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[Effect of Rapamycin on CD4(+) CD25(+) regulatory T cells in allo-transplantation tolerance model].

Abstract

AIM

To study the effect of Rapamycin(Rapa) on CD4(+) CD25(+) regulatory T cells in allo-transplantation tolerance model.

METHODS

The model of skin allo-transplantation was established. The recipient BALB/c mice were injected with allogeneic donor spleen cells from B6 on the day before grafting and with cyclosporine(CsA) after transplantation. T cells were purified on the day 14 from the tolerant group and cultured with Rapa and/or IL-2 in different concentrations in vitro. Mixed lymphocyte reaction (MLR) was used to analyze the specific recall reaction of T cells. Percentages of CD4(+) CD25(+) regulatory T cells were examined by flow cytometry (FCM). The expression of Foxp3 mRNA was measured by RT-PCR, and the level of IL-10 in supernatant of T cells cultured in vitro was determined by ELISA. BALB/c-SCID mice underwent allo-transplantation were given adoptive transfer of T cells treated with Rapa and/or IL-2, then the survival conditions of the grafted skin were observed daily.

RESULTS

CsA plus donor splenocyte injection can prolong allograft of BALB/c significantly (P<0.05). The number of CD4(+) CD25(+) regulatory T cells and the expression of Foxp3 mRNA for T cell in the tolerant group treated with Rapa and/or IL-2 were obviously increased. Adoptive transfusion of T cells from tolerant mice treated with IL-2/Rapa obviously prolonged the allograft survival time in allografted-SCID mice.

CONCLUSION

Rapa can increase the ratio of CD4(+) CD25(+) regulatory T cells in vitro and prolong the graft survival time obviously after adoptive immunity, and these effects are enhanced by low-dose of IL-2.

Authors+Show Affiliations

Institute of Experimental Nuclear Medicine, Medical School of Shandong University, Jinan 250012, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

17428387

Citation

Zheng, Yong-Xian, et al. "[Effect of Rapamycin On CD4(+) CD25(+) Regulatory T Cells in Allo-transplantation Tolerance Model]." Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, vol. 23, no. 4, 2007, pp. 327-30.
Zheng YX, Hou GH, Song J, et al. [Effect of Rapamycin on CD4(+) CD25(+) regulatory T cells in allo-transplantation tolerance model]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007;23(4):327-30.
Zheng, Y. X., Hou, G. H., Song, J., Zhang, C., & Liang, T. (2007). [Effect of Rapamycin on CD4(+) CD25(+) regulatory T cells in allo-transplantation tolerance model]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, 23(4), pp. 327-30.
Zheng YX, et al. [Effect of Rapamycin On CD4(+) CD25(+) Regulatory T Cells in Allo-transplantation Tolerance Model]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007;23(4):327-30. PubMed PMID: 17428387.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Rapamycin on CD4(+) CD25(+) regulatory T cells in allo-transplantation tolerance model]. AU - Zheng,Yong-Xian, AU - Hou,Gui-Hua, AU - Song,Jing, AU - Zhang,Chao, AU - Liang,Ting, PY - 2007/4/13/pubmed PY - 2009/5/1/medline PY - 2007/4/13/entrez SP - 327 EP - 30 JF - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology JO - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi VL - 23 IS - 4 N2 - AIM: To study the effect of Rapamycin(Rapa) on CD4(+) CD25(+) regulatory T cells in allo-transplantation tolerance model. METHODS: The model of skin allo-transplantation was established. The recipient BALB/c mice were injected with allogeneic donor spleen cells from B6 on the day before grafting and with cyclosporine(CsA) after transplantation. T cells were purified on the day 14 from the tolerant group and cultured with Rapa and/or IL-2 in different concentrations in vitro. Mixed lymphocyte reaction (MLR) was used to analyze the specific recall reaction of T cells. Percentages of CD4(+) CD25(+) regulatory T cells were examined by flow cytometry (FCM). The expression of Foxp3 mRNA was measured by RT-PCR, and the level of IL-10 in supernatant of T cells cultured in vitro was determined by ELISA. BALB/c-SCID mice underwent allo-transplantation were given adoptive transfer of T cells treated with Rapa and/or IL-2, then the survival conditions of the grafted skin were observed daily. RESULTS: CsA plus donor splenocyte injection can prolong allograft of BALB/c significantly (P<0.05). The number of CD4(+) CD25(+) regulatory T cells and the expression of Foxp3 mRNA for T cell in the tolerant group treated with Rapa and/or IL-2 were obviously increased. Adoptive transfusion of T cells from tolerant mice treated with IL-2/Rapa obviously prolonged the allograft survival time in allografted-SCID mice. CONCLUSION: Rapa can increase the ratio of CD4(+) CD25(+) regulatory T cells in vitro and prolong the graft survival time obviously after adoptive immunity, and these effects are enhanced by low-dose of IL-2. SN - 1007-8738 UR - https://www.unboundmedicine.com/medline/citation/17428387/[Effect_of_Rapamycin_on_CD4_+__CD25_+__regulatory_T_cells_in_allo_transplantation_tolerance_model]_ DB - PRIME DP - Unbound Medicine ER -