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Dissecting mechanisms of nuclear mRNA surveillance in THO/sub2 complex mutants.
EMBO J. 2007 May 02; 26(9):2317-26.EJ

Abstract

The nuclear exosome is involved in numerous RNA metabolic processes. Exosome degradation of rRNA, snoRNA, snRNA and tRNA in Saccharomyces cerevisiae is activated by TRAMP complexes, containing either the Trf4p or Trf5p poly(A) polymerase. These enzymes are presumed to facilitate exosome access by appending oligo(A)-tails onto structured substrates. Another role of the nuclear exosome is that of mRNA surveillance. In strains harboring a mutated THO/Sub2p system, involved in messenger ribonucleoprotein particle biogenesis and nuclear export, the exosome-associated 3' --> 5' exonuclease Rrp6p is required for both retention and degradation of nuclear restricted mRNAs. We show here that Trf4p, in the context of TRAMP, is an mRNA surveillance factor. However, unlike Rrp6p, Trf4p only partakes in RNA degradation and not in transcript retention. Surprisingly, a polyadenylation-defective Trf4p protein is fully active, suggesting polyadenylation-independent mRNA degradation. Transcription pulse-chase experiments show that HSP104 molecules undergoing quality control in THO/sub2 mutant strains fall into two distinct populations: One that is quickly degraded after transcription induction and another that escapes rapid decay and accumulates in foci associated with the HSP104 transcription site.

Authors+Show Affiliations

Centre National de la Recherche Scientifique, Centre de Genetique Moleculaire, Gif sur Yvette, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17410208

Citation

Rougemaille, Mathieu, et al. "Dissecting Mechanisms of Nuclear mRNA Surveillance in THO/sub2 Complex Mutants." The EMBO Journal, vol. 26, no. 9, 2007, pp. 2317-26.
Rougemaille M, Gudipati RK, Olesen JR, et al. Dissecting mechanisms of nuclear mRNA surveillance in THO/sub2 complex mutants. EMBO J. 2007;26(9):2317-26.
Rougemaille, M., Gudipati, R. K., Olesen, J. R., Thomsen, R., Seraphin, B., Libri, D., & Jensen, T. H. (2007). Dissecting mechanisms of nuclear mRNA surveillance in THO/sub2 complex mutants. The EMBO Journal, 26(9), 2317-26.
Rougemaille M, et al. Dissecting Mechanisms of Nuclear mRNA Surveillance in THO/sub2 Complex Mutants. EMBO J. 2007 May 2;26(9):2317-26. PubMed PMID: 17410208.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dissecting mechanisms of nuclear mRNA surveillance in THO/sub2 complex mutants. AU - Rougemaille,Mathieu, AU - Gudipati,Rajani Kanth, AU - Olesen,Jens Raabjerg, AU - Thomsen,Rune, AU - Seraphin,Bertrand, AU - Libri,Domenico, AU - Jensen,Torben Heick, Y1 - 2007/04/05/ PY - 2006/09/27/received PY - 2007/03/07/accepted PY - 2007/4/6/pubmed PY - 2007/7/13/medline PY - 2007/4/6/entrez SP - 2317 EP - 26 JF - The EMBO journal JO - EMBO J VL - 26 IS - 9 N2 - The nuclear exosome is involved in numerous RNA metabolic processes. Exosome degradation of rRNA, snoRNA, snRNA and tRNA in Saccharomyces cerevisiae is activated by TRAMP complexes, containing either the Trf4p or Trf5p poly(A) polymerase. These enzymes are presumed to facilitate exosome access by appending oligo(A)-tails onto structured substrates. Another role of the nuclear exosome is that of mRNA surveillance. In strains harboring a mutated THO/Sub2p system, involved in messenger ribonucleoprotein particle biogenesis and nuclear export, the exosome-associated 3' --> 5' exonuclease Rrp6p is required for both retention and degradation of nuclear restricted mRNAs. We show here that Trf4p, in the context of TRAMP, is an mRNA surveillance factor. However, unlike Rrp6p, Trf4p only partakes in RNA degradation and not in transcript retention. Surprisingly, a polyadenylation-defective Trf4p protein is fully active, suggesting polyadenylation-independent mRNA degradation. Transcription pulse-chase experiments show that HSP104 molecules undergoing quality control in THO/sub2 mutant strains fall into two distinct populations: One that is quickly degraded after transcription induction and another that escapes rapid decay and accumulates in foci associated with the HSP104 transcription site. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/17410208/Dissecting_mechanisms_of_nuclear_mRNA_surveillance_in_THO/sub2_complex_mutants_ L2 - https://doi.org/10.1038/sj.emboj.7601669 DB - PRIME DP - Unbound Medicine ER -