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Physical and biological properties of collagen-phospholipid polymer hybrid gels.

Abstract

We successfully developed a novel method for immobilizing poly(2-methacryloyloxyethyl phosphorylcholine) [Poly(MPC)] polymer onto collagen using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS) as cross-linkers. In order to obtain the highest possible molar ratio of immobilized MPC moieties on the collagen gel, a collagen-phospholipid polymer hybrid gel was prepared by repeating the cross-linking process up to three times to create a dense network of collagen and PMA. Network formation by repeating the immobilization process was successful, resulting in decreased free amine group content and a low swelling ratio. The hybrid gel displayed very high stability against degradation by collagenase and possessed high hydrophilicity. Fibrinogen adsorption and cell adhesion were reduced and demonstrated less cell proliferation as compared to that by uncross-linked collagen gel. The collagen-phospholipid polymer hybrid gel did not exhibit toxicity, and the cell morphology remained intact (round); this implies that the interaction between the cell and the collagen-phospholipid polymer hybrid gel is safe and mild.

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  • Authors+Show Affiliations

    ,

    Division of Biofunctional Molecules, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.

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    Source

    Biomaterials 28:20 2007 Jul pg 3153-62

    MeSH

    Absorption
    Animals
    Biocompatible Materials
    Cell Adhesion
    Cell Survival
    Collagen
    Collagenases
    Cross-Linking Reagents
    Ethyldimethylaminopropyl Carbodiimide
    Fibrinogen
    Fibroblasts
    Hydrogels
    Hydrogen-Ion Concentration
    L Cells (Cell Line)
    Mice
    Microscopy, Electron, Scanning
    Phospholipids
    Phosphorylcholine
    Polymers

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    17391753