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Molecular cloning and characterization of a cDNA encoding a novel antibacterial peptide, defensin, from the mulberry longicorn beetle, Apriona germari.
DNA Seq 2007; 18(2):160-4DS

Abstract

A full-length cDNA clone with high homology (62% mature peptide sequence identity) to an Acalolepta luxuriosa antibacterial gene, possessing a conserved cysteine-stabilized alphabeta motif, was cloned by screening an Apriona germari cDNA library. This gene (AgCRP) had a total length of 360 bp with an open reading frame of 207 bp, and encoded a predicted peptide of 69 amino acid residues. The mature AgCRP peptide was 27 amino acid residues long and had a cysteine-stabilized alphabeta motif of C...CXXXC...C...CXC consensus sequence, similar to insect defensins. Northern blot analysis revealed that the AgCRP exhibited fat body-specific expression and was up-regulated by wounding, bacterial or fungal challenge.

Authors+Show Affiliations

Joint Laboratory of Shenyang Pharmaceutical University & Dong-A University, Shenyang Pharmaceutical University, Shenyang 110016, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17364828

Citation

Lu, Wei, et al. "Molecular Cloning and Characterization of a cDNA Encoding a Novel Antibacterial Peptide, Defensin, From the Mulberry Longicorn Beetle, Apriona Germari." DNA Sequence : the Journal of DNA Sequencing and Mapping, vol. 18, no. 2, 2007, pp. 160-4.
Lu W, Cui Z, Wang D, et al. Molecular cloning and characterization of a cDNA encoding a novel antibacterial peptide, defensin, from the mulberry longicorn beetle, Apriona germari. DNA Seq. 2007;18(2):160-4.
Lu, W., Cui, Z., Wang, D., Lee, K. S., Park, N. S., Kim, D. H., & Jin, B. R. (2007). Molecular cloning and characterization of a cDNA encoding a novel antibacterial peptide, defensin, from the mulberry longicorn beetle, Apriona germari. DNA Sequence : the Journal of DNA Sequencing and Mapping, 18(2), pp. 160-4.
Lu W, et al. Molecular Cloning and Characterization of a cDNA Encoding a Novel Antibacterial Peptide, Defensin, From the Mulberry Longicorn Beetle, Apriona Germari. DNA Seq. 2007;18(2):160-4. PubMed PMID: 17364828.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning and characterization of a cDNA encoding a novel antibacterial peptide, defensin, from the mulberry longicorn beetle, Apriona germari. AU - Lu,Wei, AU - Cui,Zheng, AU - Wang,Dong, AU - Lee,Kwang Sik, AU - Park,Nam Sook, AU - Kim,Doh Hoon, AU - Jin,Byung Rae, PY - 2007/3/17/pubmed PY - 2007/7/12/medline PY - 2007/3/17/entrez SP - 160 EP - 4 JF - DNA sequence : the journal of DNA sequencing and mapping JO - DNA Seq. VL - 18 IS - 2 N2 - A full-length cDNA clone with high homology (62% mature peptide sequence identity) to an Acalolepta luxuriosa antibacterial gene, possessing a conserved cysteine-stabilized alphabeta motif, was cloned by screening an Apriona germari cDNA library. This gene (AgCRP) had a total length of 360 bp with an open reading frame of 207 bp, and encoded a predicted peptide of 69 amino acid residues. The mature AgCRP peptide was 27 amino acid residues long and had a cysteine-stabilized alphabeta motif of C...CXXXC...C...CXC consensus sequence, similar to insect defensins. Northern blot analysis revealed that the AgCRP exhibited fat body-specific expression and was up-regulated by wounding, bacterial or fungal challenge. SN - 1042-5179 UR - https://www.unboundmedicine.com/medline/citation/17364828/Molecular_cloning_and_characterization_of_a_cDNA_encoding_a_novel_antibacterial_peptide_defensin_from_the_mulberry_longicorn_beetle_Apriona_germari_ L2 - https://medlineplus.gov/antibiotics.html DB - PRIME DP - Unbound Medicine ER -