Tags

Type your tag names separated by a space and hit enter

Depletion of the yeast nuclear exosome subunit Rrp6 results in accumulation of polyadenylated RNAs in a discrete domain within the nucleolus.
Mol Cell Biol. 2007 Jun; 27(11):4157-65.MC

Abstract

Recent data reveal that a substantial fraction of transcripts generated by RNA polymerases I, II, and III are rapidly degraded in the nucleus by the combined action of the exosome and a noncanonical poly(A) polymerase activity. This work identifies a domain within the yeast nucleolus that is enriched in polyadenylated RNAs in the absence of the nuclear exosome RNase Rrp6 or the exosome cofactor Mtr4. In normal yeast cells, poly(A)(+) RNA was undetectable in the nucleolus but the depletion of either Rrp6 or Mtr4 led to the accumulation of polyadenylated RNAs in a discrete subnucleolar region. This nucleolar poly(A) domain is enriched for the U14 snoRNA and the snoRNP protein Nop1 but is distinct from the nucleolar body that functions in snoRNA maturation. In strains lacking both Rrp6 and the poly(A) polymerase Trf4, the accumulation of poly(A)(+) RNA was suppressed, suggesting the involvement of the Trf4-Air1/2-Mtr4 polyadenylation (TRAMP) complex. The accumulation of polyadenylated snoRNAs in a discrete nucleolar domain may promote their recognition as substrates for the exosome.

Authors+Show Affiliations

Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisbon, Portugal.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17403903

Citation

Carneiro, Tiago, et al. "Depletion of the Yeast Nuclear Exosome Subunit Rrp6 Results in Accumulation of Polyadenylated RNAs in a Discrete Domain Within the Nucleolus." Molecular and Cellular Biology, vol. 27, no. 11, 2007, pp. 4157-65.
Carneiro T, Carvalho C, Braga J, et al. Depletion of the yeast nuclear exosome subunit Rrp6 results in accumulation of polyadenylated RNAs in a discrete domain within the nucleolus. Mol Cell Biol. 2007;27(11):4157-65.
Carneiro, T., Carvalho, C., Braga, J., Rino, J., Milligan, L., Tollervey, D., & Carmo-Fonseca, M. (2007). Depletion of the yeast nuclear exosome subunit Rrp6 results in accumulation of polyadenylated RNAs in a discrete domain within the nucleolus. Molecular and Cellular Biology, 27(11), 4157-65.
Carneiro T, et al. Depletion of the Yeast Nuclear Exosome Subunit Rrp6 Results in Accumulation of Polyadenylated RNAs in a Discrete Domain Within the Nucleolus. Mol Cell Biol. 2007;27(11):4157-65. PubMed PMID: 17403903.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Depletion of the yeast nuclear exosome subunit Rrp6 results in accumulation of polyadenylated RNAs in a discrete domain within the nucleolus. AU - Carneiro,Tiago, AU - Carvalho,Célia, AU - Braga,José, AU - Rino,José, AU - Milligan,Laura, AU - Tollervey,David, AU - Carmo-Fonseca,Maria, Y1 - 2007/04/02/ PY - 2007/4/4/pubmed PY - 2007/7/21/medline PY - 2007/4/4/entrez SP - 4157 EP - 65 JF - Molecular and cellular biology JO - Mol Cell Biol VL - 27 IS - 11 N2 - Recent data reveal that a substantial fraction of transcripts generated by RNA polymerases I, II, and III are rapidly degraded in the nucleus by the combined action of the exosome and a noncanonical poly(A) polymerase activity. This work identifies a domain within the yeast nucleolus that is enriched in polyadenylated RNAs in the absence of the nuclear exosome RNase Rrp6 or the exosome cofactor Mtr4. In normal yeast cells, poly(A)(+) RNA was undetectable in the nucleolus but the depletion of either Rrp6 or Mtr4 led to the accumulation of polyadenylated RNAs in a discrete subnucleolar region. This nucleolar poly(A) domain is enriched for the U14 snoRNA and the snoRNP protein Nop1 but is distinct from the nucleolar body that functions in snoRNA maturation. In strains lacking both Rrp6 and the poly(A) polymerase Trf4, the accumulation of poly(A)(+) RNA was suppressed, suggesting the involvement of the Trf4-Air1/2-Mtr4 polyadenylation (TRAMP) complex. The accumulation of polyadenylated snoRNAs in a discrete nucleolar domain may promote their recognition as substrates for the exosome. SN - 0270-7306 UR - https://www.unboundmedicine.com/medline/citation/17403903/Depletion_of_the_yeast_nuclear_exosome_subunit_Rrp6_results_in_accumulation_of_polyadenylated_RNAs_in_a_discrete_domain_within_the_nucleolus_ DB - PRIME DP - Unbound Medicine ER -