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N-Acetyltransferase Mpr1 confers ethanol tolerance on Saccharomyces cerevisiae by reducing reactive oxygen species.
Appl Microbiol Biotechnol. 2007 Jul; 75(6):1343-51.AM

Abstract

N-Acetyltransferase Mpr1 of Saccharomyces cerevisiae can reduce intracellular oxidation levels and protect yeast cells under oxidative stress, including H(2)O(2), heat-shock, or freeze-thaw treatment. Unlike many antioxidant enzyme genes induced in response to oxidative stress, the MPR1 gene seems to be constitutively expressed in yeast cells. Based on a recent report that ethanol toxicity is correlated with the production of reactive oxygen species (ROS), we examined here the role of Mpr1 under ethanol stress conditions. The null mutant of the MPR1 and MPR2 genes showed hypersensitivity to ethanol stress, and the expression of the MPR1 gene conferred stress tolerance. We also found that yeast cells exhibited increased ROS levels during exposure to ethanol stress, and that Mpr1 protects yeast cells from ethanol stress by reducing intracellular ROS levels. When the MPR1 gene was overexpressed in antioxidant enzyme-deficient mutants, increased resistance to H(2)O(2) or heat shock was observed in cells lacking the CTA1, CTT1, or GPX1 gene encoding catalase A, catalase T, or glutathione peroxidase, respectively. These results suggest that Mpr1 might compensate the function of enzymes that detoxify H(2)O(2). Hence, Mpr1 has promising potential for the breeding of novel ethanol-tolerant yeast strains.

Authors+Show Affiliations

Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjojima, Matsuoka-cho, Fukui 910-1195, Japan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17387467

Citation

Du, Xiaoyi, and Hiroshi Takagi. "N-Acetyltransferase Mpr1 Confers Ethanol Tolerance On Saccharomyces Cerevisiae By Reducing Reactive Oxygen Species." Applied Microbiology and Biotechnology, vol. 75, no. 6, 2007, pp. 1343-51.
Du X, Takagi H. N-Acetyltransferase Mpr1 confers ethanol tolerance on Saccharomyces cerevisiae by reducing reactive oxygen species. Appl Microbiol Biotechnol. 2007;75(6):1343-51.
Du, X., & Takagi, H. (2007). N-Acetyltransferase Mpr1 confers ethanol tolerance on Saccharomyces cerevisiae by reducing reactive oxygen species. Applied Microbiology and Biotechnology, 75(6), 1343-51.
Du X, Takagi H. N-Acetyltransferase Mpr1 Confers Ethanol Tolerance On Saccharomyces Cerevisiae By Reducing Reactive Oxygen Species. Appl Microbiol Biotechnol. 2007;75(6):1343-51. PubMed PMID: 17387467.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - N-Acetyltransferase Mpr1 confers ethanol tolerance on Saccharomyces cerevisiae by reducing reactive oxygen species. AU - Du,Xiaoyi, AU - Takagi,Hiroshi, Y1 - 2007/03/27/ PY - 2007/02/07/received PY - 2007/03/08/accepted PY - 2007/03/07/revised PY - 2007/3/28/pubmed PY - 2007/9/19/medline PY - 2007/3/28/entrez SP - 1343 EP - 51 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 75 IS - 6 N2 - N-Acetyltransferase Mpr1 of Saccharomyces cerevisiae can reduce intracellular oxidation levels and protect yeast cells under oxidative stress, including H(2)O(2), heat-shock, or freeze-thaw treatment. Unlike many antioxidant enzyme genes induced in response to oxidative stress, the MPR1 gene seems to be constitutively expressed in yeast cells. Based on a recent report that ethanol toxicity is correlated with the production of reactive oxygen species (ROS), we examined here the role of Mpr1 under ethanol stress conditions. The null mutant of the MPR1 and MPR2 genes showed hypersensitivity to ethanol stress, and the expression of the MPR1 gene conferred stress tolerance. We also found that yeast cells exhibited increased ROS levels during exposure to ethanol stress, and that Mpr1 protects yeast cells from ethanol stress by reducing intracellular ROS levels. When the MPR1 gene was overexpressed in antioxidant enzyme-deficient mutants, increased resistance to H(2)O(2) or heat shock was observed in cells lacking the CTA1, CTT1, or GPX1 gene encoding catalase A, catalase T, or glutathione peroxidase, respectively. These results suggest that Mpr1 might compensate the function of enzymes that detoxify H(2)O(2). Hence, Mpr1 has promising potential for the breeding of novel ethanol-tolerant yeast strains. SN - 0175-7598 UR - https://www.unboundmedicine.com/medline/citation/17387467/N_Acetyltransferase_Mpr1_confers_ethanol_tolerance_on_Saccharomyces_cerevisiae_by_reducing_reactive_oxygen_species_ L2 - https://dx.doi.org/10.1007/s00253-007-0940-x DB - PRIME DP - Unbound Medicine ER -