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Detection of cytogenetic and DNA damage in peripheral erythrocytes of goldfish (Carassius auratus) exposed to a glyphosate formulation using the micronucleus test and the comet assay.
Mutagenesis. 2007 Jul; 22(4):263-8.M

Abstract

Glyphosate is a widely used broad-spectrum weed control agent. In the present study, an in vivo study on the genotoxic effects of a technical herbicide (Roundup) containing isopropylamine salt of glyphosate was carried out on freshwater goldfish Carassius auratus. The fish were exposed to three doses of glyphosate formulation (5, 10 and 15 ppm). Cyclophosphamide at a single dose of 5 mg/l was used as positive control. Analysis of micronuclei, nuclear abnormalities and DNA damage were performed on peripheral erythrocytes sampled at intervals of 48, 96 and 144 h posttreatment. Our results revealed significant dose-dependent increases in the frequencies of micronuclei, nuclear abnormalities as well as DNA strand breaks. Our findings also confirmed that the alkaline comet assay and nuclear deformations in addition to micronucleus test on fish erythrocytes in vivo are useful tools in determining the potential genotoxicity of commercial herbicides.

Authors+Show Affiliations

Department of Biology, Mersin University, Mersin 33342, Turkey. tcavas@mersin.edu.trNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17426049

Citation

Cavaş, Tolga, and Serpil Könen. "Detection of Cytogenetic and DNA Damage in Peripheral Erythrocytes of Goldfish (Carassius Auratus) Exposed to a Glyphosate Formulation Using the Micronucleus Test and the Comet Assay." Mutagenesis, vol. 22, no. 4, 2007, pp. 263-8.
Cavaş T, Könen S. Detection of cytogenetic and DNA damage in peripheral erythrocytes of goldfish (Carassius auratus) exposed to a glyphosate formulation using the micronucleus test and the comet assay. Mutagenesis. 2007;22(4):263-8.
Cavaş, T., & Könen, S. (2007). Detection of cytogenetic and DNA damage in peripheral erythrocytes of goldfish (Carassius auratus) exposed to a glyphosate formulation using the micronucleus test and the comet assay. Mutagenesis, 22(4), 263-8.
Cavaş T, Könen S. Detection of Cytogenetic and DNA Damage in Peripheral Erythrocytes of Goldfish (Carassius Auratus) Exposed to a Glyphosate Formulation Using the Micronucleus Test and the Comet Assay. Mutagenesis. 2007;22(4):263-8. PubMed PMID: 17426049.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of cytogenetic and DNA damage in peripheral erythrocytes of goldfish (Carassius auratus) exposed to a glyphosate formulation using the micronucleus test and the comet assay. AU - Cavaş,Tolga, AU - Könen,Serpil, Y1 - 2007/04/10/ PY - 2007/4/12/pubmed PY - 2007/9/18/medline PY - 2007/4/12/entrez SP - 263 EP - 8 JF - Mutagenesis JO - Mutagenesis VL - 22 IS - 4 N2 - Glyphosate is a widely used broad-spectrum weed control agent. In the present study, an in vivo study on the genotoxic effects of a technical herbicide (Roundup) containing isopropylamine salt of glyphosate was carried out on freshwater goldfish Carassius auratus. The fish were exposed to three doses of glyphosate formulation (5, 10 and 15 ppm). Cyclophosphamide at a single dose of 5 mg/l was used as positive control. Analysis of micronuclei, nuclear abnormalities and DNA damage were performed on peripheral erythrocytes sampled at intervals of 48, 96 and 144 h posttreatment. Our results revealed significant dose-dependent increases in the frequencies of micronuclei, nuclear abnormalities as well as DNA strand breaks. Our findings also confirmed that the alkaline comet assay and nuclear deformations in addition to micronucleus test on fish erythrocytes in vivo are useful tools in determining the potential genotoxicity of commercial herbicides. SN - 0267-8357 UR - https://www.unboundmedicine.com/medline/citation/17426049/Detection_of_cytogenetic_and_DNA_damage_in_peripheral_erythrocytes_of_goldfish__Carassius_auratus__exposed_to_a_glyphosate_formulation_using_the_micronucleus_test_and_the_comet_assay_ L2 - https://academic.oup.com/mutage/article-lookup/doi/10.1093/mutage/gem012 DB - PRIME DP - Unbound Medicine ER -