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Wound-healing properties of trehalose-stabilized freeze-dried outdated platelets.
Transfusion 2007; 47(4):672-9T

Abstract

BACKGROUND

The wound-healing applications of platelet (PLT)-derived cytokines, proteins, and membranes is accepted but continues to be investigated. In this study, it is demonstrated that stabilized freeze-dried PLTs prepared from outdated PLTs (FDPOs) accelerate wound healing and form tube structures as well as stabilized indated freeze-dried PLTs (FDPIs) and room-temperature fresh PLTs (RT-PLTs).

STUDY DESIGN AND METHODS

Experiments were designed to compare in vitro and in vivo wound-healing properties of FDPI, FDPO, and RT-PLT preparations. The concentration of PLT-derived growth factor (PDGF)-betabeta and transforming growth factor (TGF)-beta1 was determined, and the abilities of FDPIs, FDPOs and RT-PLTs to induce endothelial cell proliferation and promote endothelial cell tube formation (cells formed solid spouts connecting neighboring cells to form tube structures) were observed. Wound-healing characteristics were measured by surgically inducing 1-cm(2), full-thickness wounds on db/db mice (n = 10 per group). The wounds were treated with single or multiple doses of FDPIs and FDPOs. Wound closure rate was determined, and histology samples were evaluated for cellular makeup.

RESULTS

FDPOs retained the same levels of PDGF-betabeta and TGF-beta1 and were able to promote endothelial cell proliferation and tube formation in vitro as well as FDPIs or RT-PLTs. Multiple applications of FDPO accelerated wound closure and enhanced reepithelialization when compared to untreated wounds in db/db mice.

CONCLUSION

FDPOs enhanced wound healing in db/db mice as well as FDPIs and RT-PLTs. Wound closure was obtained 6 days earlier than untreated wounds and histologic examination revealed reduced granulation and increased cellular angiogenesis.

Authors+Show Affiliations

From Adlyfe Inc., and Cellphire Inc., Rockville, Maryland 22101, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17381626

Citation

Sum, Ruth, et al. "Wound-healing Properties of Trehalose-stabilized Freeze-dried Outdated Platelets." Transfusion, vol. 47, no. 4, 2007, pp. 672-9.
Sum R, Hager S, Pietramaggiori G, et al. Wound-healing properties of trehalose-stabilized freeze-dried outdated platelets. Transfusion. 2007;47(4):672-9.
Sum, R., Hager, S., Pietramaggiori, G., Orgill, D. P., Dee, J., Rudolph, A., ... Ho, D. (2007). Wound-healing properties of trehalose-stabilized freeze-dried outdated platelets. Transfusion, 47(4), pp. 672-9.
Sum R, et al. Wound-healing Properties of Trehalose-stabilized Freeze-dried Outdated Platelets. Transfusion. 2007;47(4):672-9. PubMed PMID: 17381626.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Wound-healing properties of trehalose-stabilized freeze-dried outdated platelets. AU - Sum,Ruth, AU - Hager,Sarah, AU - Pietramaggiori,Giorgio, AU - Orgill,Dennis P, AU - Dee,Josh, AU - Rudolph,Alan, AU - Orser,Cindy, AU - Fitzpatrick,G Michael, AU - Ho,David, PY - 2007/3/27/pubmed PY - 2007/9/5/medline PY - 2007/3/27/entrez SP - 672 EP - 9 JF - Transfusion JO - Transfusion VL - 47 IS - 4 N2 - BACKGROUND: The wound-healing applications of platelet (PLT)-derived cytokines, proteins, and membranes is accepted but continues to be investigated. In this study, it is demonstrated that stabilized freeze-dried PLTs prepared from outdated PLTs (FDPOs) accelerate wound healing and form tube structures as well as stabilized indated freeze-dried PLTs (FDPIs) and room-temperature fresh PLTs (RT-PLTs). STUDY DESIGN AND METHODS: Experiments were designed to compare in vitro and in vivo wound-healing properties of FDPI, FDPO, and RT-PLT preparations. The concentration of PLT-derived growth factor (PDGF)-betabeta and transforming growth factor (TGF)-beta1 was determined, and the abilities of FDPIs, FDPOs and RT-PLTs to induce endothelial cell proliferation and promote endothelial cell tube formation (cells formed solid spouts connecting neighboring cells to form tube structures) were observed. Wound-healing characteristics were measured by surgically inducing 1-cm(2), full-thickness wounds on db/db mice (n = 10 per group). The wounds were treated with single or multiple doses of FDPIs and FDPOs. Wound closure rate was determined, and histology samples were evaluated for cellular makeup. RESULTS: FDPOs retained the same levels of PDGF-betabeta and TGF-beta1 and were able to promote endothelial cell proliferation and tube formation in vitro as well as FDPIs or RT-PLTs. Multiple applications of FDPO accelerated wound closure and enhanced reepithelialization when compared to untreated wounds in db/db mice. CONCLUSION: FDPOs enhanced wound healing in db/db mice as well as FDPIs and RT-PLTs. Wound closure was obtained 6 days earlier than untreated wounds and histologic examination revealed reduced granulation and increased cellular angiogenesis. SN - 0041-1132 UR - https://www.unboundmedicine.com/medline/citation/17381626/Wound_healing_properties_of_trehalose_stabilized_freeze_dried_outdated_platelets_ L2 - https://doi.org/10.1111/j.1537-2995.2007.01170.x DB - PRIME DP - Unbound Medicine ER -