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Headspace liquid-phase microextraction of methamphetamine and amphetamine in urine by an aqueous drop.
Anal Chim Acta. 2007 Apr 25; 589(2):225-30.AC

Abstract

This study developed a headspace liquid-phase microextraction (LPME) method by using a single aqueous drop in combination with high performance liquid chromatography (HPLC)-UV detection for the determination of methamphetamine (MAP) and amphetamine (AP) in urine samples. The analytes, volatile and basic, were released from sample matrix into the headspace first, and then protonated and dissolved in an aqueous H(3)PO(4) drop hanging in the headspace by a HPLC syringe. After extraction, this drop was directly injected into HPLC. Parameters affecting extraction efficiency were investigated and optimized. This method showed good linearity in the investigated concentration range of 1.0-1500 microg L(-1), repeatability of the extraction (R.S.D.<5%, n=6), and low detection limits (0.3 microg L(-1) for both analytes). Enrichment factors of about 400-fold and 220-fold were achieved for MAP and AP, respectively, at optimum conditions. The feasibility of the method was demonstrated by analyzing human urine samples.

Authors+Show Affiliations

Department of Sciences, John Jay College of Criminal Justice, The City University of New York, New York, NY 10019, USA. yhe@jjay.cuny.edu <yhe@jjay.cuny.edu>No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17418185

Citation

He, Yi, et al. "Headspace Liquid-phase Microextraction of Methamphetamine and Amphetamine in Urine By an Aqueous Drop." Analytica Chimica Acta, vol. 589, no. 2, 2007, pp. 225-30.
He Y, Vargas A, Kang YJ. Headspace liquid-phase microextraction of methamphetamine and amphetamine in urine by an aqueous drop. Anal Chim Acta. 2007;589(2):225-30.
He, Y., Vargas, A., & Kang, Y. J. (2007). Headspace liquid-phase microextraction of methamphetamine and amphetamine in urine by an aqueous drop. Analytica Chimica Acta, 589(2), 225-30.
He Y, Vargas A, Kang YJ. Headspace Liquid-phase Microextraction of Methamphetamine and Amphetamine in Urine By an Aqueous Drop. Anal Chim Acta. 2007 Apr 25;589(2):225-30. PubMed PMID: 17418185.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Headspace liquid-phase microextraction of methamphetamine and amphetamine in urine by an aqueous drop. AU - He,Yi, AU - Vargas,Angelica, AU - Kang,Youn-Jung, Y1 - 2007/03/04/ PY - 2006/11/28/received PY - 2007/02/23/revised PY - 2007/02/23/accepted PY - 2007/4/10/pubmed PY - 2007/6/20/medline PY - 2007/4/10/entrez SP - 225 EP - 30 JF - Analytica chimica acta JO - Anal Chim Acta VL - 589 IS - 2 N2 - This study developed a headspace liquid-phase microextraction (LPME) method by using a single aqueous drop in combination with high performance liquid chromatography (HPLC)-UV detection for the determination of methamphetamine (MAP) and amphetamine (AP) in urine samples. The analytes, volatile and basic, were released from sample matrix into the headspace first, and then protonated and dissolved in an aqueous H(3)PO(4) drop hanging in the headspace by a HPLC syringe. After extraction, this drop was directly injected into HPLC. Parameters affecting extraction efficiency were investigated and optimized. This method showed good linearity in the investigated concentration range of 1.0-1500 microg L(-1), repeatability of the extraction (R.S.D.<5%, n=6), and low detection limits (0.3 microg L(-1) for both analytes). Enrichment factors of about 400-fold and 220-fold were achieved for MAP and AP, respectively, at optimum conditions. The feasibility of the method was demonstrated by analyzing human urine samples. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/17418185/Headspace_liquid_phase_microextraction_of_methamphetamine_and_amphetamine_in_urine_by_an_aqueous_drop_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(07)00435-7 DB - PRIME DP - Unbound Medicine ER -