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Method development for the determination of coumarin compounds by capillary electrophoresis with indirect laser-induced fluorescence detection.
J Chromatogr A. 2007 Apr 27; 1148(1):108-14.JC

Abstract

A capillary zone electrophoresis (CZE) with indirect laser-induced fluorescence detection (ILIFD) method is described for the simultaneous determination of esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside. The baseline separation was achieved within 5 min with running buffer (pH 9.4) composed of 5mM borate, 20% methanol (v/v) as organic modifier, 10(-7)M fluorescein sodium as background fluorophore and 20 kV of applied voltage at 30 degrees C of cartridge temperature. Good linearity relationships (correlation coefficients >0.9900) between the second-order derivative peak-heights (RFU) and concentrations of the analytes (mol L(-1)) were obtained. The detection limits for all analytes in second-order derivative electrophoregrams were in the range of 3.8-15 microM. The RSD data of intra-day for migration times and second-order derivative peak-height were less than 0.95 and 5.02%, respectively. This developed method was applied to the analysis of the courmin compounds in herb plants with recoveries in the range of 94.7-102.1%. In this work, although the detection sensitivity was lower than that of direct LIF, yet the method would extend the application range of LIF detection.

Authors+Show Affiliations

Department of Chemistry, Lanzhou University, Lanzhou 730000, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17383667

Citation

Wang, Weiping, et al. "Method Development for the Determination of Coumarin Compounds By Capillary Electrophoresis With Indirect Laser-induced Fluorescence Detection." Journal of Chromatography. A, vol. 1148, no. 1, 2007, pp. 108-14.
Wang W, Tang J, Wang S, et al. Method development for the determination of coumarin compounds by capillary electrophoresis with indirect laser-induced fluorescence detection. J Chromatogr A. 2007;1148(1):108-14.
Wang, W., Tang, J., Wang, S., Zhou, L., & Hu, Z. (2007). Method development for the determination of coumarin compounds by capillary electrophoresis with indirect laser-induced fluorescence detection. Journal of Chromatography. A, 1148(1), 108-14.
Wang W, et al. Method Development for the Determination of Coumarin Compounds By Capillary Electrophoresis With Indirect Laser-induced Fluorescence Detection. J Chromatogr A. 2007 Apr 27;1148(1):108-14. PubMed PMID: 17383667.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Method development for the determination of coumarin compounds by capillary electrophoresis with indirect laser-induced fluorescence detection. AU - Wang,Weiping, AU - Tang,Jianghong, AU - Wang,Shumin, AU - Zhou,Lei, AU - Hu,Zhide, Y1 - 2007/03/26/ PY - 2006/07/11/received PY - 2006/09/20/revised PY - 2006/09/25/accepted PY - 2007/3/27/pubmed PY - 2007/6/15/medline PY - 2007/3/27/entrez SP - 108 EP - 14 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1148 IS - 1 N2 - A capillary zone electrophoresis (CZE) with indirect laser-induced fluorescence detection (ILIFD) method is described for the simultaneous determination of esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside. The baseline separation was achieved within 5 min with running buffer (pH 9.4) composed of 5mM borate, 20% methanol (v/v) as organic modifier, 10(-7)M fluorescein sodium as background fluorophore and 20 kV of applied voltage at 30 degrees C of cartridge temperature. Good linearity relationships (correlation coefficients >0.9900) between the second-order derivative peak-heights (RFU) and concentrations of the analytes (mol L(-1)) were obtained. The detection limits for all analytes in second-order derivative electrophoregrams were in the range of 3.8-15 microM. The RSD data of intra-day for migration times and second-order derivative peak-height were less than 0.95 and 5.02%, respectively. This developed method was applied to the analysis of the courmin compounds in herb plants with recoveries in the range of 94.7-102.1%. In this work, although the detection sensitivity was lower than that of direct LIF, yet the method would extend the application range of LIF detection. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17383667/Method_development_for_the_determination_of_coumarin_compounds_by_capillary_electrophoresis_with_indirect_laser_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(06)01860-7 DB - PRIME DP - Unbound Medicine ER -