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Coenzyme Q10 prevents high glucose-induced oxidative stress in human umbilical vein endothelial cells.
Eur J Pharmacol. 2007 Jul 02; 566(1-3):1-10.EJ

Abstract

Hyperglycemia-induced oxidative stress plays a crucial role in the pathogenesis of vascular complications in diabetes. Although some clinical evidences suggest the use of an antioxidant reagent coenzyme Q10 in diabetes with hypertension, the direct effect of coenzyme Q10 on the endothelial functions has not been examined. In the present study, we therefore investigated the protective effect of coenzyme Q10 against high glucose-induced oxidative stress in human umbilical vein endothelial cells (HUVEC). HUVEC exposed to high glucose (30 mM) exhibited abnormal properties, including the morphological and biochemical features of apoptosis, overproduction of reactive oxygen species, activation of protein kinase Cbeta2, and increase in endothelial nitric oxide synthase expression. Treatment with coenzyme Q10 strongly inhibited these changes in HUVEC under high glucose condition. In addition, coenzyme Q10 inhibited high glucose-induced cleavage of poly(ADP-ribose) polymerase, an endogenous caspase-3 substrate. These results suggest that coenzyme Q10 prevents reactive oxygen species-induced apoptosis through inhibition of the mitochondria-dependent caspase-3 pathway. Moreover, consistent with previous reports, high glucose caused upregulation of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in HUVEC, and promoted the adhesion of U937 monocytic cells. Coenzyme Q10 displayed potent inhibitory effects against these endothelial abnormalities. Thus, we provide the first evidence that coenzyme Q10 has a beneficial effect in protecting against the endothelial dysfunction by high glucose-induced oxidative stress in vitro.

Authors+Show Affiliations

Department of Clinical Pharmacology, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan. htsuneki@pha.u-toyama.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17434478

Citation

Tsuneki, Hiroshi, et al. "Coenzyme Q10 Prevents High Glucose-induced Oxidative Stress in Human Umbilical Vein Endothelial Cells." European Journal of Pharmacology, vol. 566, no. 1-3, 2007, pp. 1-10.
Tsuneki H, Sekizaki N, Suzuki T, et al. Coenzyme Q10 prevents high glucose-induced oxidative stress in human umbilical vein endothelial cells. Eur J Pharmacol. 2007;566(1-3):1-10.
Tsuneki, H., Sekizaki, N., Suzuki, T., Kobayashi, S., Wada, T., Okamoto, T., Kimura, I., & Sasaoka, T. (2007). Coenzyme Q10 prevents high glucose-induced oxidative stress in human umbilical vein endothelial cells. European Journal of Pharmacology, 566(1-3), 1-10.
Tsuneki H, et al. Coenzyme Q10 Prevents High Glucose-induced Oxidative Stress in Human Umbilical Vein Endothelial Cells. Eur J Pharmacol. 2007 Jul 2;566(1-3):1-10. PubMed PMID: 17434478.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Coenzyme Q10 prevents high glucose-induced oxidative stress in human umbilical vein endothelial cells. AU - Tsuneki,Hiroshi, AU - Sekizaki,Naoto, AU - Suzuki,Takashi, AU - Kobayashi,Shinjiro, AU - Wada,Tsutomu, AU - Okamoto,Tadashi, AU - Kimura,Ikuko, AU - Sasaoka,Toshiyasu, Y1 - 2007/03/19/ PY - 2006/12/18/received PY - 2007/03/01/revised PY - 2007/03/06/accepted PY - 2007/4/17/pubmed PY - 2007/9/20/medline PY - 2007/4/17/entrez SP - 1 EP - 10 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 566 IS - 1-3 N2 - Hyperglycemia-induced oxidative stress plays a crucial role in the pathogenesis of vascular complications in diabetes. Although some clinical evidences suggest the use of an antioxidant reagent coenzyme Q10 in diabetes with hypertension, the direct effect of coenzyme Q10 on the endothelial functions has not been examined. In the present study, we therefore investigated the protective effect of coenzyme Q10 against high glucose-induced oxidative stress in human umbilical vein endothelial cells (HUVEC). HUVEC exposed to high glucose (30 mM) exhibited abnormal properties, including the morphological and biochemical features of apoptosis, overproduction of reactive oxygen species, activation of protein kinase Cbeta2, and increase in endothelial nitric oxide synthase expression. Treatment with coenzyme Q10 strongly inhibited these changes in HUVEC under high glucose condition. In addition, coenzyme Q10 inhibited high glucose-induced cleavage of poly(ADP-ribose) polymerase, an endogenous caspase-3 substrate. These results suggest that coenzyme Q10 prevents reactive oxygen species-induced apoptosis through inhibition of the mitochondria-dependent caspase-3 pathway. Moreover, consistent with previous reports, high glucose caused upregulation of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in HUVEC, and promoted the adhesion of U937 monocytic cells. Coenzyme Q10 displayed potent inhibitory effects against these endothelial abnormalities. Thus, we provide the first evidence that coenzyme Q10 has a beneficial effect in protecting against the endothelial dysfunction by high glucose-induced oxidative stress in vitro. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/17434478/Coenzyme_Q10_prevents_high_glucose_induced_oxidative_stress_in_human_umbilical_vein_endothelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(07)00335-4 DB - PRIME DP - Unbound Medicine ER -