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Effects of the controlled-released basic fibroblast growth factor from chitosan-gelatin microspheres on human fibroblasts cultured on a chitosan-gelatin scaffold.
Biomacromolecules. 2007 May; 8(5):1446-55.B

Abstract

To provide for prolonged, site-specific delivery of basic fibroblast growth factor (bFGF) to the grafted skin in a convenient manner, biodegradable chitosan-gelatin microspheres containing bFGF were fabricated and incorporated into a porous chitosan-gelatin scaffold in this study. The microspheres are an integral part of the porous three-dimensional scaffolds, and their incorporation does not significantly affect the scaffold porosity and the pore size. The release kinetics of bFGF showed a fast release (23.7%) at the initial phase in the first 2 days, and the ultimate accumulated release was approximately 71.8% by day 14, indicating an extended time course for complete release. Human fibroblasts seeded on chitosan-gelatin scaffolds with and without bFGF-loaded chitosan-gelatin microspheres (bFGF-MS) were incubated in vitro for 2 weeks and showed that, compared to chitosan-gelatin scaffolds alone, the scaffolds with bFGF-MS significantly augmented the proliferation and glycosaminoglycan (GAG) synthesis of human fibroblasts. Moreover, real-time reversed transcribed polymerase chain reaction (RT-PCR) analysis for fibroblast-related extracellular matrix (ECM) gene markers demonstrated that the transcript level of laminin was markedly upregulated by about 9-fold. These results suggest that chitosan-gelatin scaffolds with bFGF-MS possess a promising potential as a tissue engineering scaffold to improve skin regeneration efficacy and to promote vascularization.

Authors+Show Affiliations

Research Institute of Polymer Materials, Tianjin University, Tianjin 300072, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17375950

Citation

Liu, Haifeng, et al. "Effects of the Controlled-released Basic Fibroblast Growth Factor From Chitosan-gelatin Microspheres On Human Fibroblasts Cultured On a Chitosan-gelatin Scaffold." Biomacromolecules, vol. 8, no. 5, 2007, pp. 1446-55.
Liu H, Fan H, Cui Y, et al. Effects of the controlled-released basic fibroblast growth factor from chitosan-gelatin microspheres on human fibroblasts cultured on a chitosan-gelatin scaffold. Biomacromolecules. 2007;8(5):1446-55.
Liu, H., Fan, H., Cui, Y., Chen, Y., Yao, K., & Goh, J. C. (2007). Effects of the controlled-released basic fibroblast growth factor from chitosan-gelatin microspheres on human fibroblasts cultured on a chitosan-gelatin scaffold. Biomacromolecules, 8(5), 1446-55.
Liu H, et al. Effects of the Controlled-released Basic Fibroblast Growth Factor From Chitosan-gelatin Microspheres On Human Fibroblasts Cultured On a Chitosan-gelatin Scaffold. Biomacromolecules. 2007;8(5):1446-55. PubMed PMID: 17375950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of the controlled-released basic fibroblast growth factor from chitosan-gelatin microspheres on human fibroblasts cultured on a chitosan-gelatin scaffold. AU - Liu,Haifeng, AU - Fan,Hongbin, AU - Cui,Yuanlu, AU - Chen,Yiping, AU - Yao,Kangde, AU - Goh,James C H, Y1 - 2007/03/22/ PY - 2007/3/23/pubmed PY - 2007/8/19/medline PY - 2007/3/23/entrez SP - 1446 EP - 55 JF - Biomacromolecules JO - Biomacromolecules VL - 8 IS - 5 N2 - To provide for prolonged, site-specific delivery of basic fibroblast growth factor (bFGF) to the grafted skin in a convenient manner, biodegradable chitosan-gelatin microspheres containing bFGF were fabricated and incorporated into a porous chitosan-gelatin scaffold in this study. The microspheres are an integral part of the porous three-dimensional scaffolds, and their incorporation does not significantly affect the scaffold porosity and the pore size. The release kinetics of bFGF showed a fast release (23.7%) at the initial phase in the first 2 days, and the ultimate accumulated release was approximately 71.8% by day 14, indicating an extended time course for complete release. Human fibroblasts seeded on chitosan-gelatin scaffolds with and without bFGF-loaded chitosan-gelatin microspheres (bFGF-MS) were incubated in vitro for 2 weeks and showed that, compared to chitosan-gelatin scaffolds alone, the scaffolds with bFGF-MS significantly augmented the proliferation and glycosaminoglycan (GAG) synthesis of human fibroblasts. Moreover, real-time reversed transcribed polymerase chain reaction (RT-PCR) analysis for fibroblast-related extracellular matrix (ECM) gene markers demonstrated that the transcript level of laminin was markedly upregulated by about 9-fold. These results suggest that chitosan-gelatin scaffolds with bFGF-MS possess a promising potential as a tissue engineering scaffold to improve skin regeneration efficacy and to promote vascularization. SN - 1525-7797 UR - https://www.unboundmedicine.com/medline/citation/17375950/Effects_of_the_controlled_released_basic_fibroblast_growth_factor_from_chitosan_gelatin_microspheres_on_human_fibroblasts_cultured_on_a_chitosan_gelatin_scaffold_ DB - PRIME DP - Unbound Medicine ER -