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Effects of calcitonin and parathyroid hormone on the regulation of cabindin-D(9k) in the uterus, placenta, and fetal membrane of rats related to blood calcium level during late gestation.

Abstract

Calbindin-D(9k) (CaBP-9k) gene is expressed in the uterus of pregnant rats, which is regulated by steroid hormones during estrous cycle or gestation. We hypothesized that there is a positive correlation between altered CaBP-9k expression and change in one or more of the hormones to provide a clue to the mechanism responsible for the altered calcium levels in the uterus, placenta, and fetal membrane during late gestation. Thus, in the present study, we investigated the effects of the hormones including estradiol (E2), calcitonin (CT), and parathyroid hormone (PTH) on the regulation of CaBP-9k in these tissues. There was an increase in the level of CaBP-9k in the uterus, placenta, and extra-embryonic membrane at late gestation, as blood calcium level increased. The protein level of CaBP-9k remained lower in the uterus at two-thirds of pregnancy, and then it rebounded abruptly during late pregnancy. During late gestation, E2 is postulated to be a dominant factor in the regulation of uterine CaBP-9k gene expression. Furthermore, we assumed that there is a positive correlation between altered expression of CaBP-9k and blood calcium level during pregnancy. The present study demonstrated the regulation of CaBP-9k mRNA in the uterus, placenta, and fetal membrane of rats, implying a role for CaBP-9k gene in the control of blood calcium in placenta and the calcium passing from maternal blood to fetal circulation. Taken together, these results suggest that major alterations in calcium metabolism caused by maternal thyroparathyroidectomy (TPTX), are sufficient to affect the changes in reproductive tissues during late pregnancy. In addition, an increase of blood calcium level is one of the most significant factors in the regulation of CaBP-9k at the transcriptional and/or translational levels in the reproductive tissues during late pregnancy.

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  • Authors+Show Affiliations

    ,

    Laboratory of Veterinary Biochemistry and Molecular Biotechnology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.

    , ,

    Source

    MeSH

    Animals
    Calbindins
    Calcitonin
    Calcium
    Estradiol
    Extraembryonic Membranes
    Female
    Gene Expression
    Gene Expression Regulation
    Gonadal Steroid Hormones
    Hormone Antagonists
    Parathyroid Hormone
    Placenta
    Pregnancy
    Pregnancy, Animal
    Protein Biosynthesis
    Rats
    Rats, Sprague-Dawley
    S100 Calcium Binding Protein G
    Uterus

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    17410547

    Citation

    Hong, Eui-Ju, et al. "Effects of Calcitonin and Parathyroid Hormone On the Regulation of cabindin-D(9k) in the Uterus, Placenta, and Fetal Membrane of Rats Related to Blood Calcium Level During Late Gestation." Molecular Reproduction and Development, vol. 74, no. 9, 2007, pp. 1188-97.
    Hong EJ, Choi KC, Hyun SH, et al. Effects of calcitonin and parathyroid hormone on the regulation of cabindin-D(9k) in the uterus, placenta, and fetal membrane of rats related to blood calcium level during late gestation. Mol Reprod Dev. 2007;74(9):1188-97.
    Hong, E. J., Choi, K. C., Hyun, S. H., & Jeung, E. B. (2007). Effects of calcitonin and parathyroid hormone on the regulation of cabindin-D(9k) in the uterus, placenta, and fetal membrane of rats related to blood calcium level during late gestation. Molecular Reproduction and Development, 74(9), pp. 1188-97.
    Hong EJ, et al. Effects of Calcitonin and Parathyroid Hormone On the Regulation of cabindin-D(9k) in the Uterus, Placenta, and Fetal Membrane of Rats Related to Blood Calcium Level During Late Gestation. Mol Reprod Dev. 2007;74(9):1188-97. PubMed PMID: 17410547.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effects of calcitonin and parathyroid hormone on the regulation of cabindin-D(9k) in the uterus, placenta, and fetal membrane of rats related to blood calcium level during late gestation. AU - Hong,Eui-Ju, AU - Choi,Kyung-Chul, AU - Hyun,Sang-Hwan, AU - Jeung,Eui-Bae, PY - 2007/4/6/pubmed PY - 2007/12/6/medline PY - 2007/4/6/entrez SP - 1188 EP - 97 JF - Molecular reproduction and development JO - Mol. Reprod. Dev. VL - 74 IS - 9 N2 - Calbindin-D(9k) (CaBP-9k) gene is expressed in the uterus of pregnant rats, which is regulated by steroid hormones during estrous cycle or gestation. We hypothesized that there is a positive correlation between altered CaBP-9k expression and change in one or more of the hormones to provide a clue to the mechanism responsible for the altered calcium levels in the uterus, placenta, and fetal membrane during late gestation. Thus, in the present study, we investigated the effects of the hormones including estradiol (E2), calcitonin (CT), and parathyroid hormone (PTH) on the regulation of CaBP-9k in these tissues. There was an increase in the level of CaBP-9k in the uterus, placenta, and extra-embryonic membrane at late gestation, as blood calcium level increased. The protein level of CaBP-9k remained lower in the uterus at two-thirds of pregnancy, and then it rebounded abruptly during late pregnancy. During late gestation, E2 is postulated to be a dominant factor in the regulation of uterine CaBP-9k gene expression. Furthermore, we assumed that there is a positive correlation between altered expression of CaBP-9k and blood calcium level during pregnancy. The present study demonstrated the regulation of CaBP-9k mRNA in the uterus, placenta, and fetal membrane of rats, implying a role for CaBP-9k gene in the control of blood calcium in placenta and the calcium passing from maternal blood to fetal circulation. Taken together, these results suggest that major alterations in calcium metabolism caused by maternal thyroparathyroidectomy (TPTX), are sufficient to affect the changes in reproductive tissues during late pregnancy. In addition, an increase of blood calcium level is one of the most significant factors in the regulation of CaBP-9k at the transcriptional and/or translational levels in the reproductive tissues during late pregnancy. SN - 1040-452X UR - https://www.unboundmedicine.com/medline/citation/17410547/Effects_of_calcitonin_and_parathyroid_hormone_on_the_regulation_of_cabindin-D(9k)_in_the_uterus,_placenta,_and_fetal_membrane_of_rats_related_to_blood_calcium_level_during_late_gestation L2 - https://doi.org/10.1002/mrd.20627 DB - PRIME DP - Unbound Medicine ER -