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Reduction in false antigenemia in the second generation Histoplasma antigen assay.
Med Mycol. 2007 Mar; 45(2):169-71.MM

Abstract

We have previously reported false Histoplasma antigenemia in solid organ transplant patients who received rabbit antithymocyte globulin (ATG). False antigenemia was caused by human anti-rabbit antibody, produced in response to ATG administration. Those specimens were obtained through a research study to assess the development of anti-rabbit antibodies following ATG administration. Here we compare the likelihood of false antigenemia between the original and second generation assays in clinical specimens obtained specifically for the diagnosis of histoplasmosis. Of 12 serum specimens that were falsely positive in the original assay, four (33%) remained positive in the second generation assay. The sensitivity was 98% in urine was and 80% in serum in patients with AIDS complicated by disseminated histoplasmosis. False-positive results were not observed in serum or urine from healthy subjects. Thus, the second generation assay is less susceptible to false antigenemia caused by anti-rabbit antibodies.

Authors+Show Affiliations

MiraVista Diagnostics, Indianapolis, Indiana 46241, USA. jwheat@miravistalabs.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17365653

Citation

Wheat, L Joseph, et al. "Reduction in False Antigenemia in the Second Generation Histoplasma Antigen Assay." Medical Mycology, vol. 45, no. 2, 2007, pp. 169-71.
Wheat LJ, Witt J, Durkin M, et al. Reduction in false antigenemia in the second generation Histoplasma antigen assay. Med Mycol. 2007;45(2):169-71.
Wheat, L. J., Witt, J., Durkin, M., & Connolly, P. (2007). Reduction in false antigenemia in the second generation Histoplasma antigen assay. Medical Mycology, 45(2), 169-71.
Wheat LJ, et al. Reduction in False Antigenemia in the Second Generation Histoplasma Antigen Assay. Med Mycol. 2007;45(2):169-71. PubMed PMID: 17365653.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reduction in false antigenemia in the second generation Histoplasma antigen assay. AU - Wheat,L Joseph, AU - Witt,John,3rd AU - Durkin,Michelle, AU - Connolly,Patricia, PY - 2007/3/17/pubmed PY - 2007/9/5/medline PY - 2007/3/17/entrez SP - 169 EP - 71 JF - Medical mycology JO - Med Mycol VL - 45 IS - 2 N2 - We have previously reported false Histoplasma antigenemia in solid organ transplant patients who received rabbit antithymocyte globulin (ATG). False antigenemia was caused by human anti-rabbit antibody, produced in response to ATG administration. Those specimens were obtained through a research study to assess the development of anti-rabbit antibodies following ATG administration. Here we compare the likelihood of false antigenemia between the original and second generation assays in clinical specimens obtained specifically for the diagnosis of histoplasmosis. Of 12 serum specimens that were falsely positive in the original assay, four (33%) remained positive in the second generation assay. The sensitivity was 98% in urine was and 80% in serum in patients with AIDS complicated by disseminated histoplasmosis. False-positive results were not observed in serum or urine from healthy subjects. Thus, the second generation assay is less susceptible to false antigenemia caused by anti-rabbit antibodies. SN - 1369-3786 UR - https://www.unboundmedicine.com/medline/citation/17365653/Reduction_in_false_antigenemia_in_the_second_generation_Histoplasma_antigen_assay_ L2 - https://academic.oup.com/mmy/article-lookup/doi/10.1080/13693780601185947 DB - PRIME DP - Unbound Medicine ER -