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Oxidized low density lipoprotein receptor-1 mediates oxidized low density lipoprotein-induced apoptosis in human umbilical vein endothelial cells: role of reactive oxygen species.
Vascul Pharmacol. 2007 Jul; 47(1):1-9.VP

Abstract

Studies have shown that oxidized low density lipoprotein (ox-LDL) elicits both necrotic and apoptotic cell death and several mechanisms have been proposed. Ox-LDL induces reactive oxygen species (ROS), a second messenger that might be involved in apoptosis, formation in different types of cells including endothelial cells (ECs) and smooth muscle cells (SMCs). As lectin-like ox-LDL receptor-1 (LOX-1) was the main receptor for ox-LDL, this study was designed to determine whether the apoptosis induced by ox-LDL was mediated by LOX-1 in cultured human umbilical vein endothelial cells (HUVECs) and whether there is an association between LOX-1 mediated apoptosis and the production of ROS. After exposure to ox-LDL (50,100, and 150 microg/ml for 18 h), HUVECs exhibit typical apoptotic characteristics as determined by transmission electron microscopy and flow cytometry analysis in a dose-dependent pattern. Ox-LDL increases intracellular ROS formation including superoxide anion (O2-) and hydrogen peroxide (H2O2) in a dose-dependent and time-dependent manner. Pretreatment with anti-LOX-1 mAb, Vitamin C, apocynin or catalase significantly reduced ROS production and prevented ox-LDL-induced apoptosis, while indomethacin or allopurinol had no effect. These results suggest that LOX-1 mediates ox-LDL-induced apoptosis in endothelial cells and that ROS production and NADPH oxidase might play an important role in ox-LDL-induced apoptosis.

Authors+Show Affiliations

Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17433786

Citation

Chen, Xiu-ping, et al. "Oxidized Low Density Lipoprotein Receptor-1 Mediates Oxidized Low Density Lipoprotein-induced Apoptosis in Human Umbilical Vein Endothelial Cells: Role of Reactive Oxygen Species." Vascular Pharmacology, vol. 47, no. 1, 2007, pp. 1-9.
Chen XP, Xun KL, Wu Q, et al. Oxidized low density lipoprotein receptor-1 mediates oxidized low density lipoprotein-induced apoptosis in human umbilical vein endothelial cells: role of reactive oxygen species. Vascul Pharmacol. 2007;47(1):1-9.
Chen, X. P., Xun, K. L., Wu, Q., Zhang, T. T., Shi, J. S., & Du, G. H. (2007). Oxidized low density lipoprotein receptor-1 mediates oxidized low density lipoprotein-induced apoptosis in human umbilical vein endothelial cells: role of reactive oxygen species. Vascular Pharmacology, 47(1), 1-9.
Chen XP, et al. Oxidized Low Density Lipoprotein Receptor-1 Mediates Oxidized Low Density Lipoprotein-induced Apoptosis in Human Umbilical Vein Endothelial Cells: Role of Reactive Oxygen Species. Vascul Pharmacol. 2007;47(1):1-9. PubMed PMID: 17433786.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidized low density lipoprotein receptor-1 mediates oxidized low density lipoprotein-induced apoptosis in human umbilical vein endothelial cells: role of reactive oxygen species. AU - Chen,Xiu-ping, AU - Xun,Ke-li, AU - Wu,Qin, AU - Zhang,Tian-tai, AU - Shi,Jing-shan, AU - Du,Guan-hua, Y1 - 2007/01/30/ PY - 2006/09/01/received PY - 2007/01/18/accepted PY - 2007/4/17/pubmed PY - 2007/8/10/medline PY - 2007/4/17/entrez SP - 1 EP - 9 JF - Vascular pharmacology JO - Vascul Pharmacol VL - 47 IS - 1 N2 - Studies have shown that oxidized low density lipoprotein (ox-LDL) elicits both necrotic and apoptotic cell death and several mechanisms have been proposed. Ox-LDL induces reactive oxygen species (ROS), a second messenger that might be involved in apoptosis, formation in different types of cells including endothelial cells (ECs) and smooth muscle cells (SMCs). As lectin-like ox-LDL receptor-1 (LOX-1) was the main receptor for ox-LDL, this study was designed to determine whether the apoptosis induced by ox-LDL was mediated by LOX-1 in cultured human umbilical vein endothelial cells (HUVECs) and whether there is an association between LOX-1 mediated apoptosis and the production of ROS. After exposure to ox-LDL (50,100, and 150 microg/ml for 18 h), HUVECs exhibit typical apoptotic characteristics as determined by transmission electron microscopy and flow cytometry analysis in a dose-dependent pattern. Ox-LDL increases intracellular ROS formation including superoxide anion (O2-) and hydrogen peroxide (H2O2) in a dose-dependent and time-dependent manner. Pretreatment with anti-LOX-1 mAb, Vitamin C, apocynin or catalase significantly reduced ROS production and prevented ox-LDL-induced apoptosis, while indomethacin or allopurinol had no effect. These results suggest that LOX-1 mediates ox-LDL-induced apoptosis in endothelial cells and that ROS production and NADPH oxidase might play an important role in ox-LDL-induced apoptosis. SN - 1537-1891 UR - https://www.unboundmedicine.com/medline/citation/17433786/Oxidized_low_density_lipoprotein_receptor_1_mediates_oxidized_low_density_lipoprotein_induced_apoptosis_in_human_umbilical_vein_endothelial_cells:_role_of_reactive_oxygen_species_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1537-1891(07)00025-0 DB - PRIME DP - Unbound Medicine ER -