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Presence of virgin olive oil phenolic metabolites in human low density lipoprotein fraction: determination by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry.
Anal Chim Acta. 2007 Feb 05; 583(2):402-10.AC

Abstract

The biological benefits of olive oil in preventing the oxidation of low density lipoprotein (LDL) would seem to be linked to its high monounsaturated fatty acid contents, but also to its respective phenolic compounds contents. One prerequisite to assess the in vivo physiological significance of phenolic compounds is to determine their presence in human LDL following the ingestion of virgin olive oil. In this work, olive oil phenolic metabolites were identified using high-performance liquid chromatography in tandem with electrospray mass spectrometry (HPLC-ESI-MS/MS) detection, after solid phase extraction (SPE). Quantitative methods were developed in carrying out linearity, precision, sensitivity and recovery tests. The results from two methods of LDL separation were compared and shorter LDL isolation procedure showed a better recovery for antioxidants compounds in LDL. The metabolites identified in LDL were: hydroxytyrosol monoglucuronide, hydroxytyrosol monosulfate, tyrosol glucuronide, tyrosol sulfate and homovanillic acid sulfate. The fact that olive oil phenolic metabolites are able to bind LDL strengthens claims that these compounds act as in vivo antioxidants.

Authors+Show Affiliations

Department of Nutrition and Food Science, Reference Center in Food Technology, Faculty of Pharmacy, University of Barcelona, Avda. Joan XXIII s/n, 08028 Barcelona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17386573

Citation

de la Torre-Carbot, Karina, et al. "Presence of Virgin Olive Oil Phenolic Metabolites in Human Low Density Lipoprotein Fraction: Determination By High-performance Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry." Analytica Chimica Acta, vol. 583, no. 2, 2007, pp. 402-10.
de la Torre-Carbot K, Chávez-Servín JL, Jaúregui O, et al. Presence of virgin olive oil phenolic metabolites in human low density lipoprotein fraction: determination by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. Anal Chim Acta. 2007;583(2):402-10.
de la Torre-Carbot, K., Chávez-Servín, J. L., Jaúregui, O., Castellote, A. I., Lamuela-Raventós, R. M., Fitó, M., Covas, M. I., Muñoz-Aguayo, D., & López-Sabater, M. C. (2007). Presence of virgin olive oil phenolic metabolites in human low density lipoprotein fraction: determination by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. Analytica Chimica Acta, 583(2), 402-10.
de la Torre-Carbot K, et al. Presence of Virgin Olive Oil Phenolic Metabolites in Human Low Density Lipoprotein Fraction: Determination By High-performance Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry. Anal Chim Acta. 2007 Feb 5;583(2):402-10. PubMed PMID: 17386573.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Presence of virgin olive oil phenolic metabolites in human low density lipoprotein fraction: determination by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. AU - de la Torre-Carbot,Karina, AU - Chávez-Servín,Jorge L, AU - Jaúregui,Olga, AU - Castellote,Ana I, AU - Lamuela-Raventós,Rosa M, AU - Fitó,Montserrat, AU - Covas,María-Isabel, AU - Muñoz-Aguayo,Daniel, AU - López-Sabater,M Carmen, Y1 - 2006/11/02/ PY - 2006/08/03/received PY - 2006/10/09/revised PY - 2006/10/09/accepted PY - 2007/3/28/pubmed PY - 2007/5/11/medline PY - 2007/3/28/entrez SP - 402 EP - 10 JF - Analytica chimica acta JO - Anal Chim Acta VL - 583 IS - 2 N2 - The biological benefits of olive oil in preventing the oxidation of low density lipoprotein (LDL) would seem to be linked to its high monounsaturated fatty acid contents, but also to its respective phenolic compounds contents. One prerequisite to assess the in vivo physiological significance of phenolic compounds is to determine their presence in human LDL following the ingestion of virgin olive oil. In this work, olive oil phenolic metabolites were identified using high-performance liquid chromatography in tandem with electrospray mass spectrometry (HPLC-ESI-MS/MS) detection, after solid phase extraction (SPE). Quantitative methods were developed in carrying out linearity, precision, sensitivity and recovery tests. The results from two methods of LDL separation were compared and shorter LDL isolation procedure showed a better recovery for antioxidants compounds in LDL. The metabolites identified in LDL were: hydroxytyrosol monoglucuronide, hydroxytyrosol monosulfate, tyrosol glucuronide, tyrosol sulfate and homovanillic acid sulfate. The fact that olive oil phenolic metabolites are able to bind LDL strengthens claims that these compounds act as in vivo antioxidants. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/17386573/Presence_of_virgin_olive_oil_phenolic_metabolites_in_human_low_density_lipoprotein_fraction:_determination_by_high_performance_liquid_chromatography_electrospray_ionization_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(06)02028-9 DB - PRIME DP - Unbound Medicine ER -