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Altered response to formalin by L5 spinal nerve ligation in rats: a behavioral and molecular study.
Anesth Analg. 2007 Apr; 104(4):936-43.A&A

Abstract

BACKGROUND

The status of neuropathic pain alters the responsiveness to formalin injection in rats. However, the mechanism by which this alteration occurs is unknown.

METHODS

We used immunocytochemistry to examine the expression of brain-derived neurotrophic factor (BDNF) and calcitonin gene-related peptide (CGRP) in the spinal cord of rats with L5 spinal nerve ligation (SNL)-induced neuropathy, and investigated the expression of c-Fos in the spinal cord after injection of formalin in the hindpaw of rats with SNL.

RESULTS

Four weeks after SNL, the withdrawal threshold was significantly lower in the SNL group than in the sham-operated (sham) group (n = 12 per group, P < 0.05). In the SNL group, expression of BDNF in the L4 (P < 0.05) and L5 (P < 0.01) superficial dorsal horn was significantly decreased compared to that in the sham group. CGRP protein in the L5 but not in the L4, dorsal horn was significantly decreased compared to that in the sham group (P < 0.01). After formalin injection, spontaneous pain responses in the SNL group were significantly decreased compared to those in the sham group (P < 0.05). Immunolabeling for c-Fos was significantly decreased in the L4 and L5 dorsal horn in the SNL group (P < 0.01).

CONCLUSION

Our examination of c-Fos distribution indicates that decreased neuronal activity in the spinal cord in response to inflammatory pain may be important for altering the perception of acute pain. Decreased BDNF expression in response to SNL-induced neuropathy may be involved in this alteration.

Authors+Show Affiliations

Department of Anesthesiology and Resuscitology, Okayama University Medical School, Okayama City 700-8558, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17377110

Citation

Kaku, Ryuji, et al. "Altered Response to Formalin By L5 Spinal Nerve Ligation in Rats: a Behavioral and Molecular Study." Anesthesia and Analgesia, vol. 104, no. 4, 2007, pp. 936-43.
Kaku R, Yokoyama M, Kobayashi H, et al. Altered response to formalin by L5 spinal nerve ligation in rats: a behavioral and molecular study. Anesth Analg. 2007;104(4):936-43.
Kaku, R., Yokoyama, M., Kobayashi, H., Matsuoka, Y., Sato, T., Mizobuchi, S., Itano, Y., & Morita, K. (2007). Altered response to formalin by L5 spinal nerve ligation in rats: a behavioral and molecular study. Anesthesia and Analgesia, 104(4), 936-43.
Kaku R, et al. Altered Response to Formalin By L5 Spinal Nerve Ligation in Rats: a Behavioral and Molecular Study. Anesth Analg. 2007;104(4):936-43. PubMed PMID: 17377110.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Altered response to formalin by L5 spinal nerve ligation in rats: a behavioral and molecular study. AU - Kaku,Ryuji, AU - Yokoyama,Masataka, AU - Kobayashi,Hiroyuki, AU - Matsuoka,Yoshikazu, AU - Sato,Tetsufumi, AU - Mizobuchi,Satoshi, AU - Itano,Yoshitaro, AU - Morita,Kiyoshi, PY - 2007/3/23/pubmed PY - 2007/4/14/medline PY - 2007/3/23/entrez SP - 936 EP - 43 JF - Anesthesia and analgesia JO - Anesth Analg VL - 104 IS - 4 N2 - BACKGROUND: The status of neuropathic pain alters the responsiveness to formalin injection in rats. However, the mechanism by which this alteration occurs is unknown. METHODS: We used immunocytochemistry to examine the expression of brain-derived neurotrophic factor (BDNF) and calcitonin gene-related peptide (CGRP) in the spinal cord of rats with L5 spinal nerve ligation (SNL)-induced neuropathy, and investigated the expression of c-Fos in the spinal cord after injection of formalin in the hindpaw of rats with SNL. RESULTS: Four weeks after SNL, the withdrawal threshold was significantly lower in the SNL group than in the sham-operated (sham) group (n = 12 per group, P < 0.05). In the SNL group, expression of BDNF in the L4 (P < 0.05) and L5 (P < 0.01) superficial dorsal horn was significantly decreased compared to that in the sham group. CGRP protein in the L5 but not in the L4, dorsal horn was significantly decreased compared to that in the sham group (P < 0.01). After formalin injection, spontaneous pain responses in the SNL group were significantly decreased compared to those in the sham group (P < 0.05). Immunolabeling for c-Fos was significantly decreased in the L4 and L5 dorsal horn in the SNL group (P < 0.01). CONCLUSION: Our examination of c-Fos distribution indicates that decreased neuronal activity in the spinal cord in response to inflammatory pain may be important for altering the perception of acute pain. Decreased BDNF expression in response to SNL-induced neuropathy may be involved in this alteration. SN - 1526-7598 UR - https://www.unboundmedicine.com/medline/citation/17377110/Altered_response_to_formalin_by_L5_spinal_nerve_ligation_in_rats:_a_behavioral_and_molecular_study_ L2 - https://doi.org/10.1213/01.ane.0000258762.22607.15 DB - PRIME DP - Unbound Medicine ER -