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Central carbon metabolism of Saccharomyces cerevisiae in anaerobic, oxygen-limited and fully aerobic steady-state conditions and following a shift to anaerobic conditions.
FEMS Yeast Res. 2008 Feb; 8(1):140-54.FY

Abstract

Saccharomyces cerevisiae CEN.PK113-1A was grown in glucose-limited chemostat culture with 0%, 0.5%, 1.0%, 2.8% or 20.9% O2 in the inlet gas (D=0.10 h(-1), pH 5, 30 degrees C) to determine the effects of oxygen on 17 metabolites and 69 genes related to central carbon metabolism. The concentrations of tricarboxylic acid cycle (TCA) metabolites and all glycolytic metabolites except 2-phosphoglycerate+3-phosphoglycerate and phosphoenolpyruvate were higher in anaerobic than in fully aerobic conditions. Provision of only 0.5-1% O2 reduced the concentrations of most metabolites, as compared with anaerobic conditions. Transcription of most genes analyzed was reduced in 0%, 0.5% or 1.0% O2 relative to cells grown in 2.8% or 20.9% O2. Ethanol production was observed with 2.8% or less O2. After steady-state analysis in defined oxygen concentrations, the conditions were switched from aerobic to anaerobic. Metabolite and transcript levels were monitored for up to 96 h after the transition, and this showed that more than 30 h was required for the cells to fully adapt to anaerobiosis. Levels of metabolites of upper glycolysis and the TCA cycle increased following the transition to anaerobic conditions, whereas those of metabolites of lower glycolysis generally decreased. Gene regulation was more complex, with some genes showing transient upregulation or downregulation during the adaptation to anaerobic conditions.

Authors+Show Affiliations

VTT Technical Research Centre of Finland, Finland. marilyn.wiebe@vtt.fiNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17425669

Citation

Wiebe, Marilyn G., et al. "Central Carbon Metabolism of Saccharomyces Cerevisiae in Anaerobic, Oxygen-limited and Fully Aerobic Steady-state Conditions and Following a Shift to Anaerobic Conditions." FEMS Yeast Research, vol. 8, no. 1, 2008, pp. 140-54.
Wiebe MG, Rintala E, Tamminen A, et al. Central carbon metabolism of Saccharomyces cerevisiae in anaerobic, oxygen-limited and fully aerobic steady-state conditions and following a shift to anaerobic conditions. FEMS Yeast Res. 2008;8(1):140-54.
Wiebe, M. G., Rintala, E., Tamminen, A., Simolin, H., Salusjärvi, L., Toivari, M., Kokkonen, J. T., Kiuru, J., Ketola, R. A., Jouhten, P., Huuskonen, A., Maaheimo, H., Ruohonen, L., & Penttilä, M. (2008). Central carbon metabolism of Saccharomyces cerevisiae in anaerobic, oxygen-limited and fully aerobic steady-state conditions and following a shift to anaerobic conditions. FEMS Yeast Research, 8(1), 140-54.
Wiebe MG, et al. Central Carbon Metabolism of Saccharomyces Cerevisiae in Anaerobic, Oxygen-limited and Fully Aerobic Steady-state Conditions and Following a Shift to Anaerobic Conditions. FEMS Yeast Res. 2008;8(1):140-54. PubMed PMID: 17425669.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Central carbon metabolism of Saccharomyces cerevisiae in anaerobic, oxygen-limited and fully aerobic steady-state conditions and following a shift to anaerobic conditions. AU - Wiebe,Marilyn G, AU - Rintala,Eija, AU - Tamminen,Anu, AU - Simolin,Helena, AU - Salusjärvi,Laura, AU - Toivari,Mervi, AU - Kokkonen,Juha T, AU - Kiuru,Jari, AU - Ketola,Raimo A, AU - Jouhten,Paula, AU - Huuskonen,Anne, AU - Maaheimo,Hannu, AU - Ruohonen,Laura, AU - Penttilä,Merja, Y1 - 2007/04/10/ PY - 2007/4/12/pubmed PY - 2008/7/11/medline PY - 2007/4/12/entrez SP - 140 EP - 54 JF - FEMS yeast research JO - FEMS Yeast Res VL - 8 IS - 1 N2 - Saccharomyces cerevisiae CEN.PK113-1A was grown in glucose-limited chemostat culture with 0%, 0.5%, 1.0%, 2.8% or 20.9% O2 in the inlet gas (D=0.10 h(-1), pH 5, 30 degrees C) to determine the effects of oxygen on 17 metabolites and 69 genes related to central carbon metabolism. The concentrations of tricarboxylic acid cycle (TCA) metabolites and all glycolytic metabolites except 2-phosphoglycerate+3-phosphoglycerate and phosphoenolpyruvate were higher in anaerobic than in fully aerobic conditions. Provision of only 0.5-1% O2 reduced the concentrations of most metabolites, as compared with anaerobic conditions. Transcription of most genes analyzed was reduced in 0%, 0.5% or 1.0% O2 relative to cells grown in 2.8% or 20.9% O2. Ethanol production was observed with 2.8% or less O2. After steady-state analysis in defined oxygen concentrations, the conditions were switched from aerobic to anaerobic. Metabolite and transcript levels were monitored for up to 96 h after the transition, and this showed that more than 30 h was required for the cells to fully adapt to anaerobiosis. Levels of metabolites of upper glycolysis and the TCA cycle increased following the transition to anaerobic conditions, whereas those of metabolites of lower glycolysis generally decreased. Gene regulation was more complex, with some genes showing transient upregulation or downregulation during the adaptation to anaerobic conditions. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/17425669/Central_carbon_metabolism_of_Saccharomyces_cerevisiae_in_anaerobic_oxygen_limited_and_fully_aerobic_steady_state_conditions_and_following_a_shift_to_anaerobic_conditions_ L2 - https://academic.oup.com/femsyr/article-lookup/doi/10.1111/j.1567-1364.2007.00234.x DB - PRIME DP - Unbound Medicine ER -