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Loss of CO from the intravascular bed and its impact on the optimised CO-rebreathing method.
Eur J Appl Physiol. 2007 Jul; 100(4):383-91.EJ

Abstract

Total haemoglobin mass can be easily measured by applying the optimised CO-rebreathing method (oCOR-method). Prerequisite for its accurate determination is a homogenous CO distribution in the blood and the exact knowledge of the CO volume circulating in the vascular space. The aim of the study was to evaluate the mixing time of CO in the blood after inhaling a CO-bolus and to quantify the CO volume leaving the vascular bed due to diffusion to myoglobin and due to exhalation during processing the oCOR-method. The oCOR-method was also compared to a former commonly used CO-rebreathing procedure. In ten subjects, the time course of carboxy-haemoglobin (HbCO) formation was analysed simultaneously in capillary and venous blood for a period of 15 min after inhaling a CO bolus. The volume of CO diffusing from haemoglobin to myoglobin was calculated via the decrease of HbCO. As part of this decrease is due to CO exhalation, this volume was quantified by collecting the exhaled air in a Douglas bag system. Equal HbCO values in capillary and venous blood were reached at min 6 indicating complete mixing of CO. The loss of CO out of the vascular bed due to exhalation and due to diffusion to myoglobin was 0.32 +/- 0.12% min(-1) (0.25 +/- 0.09 ml min(-1)) and 0.32 +/- 0.18% min(-1) (0.24 +/- 0.13 ml min(-1)) of the administered CO volume, respectively. The loss of CO due to exhalation and diffusion to myoglobin is of minor impact. It should, however, be considered by using correction factors to obtain high accuracy when determining total haemoglobin mass.

Authors+Show Affiliations

Prommer N
Department of Sports Medicine and Sports Physiology, University of Bayreuth, Universitaetsstrasse 30, 95447 Bayreuth, Germany.
Schmidt W
No affiliation info available

MeSH

Administration, InhalationAdultCarbon MonoxideCarboxyhemoglobinDiffusionHemoglobinsHumansMaleMyoglobinPulmonary Diffusing CapacityPulmonary Gas ExchangeReproducibility of Results

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17394011

Citation

Prommer, Nicole, and Walter Schmidt. "Loss of CO From the Intravascular Bed and Its Impact On the Optimised CO-rebreathing Method." European Journal of Applied Physiology, vol. 100, no. 4, 2007, pp. 383-91.
Prommer N, Schmidt W. Loss of CO from the intravascular bed and its impact on the optimised CO-rebreathing method. Eur J Appl Physiol. 2007;100(4):383-91.
Prommer, N., & Schmidt, W. (2007). Loss of CO from the intravascular bed and its impact on the optimised CO-rebreathing method. European Journal of Applied Physiology, 100(4), 383-91.
Prommer N, Schmidt W. Loss of CO From the Intravascular Bed and Its Impact On the Optimised CO-rebreathing Method. Eur J Appl Physiol. 2007;100(4):383-91. PubMed PMID: 17394011.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Loss of CO from the intravascular bed and its impact on the optimised CO-rebreathing method. AU - Prommer,Nicole, AU - Schmidt,Walter, Y1 - 2007/03/30/ PY - 2007/03/05/accepted PY - 2007/3/31/pubmed PY - 2007/10/20/medline PY - 2007/3/31/entrez SP - 383 EP - 91 JF - European journal of applied physiology JO - Eur J Appl Physiol VL - 100 IS - 4 N2 - Total haemoglobin mass can be easily measured by applying the optimised CO-rebreathing method (oCOR-method). Prerequisite for its accurate determination is a homogenous CO distribution in the blood and the exact knowledge of the CO volume circulating in the vascular space. The aim of the study was to evaluate the mixing time of CO in the blood after inhaling a CO-bolus and to quantify the CO volume leaving the vascular bed due to diffusion to myoglobin and due to exhalation during processing the oCOR-method. The oCOR-method was also compared to a former commonly used CO-rebreathing procedure. In ten subjects, the time course of carboxy-haemoglobin (HbCO) formation was analysed simultaneously in capillary and venous blood for a period of 15 min after inhaling a CO bolus. The volume of CO diffusing from haemoglobin to myoglobin was calculated via the decrease of HbCO. As part of this decrease is due to CO exhalation, this volume was quantified by collecting the exhaled air in a Douglas bag system. Equal HbCO values in capillary and venous blood were reached at min 6 indicating complete mixing of CO. The loss of CO out of the vascular bed due to exhalation and due to diffusion to myoglobin was 0.32 +/- 0.12% min(-1) (0.25 +/- 0.09 ml min(-1)) and 0.32 +/- 0.18% min(-1) (0.24 +/- 0.13 ml min(-1)) of the administered CO volume, respectively. The loss of CO due to exhalation and diffusion to myoglobin is of minor impact. It should, however, be considered by using correction factors to obtain high accuracy when determining total haemoglobin mass. SN - 1439-6319 UR - https://www.unboundmedicine.com/medline/citation/17394011/Loss_of_CO_from_the_intravascular_bed_and_its_impact_on_the_optimised_CO_rebreathing_method_ DB - PRIME DP - Unbound Medicine ER -