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Effect of PPAR-delta agonist on the expression of visfatin, adiponectin, and resistin in rat adipose tissue and 3T3-L1 adipocytes.
Biochem Biophys Res Commun. 2007 May 25; 357(1):62-7.BB

Abstract

It has been recently reported that activation of PPAR-delta, by specific agonists or genetic manipulation, alleviates dyslipidemia, hyperglycemia, and insulin resistance in animal models of obesity and type 2 diabetes. The purpose of the present study was to determine whether the PPAR-delta agonist has a direct effect on adipokines in visceral adipose tissue of rats and in cultured adipocytes. We examined the expression of visfatin, adiponectin, and resistin mRNA in visceral adipose tissue of Wistar rats fed a high-fat diet and 3T3-L1 adipocytes treated with PPAR-delta agonist (L-165041). Body weight and biochemical measurements were performed. Rats fed a high-fat diet showed a greater increase in body weight than those fed a standard diet (P<0.05), and treatment with L-165041 (10 mg/kg/day) significantly decreased weight gain (P<0.05). The concentration of total cholesterol was lower, and HDL cholesterol was higher in L-165041-treated rats (P<0.05). In the visceral adipose tissue of L-165041-treated rats, visfatin and adiponectin mRNA levels significantly increased compared to those of the untreated rats (P<0.05). However, the expression of resistin decreased in the L-165041-treated rats. Furthermore, in cultured 3T3-L1 adipocytes, the level of visfatin and adiponectin mRNA was up-regulated in response to L-165041 treatment for nine days. By contrast, resistin mRNA levels were down-regulated by L-165041 treatment. The present study provides a novel evidence to suggest that the PPAR-delta agonist has regulatory effects on a variety of adipokines, and these effects might explain some of their metabolic function.

Authors+Show Affiliations

Division of Endocrinology and Metabolism, Department of Internal Medicine, Korea University College of Medicine, Guro-Gu, Seoul 152-050, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17418807

Citation

Choi, K C., et al. "Effect of PPAR-delta Agonist On the Expression of Visfatin, Adiponectin, and Resistin in Rat Adipose Tissue and 3T3-L1 Adipocytes." Biochemical and Biophysical Research Communications, vol. 357, no. 1, 2007, pp. 62-7.
Choi KC, Lee SY, Yoo HJ, et al. Effect of PPAR-delta agonist on the expression of visfatin, adiponectin, and resistin in rat adipose tissue and 3T3-L1 adipocytes. Biochem Biophys Res Commun. 2007;357(1):62-7.
Choi, K. C., Lee, S. Y., Yoo, H. J., Ryu, O. H., Lee, K. W., Kim, S. M., Baik, S. H., & Choi, K. M. (2007). Effect of PPAR-delta agonist on the expression of visfatin, adiponectin, and resistin in rat adipose tissue and 3T3-L1 adipocytes. Biochemical and Biophysical Research Communications, 357(1), 62-7.
Choi KC, et al. Effect of PPAR-delta Agonist On the Expression of Visfatin, Adiponectin, and Resistin in Rat Adipose Tissue and 3T3-L1 Adipocytes. Biochem Biophys Res Commun. 2007 May 25;357(1):62-7. PubMed PMID: 17418807.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of PPAR-delta agonist on the expression of visfatin, adiponectin, and resistin in rat adipose tissue and 3T3-L1 adipocytes. AU - Choi,K C, AU - Lee,S Y, AU - Yoo,H J, AU - Ryu,O H, AU - Lee,K W, AU - Kim,S M, AU - Baik,S H, AU - Choi,K M, Y1 - 2007/03/28/ PY - 2007/03/06/received PY - 2007/03/12/accepted PY - 2007/4/10/pubmed PY - 2007/6/15/medline PY - 2007/4/10/entrez SP - 62 EP - 7 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 357 IS - 1 N2 - It has been recently reported that activation of PPAR-delta, by specific agonists or genetic manipulation, alleviates dyslipidemia, hyperglycemia, and insulin resistance in animal models of obesity and type 2 diabetes. The purpose of the present study was to determine whether the PPAR-delta agonist has a direct effect on adipokines in visceral adipose tissue of rats and in cultured adipocytes. We examined the expression of visfatin, adiponectin, and resistin mRNA in visceral adipose tissue of Wistar rats fed a high-fat diet and 3T3-L1 adipocytes treated with PPAR-delta agonist (L-165041). Body weight and biochemical measurements were performed. Rats fed a high-fat diet showed a greater increase in body weight than those fed a standard diet (P<0.05), and treatment with L-165041 (10 mg/kg/day) significantly decreased weight gain (P<0.05). The concentration of total cholesterol was lower, and HDL cholesterol was higher in L-165041-treated rats (P<0.05). In the visceral adipose tissue of L-165041-treated rats, visfatin and adiponectin mRNA levels significantly increased compared to those of the untreated rats (P<0.05). However, the expression of resistin decreased in the L-165041-treated rats. Furthermore, in cultured 3T3-L1 adipocytes, the level of visfatin and adiponectin mRNA was up-regulated in response to L-165041 treatment for nine days. By contrast, resistin mRNA levels were down-regulated by L-165041 treatment. The present study provides a novel evidence to suggest that the PPAR-delta agonist has regulatory effects on a variety of adipokines, and these effects might explain some of their metabolic function. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/17418807/Effect_of_PPAR_delta_agonist_on_the_expression_of_visfatin_adiponectin_and_resistin_in_rat_adipose_tissue_and_3T3_L1_adipocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(07)00549-9 DB - PRIME DP - Unbound Medicine ER -