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A simple method for quantitative determination of polysaccharides in fungal cell walls.
Nat Protoc. 2006; 1(6):2995-3000.NP

Abstract

A simple and reliable method for quantitative determination of cell wall polymers in fungal cell with an s.e.m. of 5% is described. This protocol is based on the hydrolysis by sulfuric acid of beta-glucan, mannan, galactomannan and chitin present at different levels in the wall of yeasts and filamentous fungi into their corresponding monomers glucose, mannose, galactose and glucosamine. The released monosaccharides are subsequently separated and quantified by high-performance ionic chromatography coupled to pulse amperometry detection, with a detection limit of 1.0 mug ml(-1). This procedure is well suited to screening a large collection of yeast mutants or to evaluating effects of environmental conditions on cell wall polysaccharide content. This procedure is also applicable to other fungal species, including Schizosaccharomyces pombe, Candida albicans and Aspergillus fumigatus. Results can be obtained in 3 d.

Authors+Show Affiliations

François JM
Laboratoire de Biotechnologie & Bioprocédés, UMR-CNRS 5504 and INRA 792, Institut National des Sciences Appliquées, 135 Avenue de Rangueil, 31077 Toulouse cedex 04, France.

MeSH

Cell WallChromatography, Ion ExchangeElectricityFungiPolysaccharides

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17406560

Citation

François, Jean Marie. "A Simple Method for Quantitative Determination of Polysaccharides in Fungal Cell Walls." Nature Protocols, vol. 1, no. 6, 2006, pp. 2995-3000.
François JM. A simple method for quantitative determination of polysaccharides in fungal cell walls. Nat Protoc. 2006;1(6):2995-3000.
François, J. M. (2006). A simple method for quantitative determination of polysaccharides in fungal cell walls. Nature Protocols, 1(6), 2995-3000.
François JM. A Simple Method for Quantitative Determination of Polysaccharides in Fungal Cell Walls. Nat Protoc. 2006;1(6):2995-3000. PubMed PMID: 17406560.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A simple method for quantitative determination of polysaccharides in fungal cell walls. A1 - François,Jean Marie, PY - 2007/4/5/pubmed PY - 2007/11/6/medline PY - 2007/4/5/entrez SP - 2995 EP - 3000 JF - Nature protocols JO - Nat Protoc VL - 1 IS - 6 N2 - A simple and reliable method for quantitative determination of cell wall polymers in fungal cell with an s.e.m. of 5% is described. This protocol is based on the hydrolysis by sulfuric acid of beta-glucan, mannan, galactomannan and chitin present at different levels in the wall of yeasts and filamentous fungi into their corresponding monomers glucose, mannose, galactose and glucosamine. The released monosaccharides are subsequently separated and quantified by high-performance ionic chromatography coupled to pulse amperometry detection, with a detection limit of 1.0 mug ml(-1). This procedure is well suited to screening a large collection of yeast mutants or to evaluating effects of environmental conditions on cell wall polysaccharide content. This procedure is also applicable to other fungal species, including Schizosaccharomyces pombe, Candida albicans and Aspergillus fumigatus. Results can be obtained in 3 d. SN - 1750-2799 UR - https://www.unboundmedicine.com/medline/citation/17406560/A_simple_method_for_quantitative_determination_of_polysaccharides_in_fungal_cell_walls_ L2 - https://doi.org/10.1038/nprot.2006.457 DB - PRIME DP - Unbound Medicine ER -