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Interaction of lipophilic moisturizers on stratum corneum lipid domains in vitro and in vivo.
Skin Pharmacol Physiol. 2007; 20(4):175-86.SP

Abstract

Dry skin symptoms such as scaling and itching are often treated with lipophilic moisturizers. The aim of this study was to investigate the effect of lipophilic moisturizers on the stratum corneum (SC) ultra-structure and lipid organization. Lipophilic moisturizers were applied on the forearms of 4 healthy volunteers for 3 h. Subsequently, the application sites were tape stripped, and selected tape strips prepared for Freeze Fracture Electron Microscopy (FFEM), a method to visualize the SC intercellular lipid parallel to the skin surface. To investigate the effect of lipid moisturizers on the lipid lamellae, isolated SC was pretreated with the lipophilic moisturizers for 24 h prior to performing small angle X-ray diffraction (SAXD) measurements. Additionally, the lipid organization of mixtures prepared with ceramides, cholesterol, free fatty acids and lipophilic moisturizer in a 2:1:1:1 molar ratio were studied using SAXD. The FFEM data (in vivo) as well as the SAXD data (in vitro) show that the lipophilic moisturizers do not change the lipid lamellar organization in the SC. Addition of 20% m/m lipophilic moisturizer to the ceramide:cholesterol:free fatty acids mixture did not inhibit the formation of the long periodicity phase, the characteristic lamellar phase in the SC, even though there was clear evidence that two of the three moisturizers were at least partially incorporated in the long periodicity phase. Concluding, all findings suggest that the lipophilic moisturizers investigated in this study do not drastically change the lamellar organization of the SC intracellular lipid matrix, but that the moisturizers form separate domains in the SC, as was visualized by FFEM.

Authors+Show Affiliations

Department of Drug Delivery Technology, Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17396052

Citation

Caussin, J, et al. "Interaction of Lipophilic Moisturizers On Stratum Corneum Lipid Domains in Vitro and in Vivo." Skin Pharmacology and Physiology, vol. 20, no. 4, 2007, pp. 175-86.
Caussin J, Gooris GS, Groenink HW, et al. Interaction of lipophilic moisturizers on stratum corneum lipid domains in vitro and in vivo. Skin Pharmacol Physiol. 2007;20(4):175-86.
Caussin, J., Gooris, G. S., Groenink, H. W., Wiechers, J. W., & Bouwstra, J. A. (2007). Interaction of lipophilic moisturizers on stratum corneum lipid domains in vitro and in vivo. Skin Pharmacology and Physiology, 20(4), 175-86.
Caussin J, et al. Interaction of Lipophilic Moisturizers On Stratum Corneum Lipid Domains in Vitro and in Vivo. Skin Pharmacol Physiol. 2007;20(4):175-86. PubMed PMID: 17396052.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interaction of lipophilic moisturizers on stratum corneum lipid domains in vitro and in vivo. AU - Caussin,J, AU - Gooris,G S, AU - Groenink,H W W, AU - Wiechers,J W, AU - Bouwstra,J A, Y1 - 2007/03/29/ PY - 2006/07/13/received PY - 2006/11/23/accepted PY - 2007/3/31/pubmed PY - 2007/8/19/medline PY - 2007/3/31/entrez SP - 175 EP - 86 JF - Skin pharmacology and physiology JO - Skin Pharmacol Physiol VL - 20 IS - 4 N2 - Dry skin symptoms such as scaling and itching are often treated with lipophilic moisturizers. The aim of this study was to investigate the effect of lipophilic moisturizers on the stratum corneum (SC) ultra-structure and lipid organization. Lipophilic moisturizers were applied on the forearms of 4 healthy volunteers for 3 h. Subsequently, the application sites were tape stripped, and selected tape strips prepared for Freeze Fracture Electron Microscopy (FFEM), a method to visualize the SC intercellular lipid parallel to the skin surface. To investigate the effect of lipid moisturizers on the lipid lamellae, isolated SC was pretreated with the lipophilic moisturizers for 24 h prior to performing small angle X-ray diffraction (SAXD) measurements. Additionally, the lipid organization of mixtures prepared with ceramides, cholesterol, free fatty acids and lipophilic moisturizer in a 2:1:1:1 molar ratio were studied using SAXD. The FFEM data (in vivo) as well as the SAXD data (in vitro) show that the lipophilic moisturizers do not change the lipid lamellar organization in the SC. Addition of 20% m/m lipophilic moisturizer to the ceramide:cholesterol:free fatty acids mixture did not inhibit the formation of the long periodicity phase, the characteristic lamellar phase in the SC, even though there was clear evidence that two of the three moisturizers were at least partially incorporated in the long periodicity phase. Concluding, all findings suggest that the lipophilic moisturizers investigated in this study do not drastically change the lamellar organization of the SC intracellular lipid matrix, but that the moisturizers form separate domains in the SC, as was visualized by FFEM. SN - 1660-5527 UR - https://www.unboundmedicine.com/medline/citation/17396052/Interaction_of_lipophilic_moisturizers_on_stratum_corneum_lipid_domains_in_vitro_and_in_vivo_ L2 - https://www.karger.com?DOI=10.1159/000101387 DB - PRIME DP - Unbound Medicine ER -