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Evaluation of hemoglobin of blood donors deferred by the copper sulphate method for hemoglobin estimation.
Transfus Apher Sci. 2007 Apr; 36(2):143-8.TA

Abstract

INTRODUCTION

The copper sulphate (CuSO4) specific gravity test for Hb screening tends to give inappropriate failures. This prompted us to compare it with alternate screening methods.

AIM

To study the impact of inaccuracy of CuSO4 method on donor deferral.

METHODS

Capillary and venous blood samples of 400 potential blood donors failing the primary Hb screening using appropriately standardized CuSO4 test (specific gravity 1.053) were tested by Hemocue photometer, the Hb colour scale, Cyanmethemoglobin method as well as the automated hematology analyser, which was considered as the standard reference method.

RESULTS

One hundred and sixteen donors (29%) who failed the CuSO4 test had true Hb levels >12.5 g/dl. The Hb levels of 131 (32.8%) deferred donors were between 12 and 12.5 g/dl. The sensitivity of Hemocue, Hb colour scale and Cyanmethemoglobin was 99%, 97% and 96% and their specificity was 45%, 93% and 46%, respectively. The positive predictive values (PPV) of Hemocue and Cyanmethemoglobin methods were low (43% and 44%, respectively) but their negative predictive values (NPV) were high (99%, and 97%, respectively). The Hb colour scale had an overall best performance with a PPV of 96% and NPV of 95%.

CONCLUSION

The Hemoglobin colour scale which is inexpensive, convenient for field testing and has the overall best performance, is the most suitable for donor Hb screening. Since its readability is 12 g/dl, lowering the donor Hb threshold to 12 g/dl should be actively considered.

Authors+Show Affiliations

Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Department of Transfusion Medicine, Tata Memorial Centre, Sector 22 Kharghar, Navi Mumbai 410208, India. sawantrb72@rediffmail.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17382593

Citation

Sawant, R B., et al. "Evaluation of Hemoglobin of Blood Donors Deferred By the Copper Sulphate Method for Hemoglobin Estimation." Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis, vol. 36, no. 2, 2007, pp. 143-8.
Sawant RB, Bharucha ZS, Rajadhyaksha SB. Evaluation of hemoglobin of blood donors deferred by the copper sulphate method for hemoglobin estimation. Transfus Apher Sci. 2007;36(2):143-8.
Sawant, R. B., Bharucha, Z. S., & Rajadhyaksha, S. B. (2007). Evaluation of hemoglobin of blood donors deferred by the copper sulphate method for hemoglobin estimation. Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis, 36(2), 143-8.
Sawant RB, Bharucha ZS, Rajadhyaksha SB. Evaluation of Hemoglobin of Blood Donors Deferred By the Copper Sulphate Method for Hemoglobin Estimation. Transfus Apher Sci. 2007;36(2):143-8. PubMed PMID: 17382593.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of hemoglobin of blood donors deferred by the copper sulphate method for hemoglobin estimation. AU - Sawant,R B, AU - Bharucha,Z S, AU - Rajadhyaksha,S B, Y1 - 2007/03/26/ PY - 2006/07/20/received PY - 2006/11/21/revised PY - 2006/11/21/accepted PY - 2007/3/27/pubmed PY - 2007/9/13/medline PY - 2007/3/27/entrez SP - 143 EP - 8 JF - Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis JO - Transfus. Apher. Sci. VL - 36 IS - 2 N2 - INTRODUCTION: The copper sulphate (CuSO4) specific gravity test for Hb screening tends to give inappropriate failures. This prompted us to compare it with alternate screening methods. AIM: To study the impact of inaccuracy of CuSO4 method on donor deferral. METHODS: Capillary and venous blood samples of 400 potential blood donors failing the primary Hb screening using appropriately standardized CuSO4 test (specific gravity 1.053) were tested by Hemocue photometer, the Hb colour scale, Cyanmethemoglobin method as well as the automated hematology analyser, which was considered as the standard reference method. RESULTS: One hundred and sixteen donors (29%) who failed the CuSO4 test had true Hb levels >12.5 g/dl. The Hb levels of 131 (32.8%) deferred donors were between 12 and 12.5 g/dl. The sensitivity of Hemocue, Hb colour scale and Cyanmethemoglobin was 99%, 97% and 96% and their specificity was 45%, 93% and 46%, respectively. The positive predictive values (PPV) of Hemocue and Cyanmethemoglobin methods were low (43% and 44%, respectively) but their negative predictive values (NPV) were high (99%, and 97%, respectively). The Hb colour scale had an overall best performance with a PPV of 96% and NPV of 95%. CONCLUSION: The Hemoglobin colour scale which is inexpensive, convenient for field testing and has the overall best performance, is the most suitable for donor Hb screening. Since its readability is 12 g/dl, lowering the donor Hb threshold to 12 g/dl should be actively considered. SN - 1473-0502 UR - https://www.unboundmedicine.com/medline/citation/17382593/Evaluation_of_hemoglobin_of_blood_donors_deferred_by_the_copper_sulphate_method_for_hemoglobin_estimation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1473-0502(07)00022-5 DB - PRIME DP - Unbound Medicine ER -