Tags

Type your tag names separated by a space and hit enter

Antioxidant responses and lipid peroxidation following intranasal 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administration in rats: increased susceptibility of olfactory bulb.
Life Sci. 2007 Apr 24; 80(20):1906-14.LS

Abstract

We evaluated an alternative method to investigate a possible involvement of environmental toxins in the pathology of Parkinson's disease (PD). There is considerable evidence supporting the role of oxidative stress in the toxicity of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), a neurotoxin largely used to modeling PD in primates and rodents. We have recently demonstrated that rats treated with intranasal (i.n.) infusion of MPTP suffer from progressive signs of PD that are correlated with time-dependent degeneration in dopaminergic neurons. In the present study, we investigated the time-dependent (2 h to 7 days) effect of a single i.n. administration of MPTP (0.1 mg/nostril) on the glutathione-related antioxidant status and lipid peroxidation (TBARS) in the adult Wistar rat brain. The effects were more pronounced in the olfactory bulb at 6 h after i.n. MPTP administration, as indicated by an increase in TBARS and total glutathione (GSH-t) levels, and also in the gamma-glutamyl transpeptidase (GGT) activity. Increased levels of TBARS, GSH-t and GGT activity were also observed at 6 h post-MPTP infusion in some structures (e.g. striatum, hippocampus and prefrontal cortex). No difference regarding glutathione reductase activity was observed in any of the brain structures analyzed, while a marked decrease in glutathione peroxidase activity was specifically observed in the substantia nigra 7 days after MPTP treatment. These results demonstrate that a single i.n. infusion of MPTP in rats induces significant alterations in the brain antioxidant status and lipid peroxidation, reinforcing the notion that the olfactory system represents a particularly sensitive route for the transport of neurotoxins into the central nervous system that may be related to the etiology of PD.

Authors+Show Affiliations

Departamento de Ciências Fisiológicas, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, 88049-900, Florianópolis-SC, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17382353

Citation

Franco, Jeferson, et al. "Antioxidant Responses and Lipid Peroxidation Following Intranasal 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) Administration in Rats: Increased Susceptibility of Olfactory Bulb." Life Sciences, vol. 80, no. 20, 2007, pp. 1906-14.
Franco J, Prediger RD, Pandolfo P, et al. Antioxidant responses and lipid peroxidation following intranasal 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administration in rats: increased susceptibility of olfactory bulb. Life Sci. 2007;80(20):1906-14.
Franco, J., Prediger, R. D., Pandolfo, P., Takahashi, R. N., Farina, M., & Dafre, A. L. (2007). Antioxidant responses and lipid peroxidation following intranasal 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administration in rats: increased susceptibility of olfactory bulb. Life Sciences, 80(20), 1906-14.
Franco J, et al. Antioxidant Responses and Lipid Peroxidation Following Intranasal 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) Administration in Rats: Increased Susceptibility of Olfactory Bulb. Life Sci. 2007 Apr 24;80(20):1906-14. PubMed PMID: 17382353.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant responses and lipid peroxidation following intranasal 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administration in rats: increased susceptibility of olfactory bulb. AU - Franco,Jeferson, AU - Prediger,Rui D S, AU - Pandolfo,Pablo, AU - Takahashi,Reinaldo N, AU - Farina,Marcelo, AU - Dafre,Alcir L, Y1 - 2007/02/24/ PY - 2006/07/10/received PY - 2006/12/07/revised PY - 2007/02/19/accepted PY - 2007/3/27/pubmed PY - 2007/6/26/medline PY - 2007/3/27/entrez SP - 1906 EP - 14 JF - Life sciences JO - Life Sci VL - 80 IS - 20 N2 - We evaluated an alternative method to investigate a possible involvement of environmental toxins in the pathology of Parkinson's disease (PD). There is considerable evidence supporting the role of oxidative stress in the toxicity of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), a neurotoxin largely used to modeling PD in primates and rodents. We have recently demonstrated that rats treated with intranasal (i.n.) infusion of MPTP suffer from progressive signs of PD that are correlated with time-dependent degeneration in dopaminergic neurons. In the present study, we investigated the time-dependent (2 h to 7 days) effect of a single i.n. administration of MPTP (0.1 mg/nostril) on the glutathione-related antioxidant status and lipid peroxidation (TBARS) in the adult Wistar rat brain. The effects were more pronounced in the olfactory bulb at 6 h after i.n. MPTP administration, as indicated by an increase in TBARS and total glutathione (GSH-t) levels, and also in the gamma-glutamyl transpeptidase (GGT) activity. Increased levels of TBARS, GSH-t and GGT activity were also observed at 6 h post-MPTP infusion in some structures (e.g. striatum, hippocampus and prefrontal cortex). No difference regarding glutathione reductase activity was observed in any of the brain structures analyzed, while a marked decrease in glutathione peroxidase activity was specifically observed in the substantia nigra 7 days after MPTP treatment. These results demonstrate that a single i.n. infusion of MPTP in rats induces significant alterations in the brain antioxidant status and lipid peroxidation, reinforcing the notion that the olfactory system represents a particularly sensitive route for the transport of neurotoxins into the central nervous system that may be related to the etiology of PD. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/17382353/Antioxidant_responses_and_lipid_peroxidation_following_intranasal_1_methyl_4_phenyl_1236_tetrahydropyridine__MPTP__administration_in_rats:_increased_susceptibility_of_olfactory_bulb_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(07)00184-1 DB - PRIME DP - Unbound Medicine ER -