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Cd/Zn exposure interactions on metallothionein response in Eisenia fetida (Annelida, Oligochaeta).
Comp Biochem Physiol C Toxicol Pharmacol. 2007 May; 145(4):658-68.CB

Abstract

We studied metallothionein (MT) response in the manure worm Eisenia fetida after exposures to cadmium (Cd), zinc (Zn) or cadmium and zinc spiked media. MT was studied both at the protein level by Dot Immunobinding Assay, (DIA) and at the expression level by Northern blotting. Cd was highly accumulated by worms whereas Zn body concentration was regulated. In addition, Zn would limit Cd accumulation in worms exposed to low Cd concentrations (1 and 8 mg Cd kg(-1) of dry soil). Exposure to a mixture of Cd and Zn at high concentrations increased cytosolic MT levels. This increase would allow worms to regulate body Zn concentrations and also to limit Cd toxicity. Cd exposures increased gene expression of Cd-binding MT isoform (MT 2A) whereas Zn did not. However, when both metals were at high concentrations in the exposure medium, this expression was further increased. Several hypotheses are proposed to explain the results and the best approach to estimate metal exposure of this earthworm species is given. Further experiments have now to be performed to evaluate the usefulness of these MT responses for field contaminated soils toxicity assessment using this earthworm species.

Authors+Show Affiliations

Laboratoire d'Ecologie Numérique et d'Ecotoxicologie, UPRES EA 3570, FR 1818 CNRS, Université des Sciences et Technologies de Lille, F-59655 Villeneuve d'Ascq Cedex, France. sylvain.demuynck@univ-lillel.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17433784

Citation

Demuynck, S, et al. "Cd/Zn Exposure Interactions On Metallothionein Response in Eisenia Fetida (Annelida, Oligochaeta)." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 145, no. 4, 2007, pp. 658-68.
Demuynck S, Grumiaux F, Mottier V, et al. Cd/Zn exposure interactions on metallothionein response in Eisenia fetida (Annelida, Oligochaeta). Comp Biochem Physiol C Toxicol Pharmacol. 2007;145(4):658-68.
Demuynck, S., Grumiaux, F., Mottier, V., Schikorski, D., Lemière, S., & Leprêtre, A. (2007). Cd/Zn exposure interactions on metallothionein response in Eisenia fetida (Annelida, Oligochaeta). Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 145(4), 658-68.
Demuynck S, et al. Cd/Zn Exposure Interactions On Metallothionein Response in Eisenia Fetida (Annelida, Oligochaeta). Comp Biochem Physiol C Toxicol Pharmacol. 2007;145(4):658-68. PubMed PMID: 17433784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cd/Zn exposure interactions on metallothionein response in Eisenia fetida (Annelida, Oligochaeta). AU - Demuynck,S, AU - Grumiaux,F, AU - Mottier,V, AU - Schikorski,D, AU - Lemière,S, AU - Leprêtre,A, Y1 - 2007/03/12/ PY - 2006/11/20/received PY - 2007/02/27/revised PY - 2007/03/01/accepted PY - 2007/4/17/pubmed PY - 2007/7/21/medline PY - 2007/4/17/entrez SP - 658 EP - 68 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 145 IS - 4 N2 - We studied metallothionein (MT) response in the manure worm Eisenia fetida after exposures to cadmium (Cd), zinc (Zn) or cadmium and zinc spiked media. MT was studied both at the protein level by Dot Immunobinding Assay, (DIA) and at the expression level by Northern blotting. Cd was highly accumulated by worms whereas Zn body concentration was regulated. In addition, Zn would limit Cd accumulation in worms exposed to low Cd concentrations (1 and 8 mg Cd kg(-1) of dry soil). Exposure to a mixture of Cd and Zn at high concentrations increased cytosolic MT levels. This increase would allow worms to regulate body Zn concentrations and also to limit Cd toxicity. Cd exposures increased gene expression of Cd-binding MT isoform (MT 2A) whereas Zn did not. However, when both metals were at high concentrations in the exposure medium, this expression was further increased. Several hypotheses are proposed to explain the results and the best approach to estimate metal exposure of this earthworm species is given. Further experiments have now to be performed to evaluate the usefulness of these MT responses for field contaminated soils toxicity assessment using this earthworm species. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/17433784/Cd/Zn_exposure_interactions_on_metallothionein_response_in_Eisenia_fetida__Annelida_Oligochaeta__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(07)00090-7 DB - PRIME DP - Unbound Medicine ER -