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Mucin expression profile in Barrett's, dysplasia, adenocarcinoma sequence in the esophagus.
Indian J Cancer. 2007 Jan-Mar; 44(1):1-5.IJ

Abstract

BACKGROUND

The molecular events that accompany the progression to adenocarcinoma (ADC) of the esophagus are poorly understood. Aberrant mucin receptor expression can contribute to increased cell growth and metastatic ability.

AIM

The aim of this study was to establish a pattern for mucin (MUC) gene expression in the esophageal mucosa under normal and pathological conditions.

SETTING

University Hospital Cancer Center Laboratory. Archived tissue samples studied in a retrospective fashion.

MATERIALS AND METHODS

Tissue samples were obtained from the archives of patients with histological evidence of Barrett's esophagus (BE) progressing to ADC. Immunohistochemical analysis was performed using mouse monoclonal antibodies for MUC1, MUC2, MUC5AC, MUC6. Semiquantitative scoring of histological staining was performed using a linear scoring system: 0-staining absent; 1-staining in 0-25%; 2-staining in 25-50%; and 3-staining in 50-75% of the epithelium. The Binomial test was used to explore trends and differences in frequency of mucin expression along the pathological sequence.

RESULTS

Only mild superficial staining of MUC1 was seen in normal squamous epithelium. MUC1 and MUC2 were uniformly expressed in all samples (7/7) of BE and dysplasia (P=0.008). MUC1 expression was upregulated (7/7) in progression to adenocarcinoma (P=0.008). The secretory mucins, MUC5AC and MUC6 showed a decrease in expression with progression from BE to dysplasia to ADC (P< 0.05).

CONCLUSIONS

Downregulation of MUC5AC and MUC6 decreases mucosal protection against gastric acid. Increasing MUC1 expression is associated with progression from dysplasia to ADC. Upregulation of MUC2 reflects intestinal metaplasia in BE.

Authors+Show Affiliations

Department of Surgical Oncology, Creighton University, Omaha, NE 68131, USA. sathyabc@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17401217

Citation

Burjonrappa, S C., et al. "Mucin Expression Profile in Barrett's, Dysplasia, Adenocarcinoma Sequence in the Esophagus." Indian Journal of Cancer, vol. 44, no. 1, 2007, pp. 1-5.
Burjonrappa SC, Reddimasu S, Nawaz Z, et al. Mucin expression profile in Barrett's, dysplasia, adenocarcinoma sequence in the esophagus. Indian J Cancer. 2007;44(1):1-5.
Burjonrappa, S. C., Reddimasu, S., Nawaz, Z., Gao, X., Sharma, P., & Loggie, B. (2007). Mucin expression profile in Barrett's, dysplasia, adenocarcinoma sequence in the esophagus. Indian Journal of Cancer, 44(1), 1-5.
Burjonrappa SC, et al. Mucin Expression Profile in Barrett's, Dysplasia, Adenocarcinoma Sequence in the Esophagus. Indian J Cancer. 2007 Jan-Mar;44(1):1-5. PubMed PMID: 17401217.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mucin expression profile in Barrett's, dysplasia, adenocarcinoma sequence in the esophagus. AU - Burjonrappa,S C, AU - Reddimasu,S, AU - Nawaz,Z, AU - Gao,X, AU - Sharma,P, AU - Loggie,B, PY - 2007/4/3/pubmed PY - 2007/5/30/medline PY - 2007/4/3/entrez SP - 1 EP - 5 JF - Indian journal of cancer JO - Indian J Cancer VL - 44 IS - 1 N2 - BACKGROUND: The molecular events that accompany the progression to adenocarcinoma (ADC) of the esophagus are poorly understood. Aberrant mucin receptor expression can contribute to increased cell growth and metastatic ability. AIM: The aim of this study was to establish a pattern for mucin (MUC) gene expression in the esophageal mucosa under normal and pathological conditions. SETTING: University Hospital Cancer Center Laboratory. Archived tissue samples studied in a retrospective fashion. MATERIALS AND METHODS: Tissue samples were obtained from the archives of patients with histological evidence of Barrett's esophagus (BE) progressing to ADC. Immunohistochemical analysis was performed using mouse monoclonal antibodies for MUC1, MUC2, MUC5AC, MUC6. Semiquantitative scoring of histological staining was performed using a linear scoring system: 0-staining absent; 1-staining in 0-25%; 2-staining in 25-50%; and 3-staining in 50-75% of the epithelium. The Binomial test was used to explore trends and differences in frequency of mucin expression along the pathological sequence. RESULTS: Only mild superficial staining of MUC1 was seen in normal squamous epithelium. MUC1 and MUC2 were uniformly expressed in all samples (7/7) of BE and dysplasia (P=0.008). MUC1 expression was upregulated (7/7) in progression to adenocarcinoma (P=0.008). The secretory mucins, MUC5AC and MUC6 showed a decrease in expression with progression from BE to dysplasia to ADC (P< 0.05). CONCLUSIONS: Downregulation of MUC5AC and MUC6 decreases mucosal protection against gastric acid. Increasing MUC1 expression is associated with progression from dysplasia to ADC. Upregulation of MUC2 reflects intestinal metaplasia in BE. SN - 0019-509X UR - https://www.unboundmedicine.com/medline/citation/17401217/Mucin_expression_profile_in_Barrett's_dysplasia_adenocarcinoma_sequence_in_the_esophagus_ L2 - http://www.indianjcancer.com/article.asp?issn=0019-509X;year=2007;volume=44;issue=1;spage=1;epage=5;aulast=Burjonrappa DB - PRIME DP - Unbound Medicine ER -