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Determination of catecholamines and serotonin by micellar electrokinetic chromatography with laser-induced fluorescence detection.
Biomed Chromatogr. 2007 Jul; 21(7):708-15.BC

Abstract

6-Oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl) fluorescein, a new synthesized fluorescent reagent, was established for the first time as a label for the sensitive analysis of catecholamines (CAs) and serotonin (5-HT) by micellar electrokinetic chromatography (MEKC) with laser-induced fluorescence detection. After careful study on the derivatization conditions such as pH value, reagent concentration, temperature and reaction time, the labeling reaction was accomplished as quickly as 7 min with stable yield. The separation parameters for the CAs and 5-HT were also optimized in detail. The derivatives were baseline separated in a running buffer containing 30 mM boric acid and 15 mM sodium dodeculsulfate at pH 9.0. The detection limits ranged from 5 x 10(-10) to 2 x 10(-9) M (signal-to-noise ratio = 3). The rapid and sensitive method was also applied to the determination of the CAs and 5-HT of urine samples.

Authors+Show Affiliations

Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China. lwcao@126.com

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17385806

Citation

Cao, Li-Wei. "Determination of Catecholamines and Serotonin By Micellar Electrokinetic Chromatography With Laser-induced Fluorescence Detection." Biomedical Chromatography : BMC, vol. 21, no. 7, 2007, pp. 708-15.
Cao LW. Determination of catecholamines and serotonin by micellar electrokinetic chromatography with laser-induced fluorescence detection. Biomed Chromatogr. 2007;21(7):708-15.
Cao, L. W. (2007). Determination of catecholamines and serotonin by micellar electrokinetic chromatography with laser-induced fluorescence detection. Biomedical Chromatography : BMC, 21(7), 708-15.
Cao LW. Determination of Catecholamines and Serotonin By Micellar Electrokinetic Chromatography With Laser-induced Fluorescence Detection. Biomed Chromatogr. 2007;21(7):708-15. PubMed PMID: 17385806.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of catecholamines and serotonin by micellar electrokinetic chromatography with laser-induced fluorescence detection. A1 - Cao,Li-Wei, PY - 2007/3/28/pubmed PY - 2007/10/20/medline PY - 2007/3/28/entrez SP - 708 EP - 15 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 21 IS - 7 N2 - 6-Oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl) fluorescein, a new synthesized fluorescent reagent, was established for the first time as a label for the sensitive analysis of catecholamines (CAs) and serotonin (5-HT) by micellar electrokinetic chromatography (MEKC) with laser-induced fluorescence detection. After careful study on the derivatization conditions such as pH value, reagent concentration, temperature and reaction time, the labeling reaction was accomplished as quickly as 7 min with stable yield. The separation parameters for the CAs and 5-HT were also optimized in detail. The derivatives were baseline separated in a running buffer containing 30 mM boric acid and 15 mM sodium dodeculsulfate at pH 9.0. The detection limits ranged from 5 x 10(-10) to 2 x 10(-9) M (signal-to-noise ratio = 3). The rapid and sensitive method was also applied to the determination of the CAs and 5-HT of urine samples. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/17385806/Determination_of_catecholamines_and_serotonin_by_micellar_electrokinetic_chromatography_with_laser_induced_fluorescence_detection_ L2 - https://doi.org/10.1002/bmc.809 DB - PRIME DP - Unbound Medicine ER -