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Study of the protective effect of ascorbic acid against the toxicity of stannous chloride on oxidative damage, antioxidant enzymes and biochemical parameters in rabbits.

Abstract

Stannous chloride (SnCl2) is a reducing chemical agent used in several man-made products. SnCl2 can generate reactive oxygen species (ROS). Therefore, the present study has been carried out to investigate the antioxidant action of l-ascorbic acid (AA) in minimizing SnCl2 toxicity on lipid peroxidation, antioxidant enzyme, and biochemical parameters in male New Zealand white rabbits. Animals were assigned to one of four treatment groups: 0mg AA and 0mg SnCl2/kg BW (control); 40 mg AA/kg BW; 20mg SnCl2/kg BW; 20mg SnCl2 plus 40 mg AA/kg BW. Rabbits were orally administered the respective doses every other day for 12 weeks. Results obtained showed that SnCl2 significantly (P<0.05) induced thiobarbituric acid-reactive substances (TBARS; the marker of lipid peroxidation) in plasma, while the activities of glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT), and the level of sulfhydryl groups (SH-group) were decreased (P<0.05) in blood plasma. Aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (AlP), acid phosphatase (AcP) and lactate dehydrogenase (LDH) activities were decreased (P<0.05). Stannous chloride significantly (P<0.05) increased the levels of plasma total lipid (TL), cholesterol, triglyceride (TG), low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL), glucose, urea and total bilirubin. On the other hand, the level of plasma high-density lipoprotein (HDL), total protein (TP), albumin (A) and globulin (G) were significantly (P<0.05) decreased. Ascorbic acid alone significantly decreased the levels of TBARS, lipids and urea, and increased the activities of GST, SOD and CAT, and the levels of SH-group and proteins. While the rest of the tested parameters were not affected. Also, the presence of AA with SnCl2 alleviated its harmful effects on most of the tested parameters. Therefore, the present results revealed that treatment with AA could minimize the toxic effects of stannous chloride.

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  • Authors+Show Affiliations

    ,

    Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt. yousefmokhtar@yahoo.com

    , ,

    Source

    Toxicology 235:3 2007 Jun 25 pg 194-202

    MeSH

    Animals
    Antioxidants
    Ascorbic Acid
    Catalase
    Creatinine
    Glutathione Transferase
    Lipid Peroxidation
    Lipids
    Male
    Oxidative Stress
    Rabbits
    Superoxide Dismutase
    Thiobarbituric Acid Reactive Substances
    Tin Compounds
    Urea
    Vitamins

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    17433520

    Citation

    Yousef, M I., et al. "Study of the Protective Effect of Ascorbic Acid Against the Toxicity of Stannous Chloride On Oxidative Damage, Antioxidant Enzymes and Biochemical Parameters in Rabbits." Toxicology, vol. 235, no. 3, 2007, pp. 194-202.
    Yousef MI, Awad TI, Elhag FA, et al. Study of the protective effect of ascorbic acid against the toxicity of stannous chloride on oxidative damage, antioxidant enzymes and biochemical parameters in rabbits. Toxicology. 2007;235(3):194-202.
    Yousef, M. I., Awad, T. I., Elhag, F. A., & Khaled, F. A. (2007). Study of the protective effect of ascorbic acid against the toxicity of stannous chloride on oxidative damage, antioxidant enzymes and biochemical parameters in rabbits. Toxicology, 235(3), pp. 194-202.
    Yousef MI, et al. Study of the Protective Effect of Ascorbic Acid Against the Toxicity of Stannous Chloride On Oxidative Damage, Antioxidant Enzymes and Biochemical Parameters in Rabbits. Toxicology. 2007 Jun 25;235(3):194-202. PubMed PMID: 17433520.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Study of the protective effect of ascorbic acid against the toxicity of stannous chloride on oxidative damage, antioxidant enzymes and biochemical parameters in rabbits. AU - Yousef,M I, AU - Awad,T I, AU - Elhag,F A, AU - Khaled,F A, Y1 - 2007/03/18/ PY - 2007/02/03/received PY - 2007/03/05/revised PY - 2007/03/15/accepted PY - 2007/4/17/pubmed PY - 2007/6/30/medline PY - 2007/4/17/entrez SP - 194 EP - 202 JF - Toxicology JO - Toxicology VL - 235 IS - 3 N2 - Stannous chloride (SnCl2) is a reducing chemical agent used in several man-made products. SnCl2 can generate reactive oxygen species (ROS). Therefore, the present study has been carried out to investigate the antioxidant action of l-ascorbic acid (AA) in minimizing SnCl2 toxicity on lipid peroxidation, antioxidant enzyme, and biochemical parameters in male New Zealand white rabbits. Animals were assigned to one of four treatment groups: 0mg AA and 0mg SnCl2/kg BW (control); 40 mg AA/kg BW; 20mg SnCl2/kg BW; 20mg SnCl2 plus 40 mg AA/kg BW. Rabbits were orally administered the respective doses every other day for 12 weeks. Results obtained showed that SnCl2 significantly (P<0.05) induced thiobarbituric acid-reactive substances (TBARS; the marker of lipid peroxidation) in plasma, while the activities of glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT), and the level of sulfhydryl groups (SH-group) were decreased (P<0.05) in blood plasma. Aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (AlP), acid phosphatase (AcP) and lactate dehydrogenase (LDH) activities were decreased (P<0.05). Stannous chloride significantly (P<0.05) increased the levels of plasma total lipid (TL), cholesterol, triglyceride (TG), low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL), glucose, urea and total bilirubin. On the other hand, the level of plasma high-density lipoprotein (HDL), total protein (TP), albumin (A) and globulin (G) were significantly (P<0.05) decreased. Ascorbic acid alone significantly decreased the levels of TBARS, lipids and urea, and increased the activities of GST, SOD and CAT, and the levels of SH-group and proteins. While the rest of the tested parameters were not affected. Also, the presence of AA with SnCl2 alleviated its harmful effects on most of the tested parameters. Therefore, the present results revealed that treatment with AA could minimize the toxic effects of stannous chloride. SN - 0300-483X UR - https://www.unboundmedicine.com/medline/citation/17433520/Study_of_the_protective_effect_of_ascorbic_acid_against_the_toxicity_of_stannous_chloride_on_oxidative_damage_antioxidant_enzymes_and_biochemical_parameters_in_rabbits_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0300-483X(07)00186-2 DB - PRIME DP - Unbound Medicine ER -