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[Headspace solid-phase microextraction-gas chromatography-mass spectrometry for analysis of volatile components from Atractlodes macrocephala Koidz].
Se Pu. 2007 Jan; 25(1):43-7.SP

Abstract

Headspace solid-phase microextraction (HS-SPME) technique was employed to extract the volatile components from Chinese traditional medicine, Atractlodes macrocephala Koidz. The volatile components were isolated and identified successfully by gas chromatography-mass spectrometry (GC-MS). The results from HS-SPME-GC-MS were compared with those obtained from steam distillation-GC-MS (SD-GC-MS) with a good agreement. The volatiles were collected using a polydimethylsiloxane-divinylbenzene (PDMS-DVB) fiber by HS-SPME. The best response was obtained when the extraction temperature was 70 degrees C, the equilibrium time and extraction time were all 30 min and the desorption time was 4 min. Analysis was performed by GC-MS with a polysiloxane capillary column (30 m x 0.25 mm, film thickness 0.25 microm) using He as the carrier gas and a programmed temperature run. Forty-one components accounting for 90.81% were identified. The main components (relative amount more than 1%) of the samples by HS-SPME were atractylone (40.12%), gamma-elemene (14.73%), aromadendrene (13.05%), eudesma-4 (14), 11-diene (5.46%), caryophyllene (2.56%), patchoulene (2.55%), 6,10,11,11-tetramethyl-tricyclo [6.3.0.1(2,3)] undec-7-ene (2.11%), cedrene (1.48%), alpha-caryophyllene (1.48%) and selina-4 (14) -7 (11) diene-8-one (1.01%). By SD-GC-MS, 31 components accounting for 88.19% were identified and all these components could be extracted by SPME except trans-beta-ocimene which accounts only 0.10%. The results showed that the HS-SPME technique can be used to extract the the volatile components from Atractlodes macrocephala Koidz. in place of the traditional time-consuming SD.

Authors+Show Affiliations

Guo F
Research Center for Modernization of Chinese Herbal Medicine, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Huang L
No affiliation info available
Zhou S
No affiliation info available

MeSH

Drugs, Chinese HerbalGas Chromatography-Mass SpectrometrySolid Phase MicroextractionVolatilization

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

17432574

Citation

Guo, Fangqiu, et al. "[Headspace Solid-phase Microextraction-gas Chromatography-mass Spectrometry for Analysis of Volatile Components From Atractlodes Macrocephala Koidz]." Se Pu = Chinese Journal of Chromatography, vol. 25, no. 1, 2007, pp. 43-7.
Guo F, Huang L, Zhou S. [Headspace solid-phase microextraction-gas chromatography-mass spectrometry for analysis of volatile components from Atractlodes macrocephala Koidz]. Se Pu. 2007;25(1):43-7.
Guo, F., Huang, L., & Zhou, S. (2007). [Headspace solid-phase microextraction-gas chromatography-mass spectrometry for analysis of volatile components from Atractlodes macrocephala Koidz]. Se Pu = Chinese Journal of Chromatography, 25(1), 43-7.
Guo F, Huang L, Zhou S. [Headspace Solid-phase Microextraction-gas Chromatography-mass Spectrometry for Analysis of Volatile Components From Atractlodes Macrocephala Koidz]. Se Pu. 2007;25(1):43-7. PubMed PMID: 17432574.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Headspace solid-phase microextraction-gas chromatography-mass spectrometry for analysis of volatile components from Atractlodes macrocephala Koidz]. AU - Guo,Fangqiu, AU - Huang,Lanfang, AU - Zhou,Shaoyun, PY - 2007/4/17/pubmed PY - 2010/6/11/medline PY - 2007/4/17/entrez SP - 43 EP - 7 JF - Se pu = Chinese journal of chromatography JO - Se Pu VL - 25 IS - 1 N2 - Headspace solid-phase microextraction (HS-SPME) technique was employed to extract the volatile components from Chinese traditional medicine, Atractlodes macrocephala Koidz. The volatile components were isolated and identified successfully by gas chromatography-mass spectrometry (GC-MS). The results from HS-SPME-GC-MS were compared with those obtained from steam distillation-GC-MS (SD-GC-MS) with a good agreement. The volatiles were collected using a polydimethylsiloxane-divinylbenzene (PDMS-DVB) fiber by HS-SPME. The best response was obtained when the extraction temperature was 70 degrees C, the equilibrium time and extraction time were all 30 min and the desorption time was 4 min. Analysis was performed by GC-MS with a polysiloxane capillary column (30 m x 0.25 mm, film thickness 0.25 microm) using He as the carrier gas and a programmed temperature run. Forty-one components accounting for 90.81% were identified. The main components (relative amount more than 1%) of the samples by HS-SPME were atractylone (40.12%), gamma-elemene (14.73%), aromadendrene (13.05%), eudesma-4 (14), 11-diene (5.46%), caryophyllene (2.56%), patchoulene (2.55%), 6,10,11,11-tetramethyl-tricyclo [6.3.0.1(2,3)] undec-7-ene (2.11%), cedrene (1.48%), alpha-caryophyllene (1.48%) and selina-4 (14) -7 (11) diene-8-one (1.01%). By SD-GC-MS, 31 components accounting for 88.19% were identified and all these components could be extracted by SPME except trans-beta-ocimene which accounts only 0.10%. The results showed that the HS-SPME technique can be used to extract the the volatile components from Atractlodes macrocephala Koidz. in place of the traditional time-consuming SD. SN - 1000-8713 UR - https://www.unboundmedicine.com/medline/citation/17432574/[Headspace_solid_phase_microextraction_gas_chromatography_mass_spectrometry_for_analysis_of_volatile_components_from_Atractlodes_macrocephala_Koidz]_ DB - PRIME DP - Unbound Medicine ER -
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