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Two-dimensional counter-current chromatography for the preparative separation of prenylflavonoids from Artocarpus altilis.
J Chromatogr A. 2007 Jun 01; 1151(1-2):31-6.JC

Abstract

A two-dimensional counter-current chromatographic system (2D-CCC) for preparative isolation and purification of three prenylflavonoids from Artocarpus altilis is presented. An upright CCC instrument (CCC1, total capacity: 1600 ml) was used as the first dimension. Effluent of interest from CCC1 was collected on-line into a 30 ml sample loop by a laboratory-prepared column-switching interface and introduced into a high-speed CCC instrument (CCC2, total capacity: 210 ml) for the second dimension separation. With this 2D-CCC system and a pair of two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water (5:5:7:3 and 5:5:6.5:3.5, v/v/v/v), which had been selected by high-speed CCC, about a 500 mg amount of the crude extract was separated, yielding 9 mg of compound 1, 28 mg of compound 2 and 78 mg of compound 3. The purities of the three prenylflavonoids were 98.7 (1), 98.3 (2) and 97.2% (3), respectively, as determined by HPLC analysis. Their chemical structures were identified by electrospray ionization MS, (1)H NMR and (13)C NMR.

Authors+Show Affiliations

Department of Chemistry, Zhejiang University, Hangzhou, Zhejiang Province 310027, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17376457

Citation

Lu, Yanbin, et al. "Two-dimensional Counter-current Chromatography for the Preparative Separation of Prenylflavonoids From Artocarpus Altilis." Journal of Chromatography. A, vol. 1151, no. 1-2, 2007, pp. 31-6.
Lu Y, Sun C, Wang Y, et al. Two-dimensional counter-current chromatography for the preparative separation of prenylflavonoids from Artocarpus altilis. J Chromatogr A. 2007;1151(1-2):31-6.
Lu, Y., Sun, C., Wang, Y., & Pan, Y. (2007). Two-dimensional counter-current chromatography for the preparative separation of prenylflavonoids from Artocarpus altilis. Journal of Chromatography. A, 1151(1-2), 31-6.
Lu Y, et al. Two-dimensional Counter-current Chromatography for the Preparative Separation of Prenylflavonoids From Artocarpus Altilis. J Chromatogr A. 2007 Jun 1;1151(1-2):31-6. PubMed PMID: 17376457.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two-dimensional counter-current chromatography for the preparative separation of prenylflavonoids from Artocarpus altilis. AU - Lu,Yanbin, AU - Sun,Cuirong, AU - Wang,Yu, AU - Pan,Yuanjiang, Y1 - 2007/03/03/ PY - 2006/09/10/received PY - 2007/02/26/revised PY - 2007/02/27/accepted PY - 2007/3/23/pubmed PY - 2007/7/6/medline PY - 2007/3/23/entrez SP - 31 EP - 6 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1151 IS - 1-2 N2 - A two-dimensional counter-current chromatographic system (2D-CCC) for preparative isolation and purification of three prenylflavonoids from Artocarpus altilis is presented. An upright CCC instrument (CCC1, total capacity: 1600 ml) was used as the first dimension. Effluent of interest from CCC1 was collected on-line into a 30 ml sample loop by a laboratory-prepared column-switching interface and introduced into a high-speed CCC instrument (CCC2, total capacity: 210 ml) for the second dimension separation. With this 2D-CCC system and a pair of two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water (5:5:7:3 and 5:5:6.5:3.5, v/v/v/v), which had been selected by high-speed CCC, about a 500 mg amount of the crude extract was separated, yielding 9 mg of compound 1, 28 mg of compound 2 and 78 mg of compound 3. The purities of the three prenylflavonoids were 98.7 (1), 98.3 (2) and 97.2% (3), respectively, as determined by HPLC analysis. Their chemical structures were identified by electrospray ionization MS, (1)H NMR and (13)C NMR. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17376457/Two_dimensional_counter_current_chromatography_for_the_preparative_separation_of_prenylflavonoids_from_Artocarpus_altilis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)00431-1 DB - PRIME DP - Unbound Medicine ER -