galectin-12, resistin, SREBP-1, and SCD in human sebaceous glands and cultured sebocytes.
J Invest Dermatol. 2007 Jun; 127(6):1309-17.JI

Abstract

The transcription factors CCAAT enhancer-binding protein alpha, beta, and delta, and peroxisome proliferator-activated receptor gamma are known to be crucial to the differentiation of adipocytes and are expressed in sebaceous gland cells. As lipogenesis is key to both adipocyte and sebocyte differentiation we hypothesize that sebocytes follow a similar program of differentiation to adipocytes. We have investigated the expression of known adipogenic factors resistin, galectin-12, sterol response-element-binding protein-1 (SREBP-1) and stearoyl-CoA desaturase in the immortalized sebaceous gland cell line SZ95 and whole skin. Reverse transcriptase-PCR analysis showed the expression of galectin-12, resistin, SREBP-1, and stearoyl-CoA desaturase mRNAs in SZ95 sebocytes. Immunoreactivity was observed for galectin-12 and SREBP-1 in the nuclei and resistin in the cytoplasm of basal sebocytes, and stearoyl CoA desaturase in the cytoplasm of basal and luminal sebocytes of human scalp skin. Expression of galectin-12, resistin, and SREBP-1 in SZ95 sebocytes was confirmed by Western blot analysis. These data provide further evidence that pathways of differentiation in adipocytes and sebocytes could be similar and therefore further understanding of sebaceous gland differentiation and lipogenesis and potential therapies for sebaceous gland disorders may be obtained from our knowledge of adipocyte differentiation.

Authors+Show Affiliations

Centre for Cutaneous Research, Barts and The London, Queen Mary's School of Medicine and Dentistry, London, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17363919

Citation

Harrison, Wesley J., et al. "Expression of Lipogenic Factors Galectin-12, Resistin, SREBP-1, and SCD in Human Sebaceous Glands and Cultured Sebocytes." The Journal of Investigative Dermatology, vol. 127, no. 6, 2007, pp. 1309-17.
Harrison WJ, Bull JJ, Seltmann H, et al. Expression of lipogenic factors galectin-12, resistin, SREBP-1, and SCD in human sebaceous glands and cultured sebocytes. J Invest Dermatol. 2007;127(6):1309-17.
Harrison, W. J., Bull, J. J., Seltmann, H., Zouboulis, C. C., & Philpott, M. P. (2007). Expression of lipogenic factors galectin-12, resistin, SREBP-1, and SCD in human sebaceous glands and cultured sebocytes. The Journal of Investigative Dermatology, 127(6), 1309-17.
Harrison WJ, et al. Expression of Lipogenic Factors Galectin-12, Resistin, SREBP-1, and SCD in Human Sebaceous Glands and Cultured Sebocytes. J Invest Dermatol. 2007;127(6):1309-17. PubMed PMID: 17363919.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of lipogenic factors galectin-12, resistin, SREBP-1, and SCD in human sebaceous glands and cultured sebocytes. AU - Harrison,Wesley J, AU - Bull,Jonathan J, AU - Seltmann,Holger, AU - Zouboulis,Christos C, AU - Philpott,Michael P, Y1 - 2007/03/15/ PY - 2007/3/17/pubmed PY - 2007/6/20/medline PY - 2007/3/17/entrez SP - 1309 EP - 17 JF - The Journal of investigative dermatology JO - J. Invest. Dermatol. VL - 127 IS - 6 N2 - The transcription factors CCAAT enhancer-binding protein alpha, beta, and delta, and peroxisome proliferator-activated receptor gamma are known to be crucial to the differentiation of adipocytes and are expressed in sebaceous gland cells. As lipogenesis is key to both adipocyte and sebocyte differentiation we hypothesize that sebocytes follow a similar program of differentiation to adipocytes. We have investigated the expression of known adipogenic factors resistin, galectin-12, sterol response-element-binding protein-1 (SREBP-1) and stearoyl-CoA desaturase in the immortalized sebaceous gland cell line SZ95 and whole skin. Reverse transcriptase-PCR analysis showed the expression of galectin-12, resistin, SREBP-1, and stearoyl-CoA desaturase mRNAs in SZ95 sebocytes. Immunoreactivity was observed for galectin-12 and SREBP-1 in the nuclei and resistin in the cytoplasm of basal sebocytes, and stearoyl CoA desaturase in the cytoplasm of basal and luminal sebocytes of human scalp skin. Expression of galectin-12, resistin, and SREBP-1 in SZ95 sebocytes was confirmed by Western blot analysis. These data provide further evidence that pathways of differentiation in adipocytes and sebocytes could be similar and therefore further understanding of sebaceous gland differentiation and lipogenesis and potential therapies for sebaceous gland disorders may be obtained from our knowledge of adipocyte differentiation. SN - 1523-1747 UR - https://www.unboundmedicine.com/medline/citation/17363919/Expression_of_lipogenic_factors_galectin_12_resistin_SREBP_1_and_SCD_in_human_sebaceous_glands_and_cultured_sebocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-202X(15)33395-9 DB - PRIME DP - Unbound Medicine ER -