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Human single-chain Fv antibodies against Burkholderia mallei and Burkholderia pseudomallei.
Exp Biol Med (Maywood). 2007 Apr; 232(4):550-6.EB

Abstract

Much effort has been devoted to the development of mouse monoclonal antibodies that react specifically with Burkholderia mallei and Burkholderia pseudomallei for diagnostic and/or therapeutic purposes. Our present study focused on the screening of a phage-displayed nonimmune human single-chain Fv (scFv) antibody library against heat-killed B. mallei and B. pseudomallei for the generation of human scFv antibodies specific to the two pathogenic species of bacteria. Using two different panning procedures, we obtained seven different scFv phage antibodies that interacted with the heat-killed whole bacterial cells of B. mallei and B. pseudomallei. Our results demonstrate that panning of a human scFv antibody library against heat-killed whole bacterial cells may provide a valuable strategy for developing human monoclonal antibodies against the highly pathogenic bacteria.

Authors+Show Affiliations

Department of Environmental and Infectious Diseases, American Registry of Pathology, Armed Forces Institute of Pathology, Washington, DC 20306, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17392491

Citation

Zou, Nianxiang, et al. "Human Single-chain Fv Antibodies Against Burkholderia Mallei and Burkholderia Pseudomallei." Experimental Biology and Medicine (Maywood, N.J.), vol. 232, no. 4, 2007, pp. 550-6.
Zou N, Newsome T, Li B, et al. Human single-chain Fv antibodies against Burkholderia mallei and Burkholderia pseudomallei. Exp Biol Med (Maywood). 2007;232(4):550-6.
Zou, N., Newsome, T., Li, B., Tsai, S., & Lo, S. C. (2007). Human single-chain Fv antibodies against Burkholderia mallei and Burkholderia pseudomallei. Experimental Biology and Medicine (Maywood, N.J.), 232(4), 550-6.
Zou N, et al. Human Single-chain Fv Antibodies Against Burkholderia Mallei and Burkholderia Pseudomallei. Exp Biol Med (Maywood). 2007;232(4):550-6. PubMed PMID: 17392491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human single-chain Fv antibodies against Burkholderia mallei and Burkholderia pseudomallei. AU - Zou,Nianxiang, AU - Newsome,Tamara, AU - Li,Bingjie, AU - Tsai,Shien, AU - Lo,Shyh-Ching, PY - 2007/3/30/pubmed PY - 2007/5/10/medline PY - 2007/3/30/entrez SP - 550 EP - 6 JF - Experimental biology and medicine (Maywood, N.J.) JO - Exp Biol Med (Maywood) VL - 232 IS - 4 N2 - Much effort has been devoted to the development of mouse monoclonal antibodies that react specifically with Burkholderia mallei and Burkholderia pseudomallei for diagnostic and/or therapeutic purposes. Our present study focused on the screening of a phage-displayed nonimmune human single-chain Fv (scFv) antibody library against heat-killed B. mallei and B. pseudomallei for the generation of human scFv antibodies specific to the two pathogenic species of bacteria. Using two different panning procedures, we obtained seven different scFv phage antibodies that interacted with the heat-killed whole bacterial cells of B. mallei and B. pseudomallei. Our results demonstrate that panning of a human scFv antibody library against heat-killed whole bacterial cells may provide a valuable strategy for developing human monoclonal antibodies against the highly pathogenic bacteria. SN - 1535-3702 UR - https://www.unboundmedicine.com/medline/citation/17392491/Human_single_chain_Fv_antibodies_against_Burkholderia_mallei_and_Burkholderia_pseudomallei_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=17392491.ui DB - PRIME DP - Unbound Medicine ER -