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Determination of the anthraquinones aloe-emodin and aloin-A by liquid chromatography with mass spectrometric and diode array detection.
J AOAC Int. 2007 Jan-Feb; 90(1):28-42.JA

Abstract

Methods using liquid chromatography/mass spectrometry (LC/MS) and LC with diode array detection (DAD) in the UV range (LC/UV) were developed for the determination of low levels of the anthraquinones aloe-emodin and aloin-A (barbaloin) in aloe-based products. The methods were used to analyze several commercial products (liquids, semisolids, and solids) for the 2 anthraquinones. The wavelengths used for quantification of aloin-A, aloe-emodin, and emodin (internal standard) by DAD were 357, 257, and 289 nm, respectively. The on-column sensitivities were 0.25 and 0.05 ng by LC/UV and 0.01 and 0.025 ng by LC/MS for aloin-A and aloe-emodin, respectively. The methods are simple and sensitive and provide reproducible results; therefore, they are suitable for the determination of these anthraquinones in various aloe-based products.

Authors+Show Affiliations

ElSohly Laboratories Inc., 5 Industrial Park Dr, Oxford, MS 38655, USA. elsohly@elsohly.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17373434

Citation

Elsohly, Mahmoud A., et al. "Determination of the Anthraquinones Aloe-emodin and aloin-A By Liquid Chromatography With Mass Spectrometric and Diode Array Detection." Journal of AOAC International, vol. 90, no. 1, 2007, pp. 28-42.
Elsohly MA, Gul W, Avula B, et al. Determination of the anthraquinones aloe-emodin and aloin-A by liquid chromatography with mass spectrometric and diode array detection. J AOAC Int. 2007;90(1):28-42.
Elsohly, M. A., Gul, W., Avula, B., & Khan, I. A. (2007). Determination of the anthraquinones aloe-emodin and aloin-A by liquid chromatography with mass spectrometric and diode array detection. Journal of AOAC International, 90(1), 28-42.
Elsohly MA, et al. Determination of the Anthraquinones Aloe-emodin and aloin-A By Liquid Chromatography With Mass Spectrometric and Diode Array Detection. J AOAC Int. 2007 Jan-Feb;90(1):28-42. PubMed PMID: 17373434.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of the anthraquinones aloe-emodin and aloin-A by liquid chromatography with mass spectrometric and diode array detection. AU - Elsohly,Mahmoud A, AU - Gul,Waseem, AU - Avula,Bharathi, AU - Khan,Ikhlas A, PY - 2007/3/22/pubmed PY - 2007/6/7/medline PY - 2007/3/22/entrez SP - 28 EP - 42 JF - Journal of AOAC International JO - J AOAC Int VL - 90 IS - 1 N2 - Methods using liquid chromatography/mass spectrometry (LC/MS) and LC with diode array detection (DAD) in the UV range (LC/UV) were developed for the determination of low levels of the anthraquinones aloe-emodin and aloin-A (barbaloin) in aloe-based products. The methods were used to analyze several commercial products (liquids, semisolids, and solids) for the 2 anthraquinones. The wavelengths used for quantification of aloin-A, aloe-emodin, and emodin (internal standard) by DAD were 357, 257, and 289 nm, respectively. The on-column sensitivities were 0.25 and 0.05 ng by LC/UV and 0.01 and 0.025 ng by LC/MS for aloin-A and aloe-emodin, respectively. The methods are simple and sensitive and provide reproducible results; therefore, they are suitable for the determination of these anthraquinones in various aloe-based products. SN - 1060-3271 UR - https://www.unboundmedicine.com/medline/citation/17373434/Determination_of_the_anthraquinones_aloe_emodin_and_aloin_A_by_liquid_chromatography_with_mass_spectrometric_and_diode_array_detection_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:17373434 DB - PRIME DP - Unbound Medicine ER -