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Dengue viruses can infect human primary lung epithelia as well as lung carcinoma cells, and can also induce the secretion of IL-6 and RANTES.
Virus Res. 2007 Jun; 126(1-2):216-25.VR

Abstract

Dengue viruses (DENV) are herein demonstrated for the first time as being able to infect and replicate in human primary lung epithelium and various lung cancer cell lines. The detection of dengue virus particles and viral negative strand RNA synthesis in the cell, in conjunction with the release of viral progenies in culture supernatants, support the notion that lung cells are susceptible to dengue virus infection. The replication efficiency of DENV in lung cancer cells from high to low is: DEN-2 (dengue virus type-2), DEN-3, DEN-4 and DEN-1. Moreover, the susceptibility of the six lung cancer cell lines to DEN-2 infection is: SW1573>A549>H1435; H23; H520; Bes2B. DEN-2 infection significantly increased the expression levels of IL-6 and RANTES in four of the six lung cancer cell lines, which is consistent with the high expression levels of these molecules in DHF/DSS patients. IL-6 expression induced by DEN-2 infection was NF-kappaB dependent. In summary, our results indicate that lung epithelial cell is a possible target of dengue viruses and IL-6 and RANTES may play pivotal roles in lung related immuno-pathogenesis.

Authors+Show Affiliations

Lee YR
Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC.
Su CY
No affiliation info available
Chow NH
No affiliation info available
Lai WW
No affiliation info available
Lei HY
No affiliation info available
Chang CL
No affiliation info available
Chang TY
No affiliation info available
Chen SH
No affiliation info available
Lin YS
No affiliation info available
Yeh TM
No affiliation info available
Liu HS
No affiliation info available

MeSH

Base SequenceCell Line, TumorCells, CulturedChemokine CCL5Dengue VirusEpithelial CellsHumansInterleukin-6LungLung NeoplasmsNF-kappa BPromoter Regions, GeneticRNA, ViralVirus Replication

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17416433

Citation

Lee, Ying-Ray, et al. "Dengue Viruses Can Infect Human Primary Lung Epithelia as Well as Lung Carcinoma Cells, and Can Also Induce the Secretion of IL-6 and RANTES." Virus Research, vol. 126, no. 1-2, 2007, pp. 216-25.
Lee YR, Su CY, Chow NH, et al. Dengue viruses can infect human primary lung epithelia as well as lung carcinoma cells, and can also induce the secretion of IL-6 and RANTES. Virus Res. 2007;126(1-2):216-25.
Lee, Y. R., Su, C. Y., Chow, N. H., Lai, W. W., Lei, H. Y., Chang, C. L., Chang, T. Y., Chen, S. H., Lin, Y. S., Yeh, T. M., & Liu, H. S. (2007). Dengue viruses can infect human primary lung epithelia as well as lung carcinoma cells, and can also induce the secretion of IL-6 and RANTES. Virus Research, 126(1-2), 216-25.
Lee YR, et al. Dengue Viruses Can Infect Human Primary Lung Epithelia as Well as Lung Carcinoma Cells, and Can Also Induce the Secretion of IL-6 and RANTES. Virus Res. 2007;126(1-2):216-25. PubMed PMID: 17416433.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dengue viruses can infect human primary lung epithelia as well as lung carcinoma cells, and can also induce the secretion of IL-6 and RANTES. AU - Lee,Ying-Ray, AU - Su,Ching-Yao, AU - Chow,Nan-Haw, AU - Lai,Wu-Wei, AU - Lei,Huan-Yao, AU - Chang,Chia-Lun, AU - Chang,Tsuey-Yu, AU - Chen,Shun-Hua, AU - Lin,Yee-Shin, AU - Yeh,Trai-Ming, AU - Liu,Hsiao-Sheng, Y1 - 2007/04/09/ PY - 2006/10/11/received PY - 2007/03/06/revised PY - 2007/03/08/accepted PY - 2007/4/10/pubmed PY - 2007/8/19/medline PY - 2007/4/10/entrez SP - 216 EP - 25 JF - Virus research JO - Virus Res VL - 126 IS - 1-2 N2 - Dengue viruses (DENV) are herein demonstrated for the first time as being able to infect and replicate in human primary lung epithelium and various lung cancer cell lines. The detection of dengue virus particles and viral negative strand RNA synthesis in the cell, in conjunction with the release of viral progenies in culture supernatants, support the notion that lung cells are susceptible to dengue virus infection. The replication efficiency of DENV in lung cancer cells from high to low is: DEN-2 (dengue virus type-2), DEN-3, DEN-4 and DEN-1. Moreover, the susceptibility of the six lung cancer cell lines to DEN-2 infection is: SW1573>A549>H1435; H23; H520; Bes2B. DEN-2 infection significantly increased the expression levels of IL-6 and RANTES in four of the six lung cancer cell lines, which is consistent with the high expression levels of these molecules in DHF/DSS patients. IL-6 expression induced by DEN-2 infection was NF-kappaB dependent. In summary, our results indicate that lung epithelial cell is a possible target of dengue viruses and IL-6 and RANTES may play pivotal roles in lung related immuno-pathogenesis. SN - 0168-1702 UR - https://www.unboundmedicine.com/medline/citation/17416433/Dengue_viruses_can_infect_human_primary_lung_epithelia_as_well_as_lung_carcinoma_cells_and_can_also_induce_the_secretion_of_IL_6_and_RANTES_ DB - PRIME DP - Unbound Medicine ER -