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Isolation and purification of antioxidative isomeric polyphenols from the roots of Parthenocissus laetevirens by counter-current chromatography.
J Chromatogr A. 2007 Jun 01; 1151(1-2):175-9.JC

Abstract

Upright counter-current chromatography (CCC) with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:2:1:2, v/v/v/v) was applied to the isolation and purification of polyphenols from the roots of Parthenocissus laetevirens. Two cis-trans isomeric resveratrol dimers - quadrangularin A and parthenocissin A - were obtained from the crude sample in a one-step separation, with purities of 95.4 and 97.6%, respectively, as determined by HPLC. The structures of these two compounds were identified by (1)H NMR and (13)C NMR. Furthermore, their antioxidant activities were determined by beta-carotene bleaching assay. The antioxidant activities of quadrangularin A and parthenocissin A were higher than that of vitamin C in this model.

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Authors+Show Affiliations

He S
Department of Chemistry, Zhejiang University, Hangzhou 310027, China.
Lu Y
No affiliation info available
Wu B
No affiliation info available
Pan Y
No affiliation info available

MeSH

AntioxidantsCountercurrent DistributionFlavonoidsMolecular StructurePhenolsPlant RootsPolyphenolsReproducibility of ResultsVitaceaebeta Carotene

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17382336

Citation

He, Shan, et al. "Isolation and Purification of Antioxidative Isomeric Polyphenols From the Roots of Parthenocissus Laetevirens By Counter-current Chromatography." Journal of Chromatography. A, vol. 1151, no. 1-2, 2007, pp. 175-9.
He S, Lu Y, Wu B, et al. Isolation and purification of antioxidative isomeric polyphenols from the roots of Parthenocissus laetevirens by counter-current chromatography. J Chromatogr A. 2007;1151(1-2):175-9.
He, S., Lu, Y., Wu, B., & Pan, Y. (2007). Isolation and purification of antioxidative isomeric polyphenols from the roots of Parthenocissus laetevirens by counter-current chromatography. Journal of Chromatography. A, 1151(1-2), 175-9.
He S, et al. Isolation and Purification of Antioxidative Isomeric Polyphenols From the Roots of Parthenocissus Laetevirens By Counter-current Chromatography. J Chromatogr A. 2007 Jun 1;1151(1-2):175-9. PubMed PMID: 17382336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and purification of antioxidative isomeric polyphenols from the roots of Parthenocissus laetevirens by counter-current chromatography. AU - He,Shan, AU - Lu,Yanbin, AU - Wu,Bin, AU - Pan,Yuanjiang, Y1 - 2007/03/04/ PY - 2006/09/11/received PY - 2007/01/25/revised PY - 2007/02/20/accepted PY - 2007/3/27/pubmed PY - 2007/7/6/medline PY - 2007/3/27/entrez SP - 175 EP - 9 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1151 IS - 1-2 N2 - Upright counter-current chromatography (CCC) with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:2:1:2, v/v/v/v) was applied to the isolation and purification of polyphenols from the roots of Parthenocissus laetevirens. Two cis-trans isomeric resveratrol dimers - quadrangularin A and parthenocissin A - were obtained from the crude sample in a one-step separation, with purities of 95.4 and 97.6%, respectively, as determined by HPLC. The structures of these two compounds were identified by (1)H NMR and (13)C NMR. Furthermore, their antioxidant activities were determined by beta-carotene bleaching assay. The antioxidant activities of quadrangularin A and parthenocissin A were higher than that of vitamin C in this model. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17382336/Isolation_and_purification_of_antioxidative_isomeric_polyphenols_from_the_roots_of_Parthenocissus_laetevirens_by_counter_current_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)00369-X DB - PRIME DP - Unbound Medicine ER -