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Liquid chromatography-tandem mass spectrometric method for determination of the anti-inflammatory compound vicenin-2 in the leaves of L. ericoides Mart.
Biomed Chromatogr. 2007 Sep; 21(9):925-30.BC

Abstract

This paper reports a rapid and sensitive method for determination of the anti-inflammatory compound vicenin-2 in L. ericoides Mart. using liquid chromatography-tandem mass spectrometry. Separation of the compound of interest was performed on a VP-ODS(18) (150 x 2 mm, Shimadzu, Japan) column and a pre-column packed with GPV-ODS C(18) (5 x 2 mm, Sigma-Aldrich, USA) with acetonitrile-water (15:85) mobile phase containing 2% acetic acid using isocratic flow at 0.5 mL/min for 2 min. Multiple-reaction monitoring of vicenin-2 was performed using electrospray positive ionization. The linear calibration curves were generated using a concentration range of 5-2500 ng/mL with correlation coefficients >0.99. The values of limit of detection and limit of quantitation were found to be 1 and 5 ng/mL, respectively. The method developed based on LC-ESIMS/MS is advantageous because it permits the rapid and selective detection of vicenin-2. Furthermore, the method can be easily applied to the routine analysis of vicenin-2 in plant extracts using a minimal amount of sample.

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Authors+Show Affiliations

de Moraes SL
Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ave do Café s/n, CEP 14040-903, Monte Alegre, Ribeirão Preto, SP, Brasil.
Tomaz JC
No affiliation info available
Lopes NP
No affiliation info available

MeSH

Anti-Inflammatory AgentsApigeninAsteraceaeChromatography, LiquidGlucosidesPlant ExtractsPlant LeavesSensitivity and SpecificitySpectrometry, Mass, Electrospray IonizationTandem Mass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

17428019

Citation

de Moraes, Solange Leite, et al. "Liquid Chromatography-tandem Mass Spectrometric Method for Determination of the Anti-inflammatory Compound Vicenin-2 in the Leaves of L. Ericoides Mart." Biomedical Chromatography : BMC, vol. 21, no. 9, 2007, pp. 925-30.
de Moraes SL, Tomaz JC, Lopes NP. Liquid chromatography-tandem mass spectrometric method for determination of the anti-inflammatory compound vicenin-2 in the leaves of L. ericoides Mart. Biomed Chromatogr. 2007;21(9):925-30.
de Moraes, S. L., Tomaz, J. C., & Lopes, N. P. (2007). Liquid chromatography-tandem mass spectrometric method for determination of the anti-inflammatory compound vicenin-2 in the leaves of L. ericoides Mart. Biomedical Chromatography : BMC, 21(9), 925-30.
de Moraes SL, Tomaz JC, Lopes NP. Liquid Chromatography-tandem Mass Spectrometric Method for Determination of the Anti-inflammatory Compound Vicenin-2 in the Leaves of L. Ericoides Mart. Biomed Chromatogr. 2007;21(9):925-30. PubMed PMID: 17428019.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Liquid chromatography-tandem mass spectrometric method for determination of the anti-inflammatory compound vicenin-2 in the leaves of L. ericoides Mart. AU - de Moraes,Solange Leite, AU - Tomaz,José Carlos, AU - Lopes,Norberto Peporine, PY - 2007/4/13/pubmed PY - 2007/11/8/medline PY - 2007/4/13/entrez SP - 925 EP - 30 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 21 IS - 9 N2 - This paper reports a rapid and sensitive method for determination of the anti-inflammatory compound vicenin-2 in L. ericoides Mart. using liquid chromatography-tandem mass spectrometry. Separation of the compound of interest was performed on a VP-ODS(18) (150 x 2 mm, Shimadzu, Japan) column and a pre-column packed with GPV-ODS C(18) (5 x 2 mm, Sigma-Aldrich, USA) with acetonitrile-water (15:85) mobile phase containing 2% acetic acid using isocratic flow at 0.5 mL/min for 2 min. Multiple-reaction monitoring of vicenin-2 was performed using electrospray positive ionization. The linear calibration curves were generated using a concentration range of 5-2500 ng/mL with correlation coefficients >0.99. The values of limit of detection and limit of quantitation were found to be 1 and 5 ng/mL, respectively. The method developed based on LC-ESIMS/MS is advantageous because it permits the rapid and selective detection of vicenin-2. Furthermore, the method can be easily applied to the routine analysis of vicenin-2 in plant extracts using a minimal amount of sample. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/17428019/Liquid_chromatography_tandem_mass_spectrometric_method_for_determination_of_the_anti_inflammatory_compound_vicenin_2_in_the_leaves_of_L__ericoides_Mart_ L2 - https://doi.org/10.1002/bmc.828 DB - PRIME DP - Unbound Medicine ER -