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Identification and characterization of dominant helper T-cell epitopes in the nucleocapsid protein of severe acute respiratory syndrome coronavirus.
J Virol. 2007 Jun; 81(11):6079-88.JV

Abstract

By using a series of overlapping synthetic peptides covering 98% of the amino acid sequence of the nucleocapsid protein (NP) of severe acute respiratory syndrome coronavirus (SARS-CoV), four helper T-cell (Th) epitopes (NP11, residues 11 to 25; NP51, residues 51 to 65; NP61, residues 61 to 75; and NP111, residues 111 to 125) in C57BL mice (H-2(b)), four (NP21, residues 21 to 35; NP91, residues 91 to 105; NP331, residues 331 to 345; and NP351, residues 351 to 365) in C3H mice (H-2(k)), and two (NP81, residues 81 to 95; and NP351, residues 351 to 365) in BALB/c mice (H-2(d)) have been identified. All of these peptides were able to stimulate the proliferation of NP-specific T-cell lines or freshly isolated lymph node cells from mice immunized with recombinant NP. Immunization of mice with synthetic peptides containing appropriate Th epitopes elicited strong cellular immunity in vivo, as evidenced by delayed-type hypersensitivity. Priming with the helper peptides (e.g., NP111 and NP351) significantly accelerated the immune response induced by recombinant NP, as determined by the production of NP-specific antibodies. When fused with a conserved neutralizing epitope (SP1143-1157) from the spike protein of SARS-CoV, NP111 and NP351 assisted in the production of high-titer neutralizing antibodies in vivo. These data provide useful insights regarding immunity against SARS-CoV and have the potential to help guide the design of peptide-based vaccines.

Authors+Show Affiliations

Department of Immunology, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100083, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17392374

Citation

Zhao, Jincun, et al. "Identification and Characterization of Dominant Helper T-cell Epitopes in the Nucleocapsid Protein of Severe Acute Respiratory Syndrome Coronavirus." Journal of Virology, vol. 81, no. 11, 2007, pp. 6079-88.
Zhao J, Huang Q, Wang W, et al. Identification and characterization of dominant helper T-cell epitopes in the nucleocapsid protein of severe acute respiratory syndrome coronavirus. J Virol. 2007;81(11):6079-88.
Zhao, J., Huang, Q., Wang, W., Zhang, Y., Lv, P., & Gao, X. M. (2007). Identification and characterization of dominant helper T-cell epitopes in the nucleocapsid protein of severe acute respiratory syndrome coronavirus. Journal of Virology, 81(11), 6079-88.
Zhao J, et al. Identification and Characterization of Dominant Helper T-cell Epitopes in the Nucleocapsid Protein of Severe Acute Respiratory Syndrome Coronavirus. J Virol. 2007;81(11):6079-88. PubMed PMID: 17392374.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of dominant helper T-cell epitopes in the nucleocapsid protein of severe acute respiratory syndrome coronavirus. AU - Zhao,Jincun, AU - Huang,Qianrong, AU - Wang,Wei, AU - Zhang,Yan, AU - Lv,Ping, AU - Gao,Xiao-Ming, Y1 - 2007/03/28/ PY - 2007/3/30/pubmed PY - 2007/7/13/medline PY - 2007/3/30/entrez SP - 6079 EP - 88 JF - Journal of virology JO - J Virol VL - 81 IS - 11 N2 - By using a series of overlapping synthetic peptides covering 98% of the amino acid sequence of the nucleocapsid protein (NP) of severe acute respiratory syndrome coronavirus (SARS-CoV), four helper T-cell (Th) epitopes (NP11, residues 11 to 25; NP51, residues 51 to 65; NP61, residues 61 to 75; and NP111, residues 111 to 125) in C57BL mice (H-2(b)), four (NP21, residues 21 to 35; NP91, residues 91 to 105; NP331, residues 331 to 345; and NP351, residues 351 to 365) in C3H mice (H-2(k)), and two (NP81, residues 81 to 95; and NP351, residues 351 to 365) in BALB/c mice (H-2(d)) have been identified. All of these peptides were able to stimulate the proliferation of NP-specific T-cell lines or freshly isolated lymph node cells from mice immunized with recombinant NP. Immunization of mice with synthetic peptides containing appropriate Th epitopes elicited strong cellular immunity in vivo, as evidenced by delayed-type hypersensitivity. Priming with the helper peptides (e.g., NP111 and NP351) significantly accelerated the immune response induced by recombinant NP, as determined by the production of NP-specific antibodies. When fused with a conserved neutralizing epitope (SP1143-1157) from the spike protein of SARS-CoV, NP111 and NP351 assisted in the production of high-titer neutralizing antibodies in vivo. These data provide useful insights regarding immunity against SARS-CoV and have the potential to help guide the design of peptide-based vaccines. SN - 0022-538X UR - https://www.unboundmedicine.com/medline/citation/17392374/Identification_and_characterization_of_dominant_helper_T_cell_epitopes_in_the_nucleocapsid_protein_of_severe_acute_respiratory_syndrome_coronavirus_ L2 - http://jvi.asm.org/cgi/pmidlookup?view=long&pmid=17392374 DB - PRIME DP - Unbound Medicine ER -