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Differential effects of apolipoprotein E3 and E4 on markers of oxidative status in macrophages.
Br J Nutr. 2007 May; 97(5):864-71.BJ

Abstract

ApoE is secreted by macrophages at the lesion site of the atherosclerotic plaque, where it is thought to play a protective role against atherosclerosis independently of its effects on lipid metabolism. Of the three common isoforms for apoE, apoE4 is associated with higher risk of cardiovascular disease (CVD). In vitro studies have shown that recombinant apoE may act as an antioxidant in an isoform-dependent manner (E2 > E3 > E4). The oxidative status of the macrophages plays a key role in the process of atherosclerosis. In the present study the possible differential actions of apoE3 and apoE4 on several parameters of oxidative status were determined in stably transfected murine macrophages (RAW 264-7-apoE3 and - apoE4). No differences between genotypes were observed after peroxide challenge in either protection against cytotoxicity or in cell membrane oxidation, and modest differences were observed in the non-enzymatic antioxidants (glutathione and alpha-tocopherol) in apoE3 v. apoE4 macrophages. Importantly, cells secreting apoE4 showed increased membrane oxidation under basal conditions, and produced more NO and superoxide anion radicals than the apoE3 macrophages after stimulation. The present data suggest that apoE genotype influences the oxidative status of macrophages, and this could partly contribute to the higher CVD risk observed in apoE4 carriers.

Authors+Show Affiliations

Institute of Human Nutrition and Food Science, Christian Albrechts University of Kiel, Hermann-Rodewald-Strasse 6, 24098 Kiel, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17381973

Citation

Jofre-Monseny, Laia, et al. "Differential Effects of Apolipoprotein E3 and E4 On Markers of Oxidative Status in Macrophages." The British Journal of Nutrition, vol. 97, no. 5, 2007, pp. 864-71.
Jofre-Monseny L, de Pascual-Teresa S, Plonka E, et al. Differential effects of apolipoprotein E3 and E4 on markers of oxidative status in macrophages. Br J Nutr. 2007;97(5):864-71.
Jofre-Monseny, L., de Pascual-Teresa, S., Plonka, E., Huebbe, P., Boesch-Saadatmandi, C., Minihane, A. M., & Rimbach, G. (2007). Differential effects of apolipoprotein E3 and E4 on markers of oxidative status in macrophages. The British Journal of Nutrition, 97(5), 864-71.
Jofre-Monseny L, et al. Differential Effects of Apolipoprotein E3 and E4 On Markers of Oxidative Status in Macrophages. Br J Nutr. 2007;97(5):864-71. PubMed PMID: 17381973.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential effects of apolipoprotein E3 and E4 on markers of oxidative status in macrophages. AU - Jofre-Monseny,Laia, AU - de Pascual-Teresa,Sonia, AU - Plonka,Eva, AU - Huebbe,Patricia, AU - Boesch-Saadatmandi,Christine, AU - Minihane,Anne-Marie, AU - Rimbach,Gerald, PY - 2007/3/27/pubmed PY - 2007/12/6/medline PY - 2007/3/27/entrez SP - 864 EP - 71 JF - The British journal of nutrition JO - Br J Nutr VL - 97 IS - 5 N2 - ApoE is secreted by macrophages at the lesion site of the atherosclerotic plaque, where it is thought to play a protective role against atherosclerosis independently of its effects on lipid metabolism. Of the three common isoforms for apoE, apoE4 is associated with higher risk of cardiovascular disease (CVD). In vitro studies have shown that recombinant apoE may act as an antioxidant in an isoform-dependent manner (E2 > E3 > E4). The oxidative status of the macrophages plays a key role in the process of atherosclerosis. In the present study the possible differential actions of apoE3 and apoE4 on several parameters of oxidative status were determined in stably transfected murine macrophages (RAW 264-7-apoE3 and - apoE4). No differences between genotypes were observed after peroxide challenge in either protection against cytotoxicity or in cell membrane oxidation, and modest differences were observed in the non-enzymatic antioxidants (glutathione and alpha-tocopherol) in apoE3 v. apoE4 macrophages. Importantly, cells secreting apoE4 showed increased membrane oxidation under basal conditions, and produced more NO and superoxide anion radicals than the apoE3 macrophages after stimulation. The present data suggest that apoE genotype influences the oxidative status of macrophages, and this could partly contribute to the higher CVD risk observed in apoE4 carriers. SN - 0007-1145 UR - https://www.unboundmedicine.com/medline/citation/17381973/Differential_effects_of_apolipoprotein_E3_and_E4_on_markers_of_oxidative_status_in_macrophages_ L2 - https://www.cambridge.org/core/product/identifier/S0007114507669219/type/journal_article DB - PRIME DP - Unbound Medicine ER -