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Heterogeneities in inflammatory and cytotoxic responses of RAW 264.7 macrophage cell line to urban air coarse, fine, and ultrafine particles from six European sampling campaigns.
Inhal Toxicol. 2007 Mar; 19(3):213-25.IT

Abstract

We investigated the cytotoxic and inflammatory activities of size-segregated particulate samples (particulate matter, PM) from contrasting air pollution situations in Europe. Coarse (PM10-2.5), fine (PM2.5-0.2), and ultrafine (PM0.2) particulate samples were collected with a modified Harvard high-volume cascade impactor (HVCI). Mouse RAW 264.7 macrophages were exposed to the samples for 24 h. Selected inflammatory mediators, nitric oxide (NO) and cytokines (tumor necrosis factor alpha [TNFalpha], interleukin 6 [IL-6], macrophage inflammatory protein-2 [MIP-2]), were measured together with cytotoxicity (MTT test), and analysis of apoptosis and cell cycle (propidium iodide staining). The PM10-2.5 samples had a much higher inflammatory activity than the PM2.5-0.2 and PM0.2 samples, but the PM2.5-0.2 samples showed the largest differences in inflammatory activity, and the PM0.2 samples in cytotoxicity, between the sampling campaigns. The PM2.5-0.2 samples from traffic environments in springtime Barcelona and summertime Athens had the highest inflammatory activities, which may be related to the high photochemical activity in the atmosphere during the sampling campaigns. The PM0.2 sample from wintertime Prague with proven impacts from local coal and biomass combustion had very high cytotoxic and apoptotic activities and caused a distinct cell cycle arrest. Thus, particulate size, sources, and atmospheric transformation processes affect the toxicity profile of urban air particulate matter. These factors may explain some of the heterogeneity observed in particulate exposure-response relationships of human health effects in epidemiological studies.

Authors+Show Affiliations

National Public Health Institute, Department of Environmental Health, Kuopio, Finland. Pasi.Jalava@ktl.fiNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17365026

Citation

Jalava, Pasi I., et al. "Heterogeneities in Inflammatory and Cytotoxic Responses of RAW 264.7 Macrophage Cell Line to Urban Air Coarse, Fine, and Ultrafine Particles From Six European Sampling Campaigns." Inhalation Toxicology, vol. 19, no. 3, 2007, pp. 213-25.
Jalava PI, Salonen RO, Pennanen AS, et al. Heterogeneities in inflammatory and cytotoxic responses of RAW 264.7 macrophage cell line to urban air coarse, fine, and ultrafine particles from six European sampling campaigns. Inhal Toxicol. 2007;19(3):213-25.
Jalava, P. I., Salonen, R. O., Pennanen, A. S., Sillanpää, M., Hälinen, A. I., Happo, M. S., Hillamo, R., Brunekreef, B., Katsouyanni, K., Sunyer, J., & Hirvonen, M. R. (2007). Heterogeneities in inflammatory and cytotoxic responses of RAW 264.7 macrophage cell line to urban air coarse, fine, and ultrafine particles from six European sampling campaigns. Inhalation Toxicology, 19(3), 213-25.
Jalava PI, et al. Heterogeneities in Inflammatory and Cytotoxic Responses of RAW 264.7 Macrophage Cell Line to Urban Air Coarse, Fine, and Ultrafine Particles From Six European Sampling Campaigns. Inhal Toxicol. 2007;19(3):213-25. PubMed PMID: 17365026.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heterogeneities in inflammatory and cytotoxic responses of RAW 264.7 macrophage cell line to urban air coarse, fine, and ultrafine particles from six European sampling campaigns. AU - Jalava,Pasi I, AU - Salonen,Raimo O, AU - Pennanen,Arto S, AU - Sillanpää,Markus, AU - Hälinen,Arja I, AU - Happo,Mikko S, AU - Hillamo,Risto, AU - Brunekreef,Bert, AU - Katsouyanni,Klea, AU - Sunyer,Jordi, AU - Hirvonen,Maija-Riitta, PY - 2007/3/17/pubmed PY - 2007/4/18/medline PY - 2007/3/17/entrez SP - 213 EP - 25 JF - Inhalation toxicology JO - Inhal Toxicol VL - 19 IS - 3 N2 - We investigated the cytotoxic and inflammatory activities of size-segregated particulate samples (particulate matter, PM) from contrasting air pollution situations in Europe. Coarse (PM10-2.5), fine (PM2.5-0.2), and ultrafine (PM0.2) particulate samples were collected with a modified Harvard high-volume cascade impactor (HVCI). Mouse RAW 264.7 macrophages were exposed to the samples for 24 h. Selected inflammatory mediators, nitric oxide (NO) and cytokines (tumor necrosis factor alpha [TNFalpha], interleukin 6 [IL-6], macrophage inflammatory protein-2 [MIP-2]), were measured together with cytotoxicity (MTT test), and analysis of apoptosis and cell cycle (propidium iodide staining). The PM10-2.5 samples had a much higher inflammatory activity than the PM2.5-0.2 and PM0.2 samples, but the PM2.5-0.2 samples showed the largest differences in inflammatory activity, and the PM0.2 samples in cytotoxicity, between the sampling campaigns. The PM2.5-0.2 samples from traffic environments in springtime Barcelona and summertime Athens had the highest inflammatory activities, which may be related to the high photochemical activity in the atmosphere during the sampling campaigns. The PM0.2 sample from wintertime Prague with proven impacts from local coal and biomass combustion had very high cytotoxic and apoptotic activities and caused a distinct cell cycle arrest. Thus, particulate size, sources, and atmospheric transformation processes affect the toxicity profile of urban air particulate matter. These factors may explain some of the heterogeneity observed in particulate exposure-response relationships of human health effects in epidemiological studies. SN - 1091-7691 UR - https://www.unboundmedicine.com/medline/citation/17365026/Heterogeneities_in_inflammatory_and_cytotoxic_responses_of_RAW_264_7_macrophage_cell_line_to_urban_air_coarse_fine_and_ultrafine_particles_from_six_European_sampling_campaigns_ L2 - https://www.tandfonline.com/doi/full/10.1080/08958370601067863 DB - PRIME DP - Unbound Medicine ER -