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Plasma levels and adipose tissue messenger ribonucleic acid expression of retinol-binding protein 4 are reduced during calorie restriction in obese subjects but are not related to diet-induced changes in insulin sensitivity.
J Clin Endocrinol Metab. 2007 Jun; 92(6):2330-5.JC

Abstract

CONTEXT

Retinol-binding protein 4 (RBP4) may play a role in the development of insulin resistance.

OBJECTIVE

We investigated whether RBP4 adipose tissue mRNA expression and plasma level are related to insulin sensitivity during a diet-induced weight loss.

DESIGN, SETTING, PATIENTS, AND INTERVENTION

Obese women followed a dietary intervention composed of a 4-wk very low-calorie diet (VLCD), a 2-month low-calorie diet, and 3-4 months of a weight maintenance (WM) phase.

MAIN OUTCOME MEASURES

Clinical investigation was performed before and at the end of each phase. Insulin sensitivity was assessed with the euglycemic hyperinsulinemic clamp. Adipose tissue mRNA and plasma levels of RBP4 were determined using reverse transcription-quantitative PCR and ELISA, respectively.

RESULTS

Weight and fat mass decreased during VLCD and were stabilized during WM. Glucose disposal rate increased during VLCD and remained elevated thereafter. Plasma levels of RBP4 decreased after VLCD and, although increasing at subsequent phases, remained lower than prediet values. Adipose tissue mRNA levels were diminished after VLCD, and increased during low-calorie diet and WM to reach basal values. Basal RBP4 levels or diet-induced variations of RBP4 were not different in lean women and two groups of obese women with high- and low-insulin sensitivity.

CONCLUSIONS

Severe calorie restriction promotes a reduction in adipose tissue and plasma levels of RBP4. The study does not bring evidence for a role for RBP4 in the regulation of diet-induced changes in insulin sensitivity.

Authors+Show Affiliations

Institut National de la Santé et de la Recherche Médicale Université Paul Sabatier U858, IFR31, BP 84225, 31432 Toulouse Cedex 4, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17405846

Citation

Vitkova, Michaela, et al. "Plasma Levels and Adipose Tissue Messenger Ribonucleic Acid Expression of Retinol-binding Protein 4 Are Reduced During Calorie Restriction in Obese Subjects but Are Not Related to Diet-induced Changes in Insulin Sensitivity." The Journal of Clinical Endocrinology and Metabolism, vol. 92, no. 6, 2007, pp. 2330-5.
Vitkova M, Klimcakova E, Kovacikova M, et al. Plasma levels and adipose tissue messenger ribonucleic acid expression of retinol-binding protein 4 are reduced during calorie restriction in obese subjects but are not related to diet-induced changes in insulin sensitivity. J Clin Endocrinol Metab. 2007;92(6):2330-5.
Vitkova, M., Klimcakova, E., Kovacikova, M., Valle, C., Moro, C., Polak, J., Hanacek, J., Capel, F., Viguerie, N., Richterova, B., Bajzova, M., Hejnova, J., Stich, V., & Langin, D. (2007). Plasma levels and adipose tissue messenger ribonucleic acid expression of retinol-binding protein 4 are reduced during calorie restriction in obese subjects but are not related to diet-induced changes in insulin sensitivity. The Journal of Clinical Endocrinology and Metabolism, 92(6), 2330-5.
Vitkova M, et al. Plasma Levels and Adipose Tissue Messenger Ribonucleic Acid Expression of Retinol-binding Protein 4 Are Reduced During Calorie Restriction in Obese Subjects but Are Not Related to Diet-induced Changes in Insulin Sensitivity. J Clin Endocrinol Metab. 2007;92(6):2330-5. PubMed PMID: 17405846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plasma levels and adipose tissue messenger ribonucleic acid expression of retinol-binding protein 4 are reduced during calorie restriction in obese subjects but are not related to diet-induced changes in insulin sensitivity. AU - Vitkova,Michaela, AU - Klimcakova,Eva, AU - Kovacikova,Michaela, AU - Valle,Carine, AU - Moro,Cédric, AU - Polak,Jan, AU - Hanacek,Jiri, AU - Capel,Frédéric, AU - Viguerie,Nathalie, AU - Richterova,Blanka, AU - Bajzova,Magda, AU - Hejnova,Jindra, AU - Stich,Vladimir, AU - Langin,Dominique, Y1 - 2007/04/03/ PY - 2007/4/5/pubmed PY - 2007/7/19/medline PY - 2007/4/5/entrez SP - 2330 EP - 5 JF - The Journal of clinical endocrinology and metabolism JO - J Clin Endocrinol Metab VL - 92 IS - 6 N2 - CONTEXT: Retinol-binding protein 4 (RBP4) may play a role in the development of insulin resistance. OBJECTIVE: We investigated whether RBP4 adipose tissue mRNA expression and plasma level are related to insulin sensitivity during a diet-induced weight loss. DESIGN, SETTING, PATIENTS, AND INTERVENTION: Obese women followed a dietary intervention composed of a 4-wk very low-calorie diet (VLCD), a 2-month low-calorie diet, and 3-4 months of a weight maintenance (WM) phase. MAIN OUTCOME MEASURES: Clinical investigation was performed before and at the end of each phase. Insulin sensitivity was assessed with the euglycemic hyperinsulinemic clamp. Adipose tissue mRNA and plasma levels of RBP4 were determined using reverse transcription-quantitative PCR and ELISA, respectively. RESULTS: Weight and fat mass decreased during VLCD and were stabilized during WM. Glucose disposal rate increased during VLCD and remained elevated thereafter. Plasma levels of RBP4 decreased after VLCD and, although increasing at subsequent phases, remained lower than prediet values. Adipose tissue mRNA levels were diminished after VLCD, and increased during low-calorie diet and WM to reach basal values. Basal RBP4 levels or diet-induced variations of RBP4 were not different in lean women and two groups of obese women with high- and low-insulin sensitivity. CONCLUSIONS: Severe calorie restriction promotes a reduction in adipose tissue and plasma levels of RBP4. The study does not bring evidence for a role for RBP4 in the regulation of diet-induced changes in insulin sensitivity. SN - 0021-972X UR - https://www.unboundmedicine.com/medline/citation/17405846/Plasma_levels_and_adipose_tissue_messenger_ribonucleic_acid_expression_of_retinol_binding_protein_4_are_reduced_during_calorie_restriction_in_obese_subjects_but_are_not_related_to_diet_induced_changes_in_insulin_sensitivity_ L2 - https://academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2006-2668 DB - PRIME DP - Unbound Medicine ER -