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In-capillary derivatization and laser-induced fluorescence detection for the analysis of organophosphorus pesticides by micellar electrokinetic chromatography.
J Chromatogr A. 2007 May 18; 1149(2):377-84.JC

Abstract

We developed a rapid and sensitive method using in-capillary derivatization and laser-induced fluorescence (LIF) detection for the fully automated analysis of organophosphorus pesticides (OPPs), including glufosinate, aminomethylphosphonic acid (AMPA) and glyphosate by micellar electrokinetic chromatography (MEKC). The potential of 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) as in-capillary derivatization reagent is described for the first time. The unique feature of this MEKC method is the capillary being used as a small reaction chamber. In in-capillary derivatization, the sample and reagent solutions were injected directly into the capillary by tandem mode, followed by an electrokinetic step to enhance the mixing efficiency of analytes and reagent plugs in accordance with their different electrophoretic mobilities. Standing a specified time for reaction, the derivatives were then immediately separated and determined. Careful optimization of the derivatization and separation conditions allowed the determination of glufosinate, AMPA and glyphosate with detection limits of 2.8, 3.6 and 32.2 ng/mL, respectively. These detection limits were comparable to those of 1.4, 1.9 and 23.8 ng/mL obtained from conventional pre-capillary derivatization. Furthermore, repeatability better than 0.40% for migration time and 3.4% for peak area, as well as shorter migration time, was obtained. The method was successfully applied to the analysis of spiked river water sample with satisfactory results.

Authors+Show Affiliations

Department of Chemistry, Lanzhou University, Lanzhou 730000, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

17412355

Citation

Zhou, Lei, et al. "In-capillary Derivatization and Laser-induced Fluorescence Detection for the Analysis of Organophosphorus Pesticides By Micellar Electrokinetic Chromatography." Journal of Chromatography. A, vol. 1149, no. 2, 2007, pp. 377-84.
Zhou L, Luo Z, Wang S, et al. In-capillary derivatization and laser-induced fluorescence detection for the analysis of organophosphorus pesticides by micellar electrokinetic chromatography. J Chromatogr A. 2007;1149(2):377-84.
Zhou, L., Luo, Z., Wang, S., Hui, Y., Hu, Z., & Chen, X. (2007). In-capillary derivatization and laser-induced fluorescence detection for the analysis of organophosphorus pesticides by micellar electrokinetic chromatography. Journal of Chromatography. A, 1149(2), 377-84.
Zhou L, et al. In-capillary Derivatization and Laser-induced Fluorescence Detection for the Analysis of Organophosphorus Pesticides By Micellar Electrokinetic Chromatography. J Chromatogr A. 2007 May 18;1149(2):377-84. PubMed PMID: 17412355.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In-capillary derivatization and laser-induced fluorescence detection for the analysis of organophosphorus pesticides by micellar electrokinetic chromatography. AU - Zhou,Lei, AU - Luo,Zhi, AU - Wang,Shumin, AU - Hui,Yang, AU - Hu,Zhide, AU - Chen,Xingguo, Y1 - 2007/03/16/ PY - 2006/11/13/received PY - 2007/02/26/revised PY - 2007/03/05/accepted PY - 2007/4/7/pubmed PY - 2007/6/22/medline PY - 2007/4/7/entrez SP - 377 EP - 84 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1149 IS - 2 N2 - We developed a rapid and sensitive method using in-capillary derivatization and laser-induced fluorescence (LIF) detection for the fully automated analysis of organophosphorus pesticides (OPPs), including glufosinate, aminomethylphosphonic acid (AMPA) and glyphosate by micellar electrokinetic chromatography (MEKC). The potential of 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) as in-capillary derivatization reagent is described for the first time. The unique feature of this MEKC method is the capillary being used as a small reaction chamber. In in-capillary derivatization, the sample and reagent solutions were injected directly into the capillary by tandem mode, followed by an electrokinetic step to enhance the mixing efficiency of analytes and reagent plugs in accordance with their different electrophoretic mobilities. Standing a specified time for reaction, the derivatives were then immediately separated and determined. Careful optimization of the derivatization and separation conditions allowed the determination of glufosinate, AMPA and glyphosate with detection limits of 2.8, 3.6 and 32.2 ng/mL, respectively. These detection limits were comparable to those of 1.4, 1.9 and 23.8 ng/mL obtained from conventional pre-capillary derivatization. Furthermore, repeatability better than 0.40% for migration time and 3.4% for peak area, as well as shorter migration time, was obtained. The method was successfully applied to the analysis of spiked river water sample with satisfactory results. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17412355/In_capillary_derivatization_and_laser_induced_fluorescence_detection_for_the_analysis_of_organophosphorus_pesticides_by_micellar_electrokinetic_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)00466-9 DB - PRIME DP - Unbound Medicine ER -