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Long-term potentiation of neuronal excitation by neuron-glia interactions in the rat spinal dorsal horn.
Eur J Neurosci. 2007 Mar; 25(5):1297-306.EJ

Abstract

By imaging neuronal excitation in rat spinal cord slices with a voltage-sensitive dye, we examined the role of glial cells in the P2X receptor agonist alphabeta-methylene ATP (alphabetameATP)-triggered long-term potentiation (LTP) in the dorsal horn. Bath application of alphabetameATP potentiated neuronal excitation in the superficial dorsal horn. The potentiation was inhibited in the presence of the P2X receptor antagonists TNP-ATP, PPADS and A-317491, and was not induced in slices taken from rats neonatally treated with capsaicin. These results suggest that alphabetameATP acts on P2X receptors, possibly P2X(3) and/or P2X(2/3), in capsaicin-sensitive primary afferent terminals. Furthermore, the potentiation was inhibited by treatment with the glial metabolism inhibitor monofluoroacetic acid. Results obtained with the p38 mitogen-activated protein kinase (p38 MAPK) inhibitor SB203580, tumour necrosis factor-alpha (TNF-alpha) and interleukin (IL)-6, and antibodies to TNF-alpha and IL-6, as well as by double immunolabelling of activated p38 MAPK with markers of astrocytes and microglia, demonstrated that alphabetameATP activated p38 MAPK in astrocytes, and that the presence of proinflammatory cytokines and p38 MAPK activation were necessary for the induction of alphabetameATP-triggered LTP. These findings indicate that glial cells contribute to the alphabetameATP-induced LTP, which might be part of a cellular mechanism for the induction of persistent pain.

Authors+Show Affiliations

Department of Human and Artificial Intelligence Systems, Graduate School of Engineering, and Research and Education Program for Life Science, University of Fukui, 3-9-1 Bunkyo, Fukui 910-8507, Japan. ikeda@synapse.his.fukui-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17425556

Citation

Ikeda, Hiroshi, et al. "Long-term Potentiation of Neuronal Excitation By Neuron-glia Interactions in the Rat Spinal Dorsal Horn." The European Journal of Neuroscience, vol. 25, no. 5, 2007, pp. 1297-306.
Ikeda H, Tsuda M, Inoue K, et al. Long-term potentiation of neuronal excitation by neuron-glia interactions in the rat spinal dorsal horn. Eur J Neurosci. 2007;25(5):1297-306.
Ikeda, H., Tsuda, M., Inoue, K., & Murase, K. (2007). Long-term potentiation of neuronal excitation by neuron-glia interactions in the rat spinal dorsal horn. The European Journal of Neuroscience, 25(5), 1297-306.
Ikeda H, et al. Long-term Potentiation of Neuronal Excitation By Neuron-glia Interactions in the Rat Spinal Dorsal Horn. Eur J Neurosci. 2007;25(5):1297-306. PubMed PMID: 17425556.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long-term potentiation of neuronal excitation by neuron-glia interactions in the rat spinal dorsal horn. AU - Ikeda,Hiroshi, AU - Tsuda,Makoto, AU - Inoue,Kazuhide, AU - Murase,Kazuyuki, PY - 2007/4/12/pubmed PY - 2007/6/26/medline PY - 2007/4/12/entrez SP - 1297 EP - 306 JF - The European journal of neuroscience JO - Eur. J. Neurosci. VL - 25 IS - 5 N2 - By imaging neuronal excitation in rat spinal cord slices with a voltage-sensitive dye, we examined the role of glial cells in the P2X receptor agonist alphabeta-methylene ATP (alphabetameATP)-triggered long-term potentiation (LTP) in the dorsal horn. Bath application of alphabetameATP potentiated neuronal excitation in the superficial dorsal horn. The potentiation was inhibited in the presence of the P2X receptor antagonists TNP-ATP, PPADS and A-317491, and was not induced in slices taken from rats neonatally treated with capsaicin. These results suggest that alphabetameATP acts on P2X receptors, possibly P2X(3) and/or P2X(2/3), in capsaicin-sensitive primary afferent terminals. Furthermore, the potentiation was inhibited by treatment with the glial metabolism inhibitor monofluoroacetic acid. Results obtained with the p38 mitogen-activated protein kinase (p38 MAPK) inhibitor SB203580, tumour necrosis factor-alpha (TNF-alpha) and interleukin (IL)-6, and antibodies to TNF-alpha and IL-6, as well as by double immunolabelling of activated p38 MAPK with markers of astrocytes and microglia, demonstrated that alphabetameATP activated p38 MAPK in astrocytes, and that the presence of proinflammatory cytokines and p38 MAPK activation were necessary for the induction of alphabetameATP-triggered LTP. These findings indicate that glial cells contribute to the alphabetameATP-induced LTP, which might be part of a cellular mechanism for the induction of persistent pain. SN - 0953-816X UR - https://www.unboundmedicine.com/medline/citation/17425556/Long_term_potentiation_of_neuronal_excitation_by_neuron_glia_interactions_in_the_rat_spinal_dorsal_horn_ L2 - https://doi.org/10.1111/j.1460-9568.2007.05386.x DB - PRIME DP - Unbound Medicine ER -