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Solubilization and characterization of a cell wall-bound trehalase from ascospores of the fission yeast Schizosaccharomyces pombe.
Microbiol Res. 2009; 164(3):304-11.MR

Abstract

The genome of the fission yeast Schizosaccharomyces pombe lacks sequence homologs to ath1 genes coding for acid trehalases in other yeasts or filamentous fungi. However, acid trehalase activity is present at the spore stage in the life cycle of the fission yeast. The enzyme responsible for this activity behaves as a surface enzyme covalently linked to the spore cell walls in both wild-type and ntp1 mutant strains devoid of neutral trehalase. Lytic treatment of particulated cell wall fractions allowed the solubilization of the enzyme into an active form. We have characterized this soluble enzyme and found that its kinetic parameters, optimum pH and temperature, thermal denaturation and salt responses are closely similar to other conventional acid trehalases. Hence, this rather unusual enzyme can be recognized as acid trehalase by its biochemical properties although it does not share genetic homology with other known acid trehalases. The potential role of such acid trehalase in the mobilization of trehalose is discussed.

Authors+Show Affiliations

Department of Genetics and Microbiology, Facultad de Biología, University of Murcia, Murcia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17428646

Citation

Vicente-Soler, J, et al. "Solubilization and Characterization of a Cell Wall-bound Trehalase From Ascospores of the Fission Yeast Schizosaccharomyces Pombe." Microbiological Research, vol. 164, no. 3, 2009, pp. 304-11.
Vicente-Soler J, Soto T, Madrid M, et al. Solubilization and characterization of a cell wall-bound trehalase from ascospores of the fission yeast Schizosaccharomyces pombe. Microbiol Res. 2009;164(3):304-11.
Vicente-Soler, J., Soto, T., Madrid, M., Núñez, A., Cansado, J., & Gacto, M. (2009). Solubilization and characterization of a cell wall-bound trehalase from ascospores of the fission yeast Schizosaccharomyces pombe. Microbiological Research, 164(3), 304-11.
Vicente-Soler J, et al. Solubilization and Characterization of a Cell Wall-bound Trehalase From Ascospores of the Fission Yeast Schizosaccharomyces Pombe. Microbiol Res. 2009;164(3):304-11. PubMed PMID: 17428646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Solubilization and characterization of a cell wall-bound trehalase from ascospores of the fission yeast Schizosaccharomyces pombe. AU - Vicente-Soler,J, AU - Soto,T, AU - Madrid,M, AU - Núñez,A, AU - Cansado,J, AU - Gacto,M, Y1 - 2007/04/10/ PY - 2006/06/13/received PY - 2006/10/03/revised PY - 2006/12/08/accepted PY - 2007/4/13/pubmed PY - 2009/6/9/medline PY - 2007/4/13/entrez SP - 304 EP - 11 JF - Microbiological research JO - Microbiol Res VL - 164 IS - 3 N2 - The genome of the fission yeast Schizosaccharomyces pombe lacks sequence homologs to ath1 genes coding for acid trehalases in other yeasts or filamentous fungi. However, acid trehalase activity is present at the spore stage in the life cycle of the fission yeast. The enzyme responsible for this activity behaves as a surface enzyme covalently linked to the spore cell walls in both wild-type and ntp1 mutant strains devoid of neutral trehalase. Lytic treatment of particulated cell wall fractions allowed the solubilization of the enzyme into an active form. We have characterized this soluble enzyme and found that its kinetic parameters, optimum pH and temperature, thermal denaturation and salt responses are closely similar to other conventional acid trehalases. Hence, this rather unusual enzyme can be recognized as acid trehalase by its biochemical properties although it does not share genetic homology with other known acid trehalases. The potential role of such acid trehalase in the mobilization of trehalose is discussed. SN - 1618-0623 UR - https://www.unboundmedicine.com/medline/citation/17428646/Solubilization_and_characterization_of_a_cell_wall_bound_trehalase_from_ascospores_of_the_fission_yeast_Schizosaccharomyces_pombe_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-5013(07)00041-9 DB - PRIME DP - Unbound Medicine ER -