Tags

Type your tag names separated by a space and hit enter

Improving the sensitivity of the determination of ceftiofur by capillary electrophoresis in environmental water samples: in-line solid phase extraction and sample stacking techniques.
Anal Chim Acta. 2007 Mar 28; 587(2):208-15.AC

Abstract

This paper describes two different approaches for increasing the sensitivity for the analysis of ceftiofur by capillary electrophoresis (CE). Two different techniques based on the introduction of an enlarged volume of sample, namely large volume sample stacking (LVSS) and in-line solid phase extraction (SPE) were studied and compared. LVSS allowed the on-column electrophoretic preconcentration of ceftiofur without modification of the separation capillary. In-line SPE-CE was developed by using a home-made microcartridge that was filled with a reversed-phase sorbent (C(18)). The microcartridge was coupled in-line near the inlet of the separation capillary. LVSS and in-line SPE-CE allowed automated operation and improved sensitivity for the analysis of ceftiofur with respect to conventional CE. When environmental water samples were analyzed, an additional pretreatment step based on off-line SPE was necessary in both cases to further decrease the detection limits. In terms of sensitivity for the determination of ceftiofur in river water samples, the combination of off-line SPE with in-line SPE-CE was found the most sensitive with a detection limit of 10 ng L(-1), whereas the method based on the use of off-line SPE with LVSS presented a detection limit of 100 ng L(-1).

Links

Publisher Full Text

Authors+Show Affiliations

Puig P
Department of Analytical and Organic Chemistry, Rovira i Virgili University, Marcel li Domingo, E-43007 Tarragona, Spain.
Borrull F
No affiliation info available
Calull M
No affiliation info available
Benavente F
No affiliation info available
Sanz-Nebot V
No affiliation info available
Barbosa J
No affiliation info available
Aguilar C
No affiliation info available

MeSH

AutomationCalibrationCephalosporinsElectrodesElectrophoresis, CapillaryEnvironmentEnvironmental MonitoringFresh WaterModels, ChemicalRiversSensitivity and SpecificitySolid Phase ExtractionWater Pollutants, Chemical

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17386775

Citation

Puig, Patricia, et al. "Improving the Sensitivity of the Determination of Ceftiofur By Capillary Electrophoresis in Environmental Water Samples: In-line Solid Phase Extraction and Sample Stacking Techniques." Analytica Chimica Acta, vol. 587, no. 2, 2007, pp. 208-15.
Puig P, Borrull F, Calull M, et al. Improving the sensitivity of the determination of ceftiofur by capillary electrophoresis in environmental water samples: in-line solid phase extraction and sample stacking techniques. Anal Chim Acta. 2007;587(2):208-15.
Puig, P., Borrull, F., Calull, M., Benavente, F., Sanz-Nebot, V., Barbosa, J., & Aguilar, C. (2007). Improving the sensitivity of the determination of ceftiofur by capillary electrophoresis in environmental water samples: in-line solid phase extraction and sample stacking techniques. Analytica Chimica Acta, 587(2), 208-15.
Puig P, et al. Improving the Sensitivity of the Determination of Ceftiofur By Capillary Electrophoresis in Environmental Water Samples: In-line Solid Phase Extraction and Sample Stacking Techniques. Anal Chim Acta. 2007 Mar 28;587(2):208-15. PubMed PMID: 17386775.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improving the sensitivity of the determination of ceftiofur by capillary electrophoresis in environmental water samples: in-line solid phase extraction and sample stacking techniques. AU - Puig,Patricia, AU - Borrull,Francesc, AU - Calull,Marta, AU - Benavente,Fernando, AU - Sanz-Nebot,Victoria, AU - Barbosa,José, AU - Aguilar,Carme, Y1 - 2007/01/21/ PY - 2006/11/16/received PY - 2007/01/16/revised PY - 2007/01/18/accepted PY - 2007/3/28/pubmed PY - 2007/5/12/medline PY - 2007/3/28/entrez SP - 208 EP - 15 JF - Analytica chimica acta JO - Anal Chim Acta VL - 587 IS - 2 N2 - This paper describes two different approaches for increasing the sensitivity for the analysis of ceftiofur by capillary electrophoresis (CE). Two different techniques based on the introduction of an enlarged volume of sample, namely large volume sample stacking (LVSS) and in-line solid phase extraction (SPE) were studied and compared. LVSS allowed the on-column electrophoretic preconcentration of ceftiofur without modification of the separation capillary. In-line SPE-CE was developed by using a home-made microcartridge that was filled with a reversed-phase sorbent (C(18)). The microcartridge was coupled in-line near the inlet of the separation capillary. LVSS and in-line SPE-CE allowed automated operation and improved sensitivity for the analysis of ceftiofur with respect to conventional CE. When environmental water samples were analyzed, an additional pretreatment step based on off-line SPE was necessary in both cases to further decrease the detection limits. In terms of sensitivity for the determination of ceftiofur in river water samples, the combination of off-line SPE with in-line SPE-CE was found the most sensitive with a detection limit of 10 ng L(-1), whereas the method based on the use of off-line SPE with LVSS presented a detection limit of 100 ng L(-1). SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/17386775/Improving_the_sensitivity_of_the_determination_of_ceftiofur_by_capillary_electrophoresis_in_environmental_water_samples:_in_line_solid_phase_extraction_and_sample_stacking_techniques_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(07)00181-X DB - PRIME DP - Unbound Medicine ER -