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Immune responses of pigs inoculated with a recombinant fowlpox virus coexpressing GP5/GP3 of porcine reproductive and respiratory syndrome virus and swine IL-18.
Vaccine. 2007 May 22; 25(21):4193-202.V

Abstract

Two recombinant fowlpox viruses (rFPV-ORF5-ORF3 and rFPV-IL-18-ORF5-ORF3) containing the ORF5/ORF3 cDNAs of PRRSV (strain Chang Chun) and IL-18 of swine were constructed and evaluated for theirs abilities to induce humoral and cellular responses in piglets. In addition, their abilities to protect piglets against homologous virus challenge were examined. All piglets were given booster vaccinations at 21 days after the initial inoculation, and all piglets were challenged at 60 after the initial inoculation. Control groups were inoculated with wild-type fowlpox virus (wtFPV). All animals vaccinated with rFPV-ORF5-ORF3 and rFPV-IL-18-ORF5-ORF3 developed specific anti-PRRSV ELISA antibody and neutralizing antibody, as well as T-lymphocyte proliferation response. To evaluate the cellular immune function, IFN-gamma production in pigs serum and T-lymphocytes (CD4 and CD8 T cells) in peripheral blood were examined. Following challenge with a pathogenic strain of PRRSV (strain Chang Chun), piglets inoculated with recombinant fowlpox virus (rFPV) showed lower (P<0.05) temperature, viremia and virus load in bronchial lymph nodes than control animals, suggesting the establishment of partial protection against PRRSV infection. The results demonstrated the potential use of a fowlpox virus-based recombinant vaccine in the control and prevention of PRRSV infections.

Authors+Show Affiliations

Genetic Engineering Laboratory, Academy of Military Medical Sciences, Changchun 130062, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17418456

Citation

Shen, Guoshun, et al. "Immune Responses of Pigs Inoculated With a Recombinant Fowlpox Virus Coexpressing GP5/GP3 of Porcine Reproductive and Respiratory Syndrome Virus and Swine IL-18." Vaccine, vol. 25, no. 21, 2007, pp. 4193-202.
Shen G, Jin N, Ma M, et al. Immune responses of pigs inoculated with a recombinant fowlpox virus coexpressing GP5/GP3 of porcine reproductive and respiratory syndrome virus and swine IL-18. Vaccine. 2007;25(21):4193-202.
Shen, G., Jin, N., Ma, M., Jin, K., Zheng, M., Zhuang, T., Lu, H., Zhu, G., Jin, H., Jin, M., Huo, X., Qin, X., Yin, R., Li, C., Li, H., Li, Y., Han, Z., Chen, Y., & Jin, M. (2007). Immune responses of pigs inoculated with a recombinant fowlpox virus coexpressing GP5/GP3 of porcine reproductive and respiratory syndrome virus and swine IL-18. Vaccine, 25(21), 4193-202.
Shen G, et al. Immune Responses of Pigs Inoculated With a Recombinant Fowlpox Virus Coexpressing GP5/GP3 of Porcine Reproductive and Respiratory Syndrome Virus and Swine IL-18. Vaccine. 2007 May 22;25(21):4193-202. PubMed PMID: 17418456.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immune responses of pigs inoculated with a recombinant fowlpox virus coexpressing GP5/GP3 of porcine reproductive and respiratory syndrome virus and swine IL-18. AU - Shen,Guoshun, AU - Jin,Ningyi, AU - Ma,Mingxiao, AU - Jin,Kuoshi, AU - Zheng,Min, AU - Zhuang,Tianzhong, AU - Lu,Huijun, AU - Zhu,Guangze, AU - Jin,Hongtao, AU - Jin,Minglan, AU - Huo,Xiaowei, AU - Qin,Xiaoguang, AU - Yin,Ronglan, AU - Li,Chang, AU - Li,Hongwen, AU - Li,Yang, AU - Han,Zhenzhen, AU - Chen,Yifeng, AU - Jin,Miaomiao, Y1 - 2007/03/22/ PY - 2006/12/12/received PY - 2007/02/23/revised PY - 2007/03/02/accepted PY - 2007/4/10/pubmed PY - 2007/8/21/medline PY - 2007/4/10/entrez SP - 4193 EP - 202 JF - Vaccine JO - Vaccine VL - 25 IS - 21 N2 - Two recombinant fowlpox viruses (rFPV-ORF5-ORF3 and rFPV-IL-18-ORF5-ORF3) containing the ORF5/ORF3 cDNAs of PRRSV (strain Chang Chun) and IL-18 of swine were constructed and evaluated for theirs abilities to induce humoral and cellular responses in piglets. In addition, their abilities to protect piglets against homologous virus challenge were examined. All piglets were given booster vaccinations at 21 days after the initial inoculation, and all piglets were challenged at 60 after the initial inoculation. Control groups were inoculated with wild-type fowlpox virus (wtFPV). All animals vaccinated with rFPV-ORF5-ORF3 and rFPV-IL-18-ORF5-ORF3 developed specific anti-PRRSV ELISA antibody and neutralizing antibody, as well as T-lymphocyte proliferation response. To evaluate the cellular immune function, IFN-gamma production in pigs serum and T-lymphocytes (CD4 and CD8 T cells) in peripheral blood were examined. Following challenge with a pathogenic strain of PRRSV (strain Chang Chun), piglets inoculated with recombinant fowlpox virus (rFPV) showed lower (P<0.05) temperature, viremia and virus load in bronchial lymph nodes than control animals, suggesting the establishment of partial protection against PRRSV infection. The results demonstrated the potential use of a fowlpox virus-based recombinant vaccine in the control and prevention of PRRSV infections. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/17418456/Immune_responses_of_pigs_inoculated_with_a_recombinant_fowlpox_virus_coexpressing_GP5/GP3_of_porcine_reproductive_and_respiratory_syndrome_virus_and_swine_IL_18_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(07)00298-8 DB - PRIME DP - Unbound Medicine ER -