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On-line coupling of solid-phase microextraction to commercial CE-MS equipment.
Electrophoresis. 2007 May; 28(9):1312-8.E

Abstract

We developed a new interface for the on-line coupling of a fiber for solid-phase microextraction (SPME) with a CE system with an electrospray interface for MS detection. The on-line interface was a modification of a split-flow interface typically used for coupling flow systems to CE. The modification consists of an additional channel closed with a septum that permits the introduction of the fiber in the interface, right at the inlet region of the separation capillary. The interface was designed to be used with commercial fibers. Although commercial fibers can be used directly, we recommend cutting the stationary phase and using fibers with a 2-mm stationary phase. The system was tested for the analysis of tetracycline antibiotic residues, viz., tetracycline, oxytetracycline and doxycycline in soils. The overall accuracy was assessed by regression analysis of spiked samples, showing successful results at a 95% confidence level. The reproducibility, expressed as RSD, was 5.3% and the LOD of tetracycline antibiotics in soil ranged from 2.9 to 3.2 microg/kg.

Authors+Show Affiliations

Santos B
Department of Analytical Chemistry, University of Córdoba, Córdoba, Spain.
Simonet BM
No affiliation info available
Ríos A
No affiliation info available
Valcárcel M
No affiliation info available

MeSH

CalibrationElectrophoresis, CapillaryEquipment DesignOnline SystemsReproducibility of ResultsSensitivity and SpecificitySoilSolid Phase MicroextractionSpectrometry, Mass, Electrospray IonizationSystems IntegrationTetracyclines

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17377942

Citation

Santos, Bricio, et al. "On-line Coupling of Solid-phase Microextraction to Commercial CE-MS Equipment." Electrophoresis, vol. 28, no. 9, 2007, pp. 1312-8.
Santos B, Simonet BM, Ríos A, et al. On-line coupling of solid-phase microextraction to commercial CE-MS equipment. Electrophoresis. 2007;28(9):1312-8.
Santos, B., Simonet, B. M., Ríos, A., & Valcárcel, M. (2007). On-line coupling of solid-phase microextraction to commercial CE-MS equipment. Electrophoresis, 28(9), 1312-8.
Santos B, et al. On-line Coupling of Solid-phase Microextraction to Commercial CE-MS Equipment. Electrophoresis. 2007;28(9):1312-8. PubMed PMID: 17377942.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - On-line coupling of solid-phase microextraction to commercial CE-MS equipment. AU - Santos,Bricio, AU - Simonet,Bartolomé M, AU - Ríos,Angel, AU - Valcárcel,Miguel, PY - 2007/3/23/pubmed PY - 2007/8/10/medline PY - 2007/3/23/entrez SP - 1312 EP - 8 JF - Electrophoresis JO - Electrophoresis VL - 28 IS - 9 N2 - We developed a new interface for the on-line coupling of a fiber for solid-phase microextraction (SPME) with a CE system with an electrospray interface for MS detection. The on-line interface was a modification of a split-flow interface typically used for coupling flow systems to CE. The modification consists of an additional channel closed with a septum that permits the introduction of the fiber in the interface, right at the inlet region of the separation capillary. The interface was designed to be used with commercial fibers. Although commercial fibers can be used directly, we recommend cutting the stationary phase and using fibers with a 2-mm stationary phase. The system was tested for the analysis of tetracycline antibiotic residues, viz., tetracycline, oxytetracycline and doxycycline in soils. The overall accuracy was assessed by regression analysis of spiked samples, showing successful results at a 95% confidence level. The reproducibility, expressed as RSD, was 5.3% and the LOD of tetracycline antibiotics in soil ranged from 2.9 to 3.2 microg/kg. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/17377942/On_line_coupling_of_solid_phase_microextraction_to_commercial_CE_MS_equipment_ L2 - https://doi.org/10.1002/elps.200600429 DB - PRIME DP - Unbound Medicine ER -